Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals

Ishida, María Akíko; Rubinsky-Elefant, Guita; Ferreira, Antônio Walter; Hoshino‐Shimizu, Sumie; Vaz, Adelaide José

doi:10.1016/j.actatropica.2003.09.005

Cited by 94 publications

(71 citation statements)

References 28 publications

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“…Low-molecular-weight glycoproteins obtained from T. solium by affinity chromatography, considerably improved the specificity of ELISA screening tests, but a large amount of antigens is required (Tsang et al 1989). Recently, it has successfully been reported the use of low-molecular-weight peptides obtained from the vesicular fluid of T. crassiceps antigens (Tcra-VF) and T. crassiceps cysticercus glycoproteins (Tcra-GP and Tcra(18-14)-GP in ELISA and immunoblot assays , Ishida et al 2003. The Pontal of Paranapanema region is located in the west of the state of São Paulo and concentrates the highest number of "Landless Peasants' Settlements" of the country.…”

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confidence: 99%

“…Dias et al (1991) found in 311 samples of stools of humans living in the state of São Paulo an occurrence of 0.5% in Taenia sp. eggs; of which 273 were identified as T. saginata proglottidis.The ELISA screening test is commonly used for the serological diagnosis of cysticercosis, although it crossreacts with cestoda parasites such as Hymenolepis nana and Echinococcus granulosos (Garcia et al 2003, Ishida et al 2003. Low-molecular-weight glycoproteins obtained from T. solium by affinity chromatography, considerably improved the specificity of ELISA screening tests, but a large amount of antigens is required (Tsang et al 1989).…”

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confidence: 99%

“…Tcra(18-14)-GP in ELISA and immunoblot assays , Ishida et al 2003. The Pontal of Paranapanema region is located in the west of the state of São Paulo and concentrates the highest number of "Landless Peasants' Settlements" of the country.…”

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Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Prestes-Carneiro

Freitas

Zago

et al. 2006

Mem. Inst. Oswaldo Cruz

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In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants ' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52°36'12", latitude 22°17'12") The taeniosis-cysticercosis complex is an important cause of morbidity and mortality, widespread not only in most underdeveloped countries, but also in industrialized ones (Raether & Hanel 2003). There is strong evidence supporting the high prevalence of Taenia infection in humans from resource-poor areas, especially in rural areas with deficient sanitation, low hygienic standards, and unusual customs, such as consumption of raw pork (Phiri et al. 2003).Taenia solium and Taenia saginata are two taenids of great economic and medical importance, causing bovine and porcine cysticercosis and taeniosis in humans. The encysted larva, known as cysticercus from T. saginata and T. solium are found in cattle and swine, respectively, and the adult tapeworm of T. saginata and T. solium are found in man. The cycle is completed when eggs in proglottids shed from the human tapeworm are disseminated to the environment through feces, followed by the ingestion by cattle or swine, and then the viable cysts are eaten by man in undercooked beef (Lees et al. 2002). People are also infected by ingestion of T. solium or T. saginata eggs by contact with carriers or contaminated foods. Cysticercosis is an infection caused by cysticercus, the larval stage of Cestoda Family Taeniidae. Neurocysticercosis (NC), the nervous-system form of cysticercosis is caused by larvae of tapeworm T. solium (Carpio 2002).In Brazil, several previous studies have shown that NC in humans is an important public-health problem; although, very few reports on the prevalence and distribution of porcine cysticercosis have been published (Sakai et al. 2001). In the state of São Paulo, the frequency of anti-T. solium cysticercus antibodies in humans was determined in few counties (Vaz et al. 1990, Bragazza et al. 2002. Nonetheless, studies about the prevalence of T. saginata infection in humans or the presence of T. saginata cysticercus in cattle are scarce (Ungar & Germano 1992). Dias et al. (1991) found in 311 samples of stools of humans living in the state of São Paulo an occurrence of 0.5% in Taenia sp. eggs; of which 273 were identified as T. saginata proglottidis.The ELISA screening test is commonly used for the serological diagnosis of cysticercosis, although it crossreacts with cestoda parasites such as Hymenolepis nana and Echinococcus granulosos (Garcia et al. 2003, Ishida et al. 2003. Low-molecular-weight glycoproteins obtained from T. solium by affinity chromatography, considerably improved the specificity of ELISA screening tests, but a large amount of antigens is required (Tsang et al. 1989). Recently, it has successfully been reported the use of low-molecular-weight peptides obtained from the vesicular fluid of T. crassiceps antigens (Tcra-VF) and T. crassiceps cysticercus glycoproteins (Tcra-GP and Tcra(18-14)-GP in ELISA and immunoblot assays , Ishida e...

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Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Prestes-Carneiro

Freitas

Zago

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…The current result considered additional evidence of widely spread phenomenon of cross reaction among parasites (Abdel-Rahman and Abdel-Megeed 2000; Espindola Pardini et al 2002;Abdel-Rahman et al 2003;Ishida et al 2003;Ferrer et al 2003;Noeli et al 2005;Kandil et al 2005;Hassanain et al 2009;El-Moghazy and Abdel-Rahman 2012). Cross binding activities in experimentally infected rabbit sera with F. gigantica to TgLA are higher than to other two types; TgRH and TgMEA.…”

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confidence: 99%

Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis

Shaapan

Toaleb²,

Abdel-Rahman³

2013

J Parasit Dis

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In the current study, cross-reaction between two important zoonotic parasites; extracellular helminthes Fasciola gigantica and intracellular protozoa Toxoplasma gondii was proved by enzyme linked immunosorbent assay. Five antigens were used to identify and compare the cross-binding activities in the prepared antisera. Two F. gigantica antigens; adult flukes (FgA) and eggs (FgEA) were used to detect IgG in T. gondii naturally infected human sera (TgIHS) and experimentally infected sera of sheep (TgISS), mice (TgIMS) and rats (TgIRS). Three types of T. gondii antigens; RH (TgRHA), local sheep isolate (TgLA) and ME49 isolate (TgMEA) were used to detect cross binding activities in F. gigantica experimental infected rabbit sera (FgIRS) and F. gigantica naturally infected bovine sera (FgIBS). The cross-binding activities in the prepared antisera were strongly directed towards FgA and TgLA rather than the other antigens. The characterization of the five antigens using SDS-PAGE showed 4 common bands of FgA and TgLA; 165, 97, 76, and 65 kDa. While two common bands were observed between TgRHA, TgMEA and FgA; 165, and 65 kDa. Whereas, two common bands found between three types of T. gondii antigens and FgEA were identified; 165 and 65 kDa. The immunogenic cross-reactive bands between FgA and TgLA with F. gigantica infected bovine sera were identified by immunoblot. In FgA, the common immunogenic bands were 165, 65 and 14 kDa. While in TgLA, common immunogenic bands were 165 and 65 kDa. Whereas, the common immunogenic band between FgA and TgLA identified with T. gondii experimentally infected sheep sera was 65 kDa. The current research proves cross reaction between F. gigantica and T. gondii. One common band of 65 kDa showed broad immunogenic cross-reactivity with the developed antisera raising the prospect of being putative common immunodiagnostic candidate of both infections.

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“…We have previously studied sera from patients with different helminthiases against different helminth antigens used in ELISA and IB: cross-reactivities occurred mostly with the use of antigens belonging to phylogenetically related parasite species 13 .…”

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

Ishida

Peralta

Livramento

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy.

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Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals

Cited by 94 publications

References 28 publications

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

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