Heat Stress Impairs Maternal Endometrial Integrity and Results in Embryo Implantation Failure by Regulating Transport-Related Gene Expression in Tongcheng Pigs

Lian, Weisi; Gao, Dengying; Zhong, Qiqi; Hua, Renwu; Lei, Minggang

doi:10.3390/biom12030388

Cited by 7 publications

(4 citation statements)

References 53 publications

(45 reference statements)

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“…FUT8 disruption leads to growth retardation, early postnatal developmental death, and emphysema-like changes in the lungs [ 62 ]. MIPEP expression is up-regulated in response to heat stress [ 63 ], and FRMPD4 is highly expressed in pig breeds with high teat numbers [ 64 ]. In addition, SKIDA1 is related to the survival of human embryonic stem cells [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

Zong,

Wang,

Zhao

et al. 2023

J Animal Sci Biotechnol

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Background During approximately 10,000 years of domestication and selection, a large number of structural variations (SVs) have emerged in the genome of pig breeds, profoundly influencing their phenotypes and the ability to adapt to the local environment. SVs (≥ 50 bp) are widely distributed in the genome, mainly in the form of insertion (INS), mobile element insertion (MEI), deletion (DEL), duplication (DUP), inversion (INV), and translocation (TRA). While studies have investigated the SVs in pig genomes, genome-wide association studies (GWAS)-based on SVs have been rarely conducted. Results Here, we obtained a high-quality SV map containing 123,151 SVs from 15 Large White and 15 Min pigs through integrating the power of several SV tools, with 53.95% of the SVs being reported for the first time. These high-quality SVs were used to recover the population genetic structure, confirming the accuracy of genotyping. Potential functional SV loci were then identified based on positional effects and breed stratification. Finally, GWAS were performed for 36 traits by genotyping the screened potential causal loci in the F2 population according to their corresponding genomic positions. We identified a large number of loci involved in 8 carcass traits and 6 skeletal traits on chromosome 7, with FKBP5 containing the most significant SV locus for almost all traits. In addition, we found several significant loci in intramuscular fat, abdominal circumference, heart weight, and liver weight, etc. Conclusions We constructed a high-quality SV map using high-coverage sequencing data and then analyzed them by performing GWAS for 25 carcass traits, 7 skeletal traits, and 4 meat quality traits to determine that SVs may affect body size between European and Chinese pig breeds.

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Section: Resultsmentioning

confidence: 99%

Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

Zong,

Wang,

Zhao

et al. 2023

J Animal Sci Biotechnol

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“…Preimplantation embryos are particularly susceptible to, and can easily be disrupted by, HS [16]. This period is highly critical for the attachment of the developing embryo to the endometrium and, at this point, HS adversely affects the attachment of the embryo to the maternal tissues [88,89]. Before day 17 after insemination, the period during which interferon-tau (IFNT) is produced, embryos are more susceptible to HS, leading to embryo mortality and pregnancy loss because IFNT has the main role of sustaining a healthy pregnancy [90].…”

Section: The Impact Of Heat Stress On Developing Embryos and Newbornsmentioning

confidence: 99%

Heat Stress as a Barrier to Successful Reproduction and Potential Alleviation Strategies in Cattle

Khan

Mesalam

Heo

et al. 2023

Animals

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In recent decades, the adverse effects of global warming on all living beings have been unanimously recognized across the world. A high environmental temperature that increases the respiration and rectal temperature of cattle is called heat stress (HS), and it can affect both male and female reproductive functions. For successful reproduction and fertilization, mature and healthy oocytes are crucial; however, HS reduces the developmental competence of oocytes, which compromises reproduction. HS disturbs the hormonal balance that plays a crucial role in successful reproduction, particularly in reducing the luteinizing hormone and progesterone levels, which leads to severe problems such as poor follicle development with a poor-quality oocyte and problems related to maturity, silent estrus, abnormal or weak embryo development, and pregnancy loss, resulting in a declining reproduction rate and losses for the cattle industry. Lactating cattle are particularly susceptible to HS and, hence, their reproduction rate is substantially reduced. Additionally, bulls are also affected by HS; during summer, semen quality and sperm motility decline, leading to compromised reproduction. In summer, the conception rate is reduced by 20–30% worldwide. Although various techniques, such as the provision of water sprinklers, shade, and air conditioning, are used during summer, these methods are insufficient to recover the normal reproduction rate and, therefore, special attention is needed to improve reproductive efficiency and minimize the detrimental effect of HS on cattle during summer. The application of advanced reproductive technologies such as the production of embryos in vitro, cryopreservation during the hot season, embryo transfer, and timed artificial insemination may minimize the detrimental effects of HS on livestock reproduction and recover the losses in the cattle industry.

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“…Next, we investigated whether collagenase-1 treatment could also be effective in models known for exhibiting low embryo implantation rates, i.e., heat stress and embryo transfer (ET). Prolonged exposure to elevated environmental temperature reduces successful pregnancy rates [35][36][37] . In line, in our experimental model, keeping mice under heat stress conditions of 38ºC, we observed reduced embryo implantation rates with an average of only 5 implantation sites per female (Fig.…”

Section: Improved Implantation In Heat Stress and Embryo Transfermentioning

confidence: 99%

Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention

Zehorai

Gross

Shimshoni

et al. 2022

Preprint

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Successful embryo implantation is associated with intensive endometrium matrix remodeling, in which fibrillar collagens and matrix metalloproteinases play a key role. Here we report that topical treatment with a single dose of human collagenase-1 improves the rate of embryo implantation in murine models regardless of genetic background. Collagenase-1 treatment reduces endometrial collagen-fiber tension affecting their aligned configuration while preserving the overall integrity of the uterine tissue. The collagenase treatment causes enhanced local vascular permeability and creates an inflammatory-like environment, all of which are necessary for successful embryo implantation. We show that this specific treatment rescued heat stress and embryo transfer induced embryo failure in mice. Our findings highlight the clinical feasibility to improve endometrial receptivity for embryo implantation. This treatment approach may be used to improve livestock breeding and for assisted human reproduction medical treatments.

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Heat Stress Impairs Maternal Endometrial Integrity and Results in Embryo Implantation Failure by Regulating Transport-Related Gene Expression in Tongcheng Pigs

Cited by 7 publications

References 53 publications

Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

Heat Stress as a Barrier to Successful Reproduction and Potential Alleviation Strategies in Cattle

Enhancing Uterine Receptivity for Embryo Implantation through Controlled Collagenase Intervention

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