Helicobacter pylori treatment in the post-antibiotics era—searching for new drug targets

Roszczenko-Jasińska, Paula; Wojtyś, Marta Ilona; Jagusztyn-Krynicka, Elżbieta Katarzyna

doi:10.1007/s00253-020-10945-w

Cited by 72 publications

(96 citation statements)

References 148 publications

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“…It should be mentioned that gastric cancer is the third most deadly cancer in the world [ 1 ]. One of the risk factors confirmed for gastric cancer is infection with the bacterium H. pylori [ 2 , 3 , 4 , 5 , 6 ], which is a spiral-shaped Gram-negative bacterium that infects more than half of the world’s population [ 2 , 7 , 8 ]. This bacterium colonizes the gastric mucosa and the surfaces of the gastric epithelium.…”

Section: Introductionmentioning

confidence: 99%

Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients

Deptuła

Suprewicz

Daniluk

et al. 2021

IJMS

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Background: the molecular mechanism of gastric cancer development related to Helicobacter pylori (H. pylori) infection has not been fully understood, and further studies are still needed. Information regarding nanomechanical aspects of pathophysiological events that occur during H. pylori infection can be crucial in the development of new prevention, treatment, and diagnostic measures against clinical consequences associated with H. pylori infection, including gastric ulcer, duodenal ulcer, and gastric cancer. Methods: in this study, we assessed mechanical properties of children’s healthy and H. pylori positive stomach tissues and the mechanical response of human gastric cells exposed to heat-treated H. pylori cells using atomic force microscopy (AFM NanoWizard 4 BioScience JPK Instruments Bruker). Elastic modulus (i.e., the Young’s modulus) was derived from the Hertz–Sneddon model applied to force-indentation curves. Human tissue samples were evaluated using rapid urease tests to identify H. pylori positive samples, and the presence of H. pylori cells in those samples was confirmed using immunohistopathological staining. Results and conclusion: collected data suggest that nanomechanical properties of infected tissue might be considered as markers indicated H. pylori presence since infected tissues are softer than uninfected ones. At the cellular level, this mechanical response is at least partially mediated by cell cytoskeleton remodeling indicating that gastric cells are able to tune their mechanical properties when subjected to the presence of H. pylori products. Persistent fluctuations of tissue mechanical properties in response to H. pylori infection might, in the long-term, promote induction of cancer development.

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Section: Introductionmentioning

confidence: 99%

Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients

Deptuła

Suprewicz

Daniluk

et al. 2021

IJMS

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show abstract

“…Lender et al found a negative association between Hp infection and BMI level 31 . Hp infection could cause a series of gastric diseases, such as chronic gastritis 32 , and then lead to GC eventually 33 . When infected with Hp , subjects might suffer from chronic gastritis and other gastric diseases for a long time, even more than 10 years, which could cause their weight loss by affecting food intake or digestion.…”

Section: Discussionmentioning

confidence: 99%

Changes of Body Mass Index and Body Shape in relation to risk of Gastric Cancer: A population-based case-control study

et al. 2021

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Background: The results of previous studies are heterogeneous about the effect of body fatness on risk of gastric cancer (GC). Herein we investigated the effect of changes of BMI and body shape on risk of GC. Methods: A population-based case-control study enrolled 1989 controls and 937 GC cases. Logistic regression models were used to calculate odd ratios ( OR s) and 95% confidence intervals ( CI s) for BMI and body shape in association with GC risk, according to anatomical subsite, Laurén's classification, sex and Helicobacter pylori ( Hp ) infection. Results: Subjects with higher BMI or body shape 10 years before interview had a lower risk of GC regardless of anatomical subsite, Laurén's classification, and sex (all P for trend <0.05). But the relative risk patterns were different by Hp status. When checking the effect of changes of body fatness, in Hp + stratum, the OR s (95% CI ) were 0.40 (0.17-0.93) for subjects who were underweight at age 20 but had increased BMI afterwards, and 0.48 (0.32-0.73) for those of body shape 1/2 at age 20 but increased body shape subsequently, compared to subjects with stable BMI or body shape. When subjects had a normal BMI or 3/4 body shape at age 20, weight loss nearly doubled the risk of GC, and weight gain would decrease the risk. Conclusion: The association between body fatness and GC risk might differ by time point of measurement and Hp -infection status. Further, the influence of changes of body fatness might be different by baseline body fatness and Hp -infection status.

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“…The search for new drugs displaying activity against H. pylori is now a hotspot in the effective and safe control of this bacterium [ 11 ]. High hopes are associated with phytotherapy, which is the practice of using raw or processed plant products (flowers, leaves, stalks, roots, or seeds) for medicinal purposes [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

et al. 2021

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Helicobacter pylori is a Gram-negative bacterium that colonizes the stomach of about 60% of people worldwide. The search for new drugs with activity against H. pylori is now a hotspot in the effective and safe control of this bacterium. Therefore, the aim of this research was to determine the antibacterial activity of extracts from selected plants of the Papaveraceae family against planktonic and biofilm forms of the multidrug-resistant clinical strain of H. pylori using a broad spectrum of analytical in vitro methods. It was revealed that among the tested extracts, those obtained from Corydalis cheilanthifolia and Chelidonium majus were the most active, with minimal inhibitory concentrations (MICs) of 64 µg/mL and 128 µg/mL, respectively. High concentrations of both extracts showed cytotoxicity against cell lines of human hepatic origin. Therefore, we attempted to lower their MICs through the use of a synergistic combination with synthetic antimicrobials as well as by applying cellulose as a drug carrier. Using checkerboard assays, we determined that both extracts presented synergistic interactions with amoxicillin (AMX) and 3-bromopyruvate (3-BP) (FICI = 0.5) and additive relationships with sertraline (SER) (FICI = 0.75). The antibiofilm activity of extracts and their combinations with AMX, 3-BP, or SER, was analyzed by two methods, i.e., the microcapillary overgrowth under flow conditions (the Bioflux system) and assessment of the viability of lawn biofilms after exposure to drugs released from bacterial cellulose (BC) carriers. Using both methods, we observed a several-fold decrease in the level of H. pylori biofilm, indicating the ability of the tested compounds to eradicate the microbial biofilm. The obtained results indicate that application of plant-derived extracts from the Papaveraceae family combined with synthetic antimicrobials, absorbed into organic BC carrier, may be considered a promising way of fighting biofilm-forming H. pylori.

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Helicobacter pylori treatment in the post-antibiotics era—searching for new drug targets

Cited by 72 publications

References 148 publications

Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients

Nanomechanical Hallmarks of Helicobacter pylori Infection in Pediatric Patients

Changes of Body Mass Index and Body Shape in relation to risk of Gastric Cancer: A population-based case-control study

Antibiofilm and Antimicrobial-Enhancing Activity of Chelidonium majus and Corydalis cheilanthifolia Extracts against Multidrug-Resistant Helicobacter pylori

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