Helicases and Their Importance in Abiotic Stresses

Seraj, Zeba I.; Elias, Sabrina M.; Biswas, Sudip; Tuteja, Narendra

doi:10.1007/978-3-319-90318-7_6

Cited by 2 publications

(2 citation statements)

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“…The core domain consists of about 350-400 amino acids and 14 conserved motifs serving as their catalytic pockets (Umate et al, 2010;Passricha et al, 2018). The functional diversity of helicases may arise either due to differential binding patterns of the nucleic acids or from the variations in their N or C terminal domains (Jankowsky and Fairman, 2007;Seraj et al, 2018). Based on the structural and functional characteristics, helicases are divided into six super families (SF1 to SF6).…”

Section: Introductionmentioning

confidence: 99%

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Gain-of-function mutagenesis through activation tagging identifies XPB2 and SEN1 helicase genes as potential targets for drought stress tolerance in rice

et al. 2021

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We have earlier reported on the development of an activation tagged gain-of-function mutant population in an indica rice variety, BPT-5204 (Moin et al. 2016). Screening of these gain of function mutants for water-use efficiency (WUE) followed by physiological analyses revealed the activation of two helicases, ATP-dependent RNA (SEN1) and DNA (XPB2) encoding unwinding proteins in two different mutant lines. In the current study, we examined the roles of these genes in stable activation tagged mutants of rice for drought stress responses. Transcript profiling of SEN1 and XPB2 showed their significant up-regulation under various stresses (particularly ABA and PEG). The SEN1 and XPB2 tagged mutants exhibited reduced leaf wilting, improved revival efficiency, high chlorophyll and proline contents, profuse tillering, high quantum efficiency and yield-related traits in response to simulated drought (PEG) and hormone (ABA) treatments with respect to their controls. These observations were further validated under greenhouse conditions by periodic withdrawal of water. Germination of the seeds of these mutant lines indicates their ABA insensitivity under high ABA concentration. Also, the associated high up-regulation of stress-specific genes suggests that their drought tolerance might have been because of the coordinated expression of several stress responsive genes in these two mutants. Altogether, our results provided a firm basis for SEN1 and XPB2 as potential candidates for manipulation of drought tolerance and improving rice performance and yield under limited water conditions.

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Section: Introductionmentioning

confidence: 99%

“…Of them, a majority of the DNA and RNA helicases fall under SF1 and SF2 category. SF1 family of helicases are well characterized and SF2 is considered to be the largest family of helicases (Seraj et al, 2018;Passricha et al, 2018).…”

Section: Introductionmentioning

confidence: 99%