Heat shock proteins of &lt;em&gt;S. typhi&lt;/em&gt; and their reactivity with sera from patients with Typhoid fever

Tang, Seat-Wan; Panchanathan, Vijayaretnam; Naidu, B.R.; AbuBakar, Sazaly; Devi, Shamala; Puthucheary, S. D.; Pang, Tikki

doi:10.13181/mji.v7isupp1.1120

Cited by 9 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous work in our lab documented that heat shock proteins are induced and expressed in S. enterica serovar Typhi following thermal stress (31). This led us to investigate whether there were any polymorphisms or heterogeneity within the 1.6-kb groEL gene between serovar Typhi strains.…”

Section: Discussionmentioning

confidence: 96%

Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis

et al. 2002

View full text Add to dashboard Cite

PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.Salmonellae are the etiologic agents of different diseases collectively referred to as salmonellosis. Human salmonellosis can be divided into four syndromes: enteric fever (typhoid-like disease), gastroenteritis (food poisoning), bacteremia with or without gastroenteritis, and the asymptomatic carrier state.On a global scale, the incidence of typhoid fever is decreasing, while that of nontyphoidal salmonellosis is increasing, although both remain major health problems. The World Health Organization has estimated that annually there are close to 17 million cases of typhoid fever, with nearly 600,000 deaths, and 1.3 billion cases of acute gastroenteritis or diarrhea due to nontyphoidal salmonellosis, with 3 million deaths (8,23,26).To curb both typhoidal and nontyphoidal salmonellae, laboratory-based surveillance of human and animal infections is a necessary first step of any prevention strategy. Phenotypic methods play an important role in the identification to the genus level. Serotyping is used for primary typing of strains, while phage typing and antibiogram are used for subdivision of serotypes (33). However, serotyping of Salmonella is laborious due to the large number of recognized serotypes, i.e., over 2, 400 (1, 27).In addition, a number of molecular typing methods have also been used to try to improve the identification of salmonellae and also to differentiate strains below the level of serotypes. These DNA-related techniques include ribotyping (3), pulsedfield gel electrophoresis (PFGE) (18,20,32), IS200 fingerprinting (4), PCR-ribotyping (12), ribosomal DNA intergenic spacer amplification and heteroduplex analysis (9), amplified fragment length polymorphism (1, 21), automated 5Ј nuclease PCR assay (7), and random amplified polymorphic DNA analysis (30).In recent times, various molecular techniques that detect base sequence changes in bacteria have been used as tools in epidemiological typing. One of the most widely used techniques for the i...

show abstract

Section: Discussionmentioning

confidence: 96%

Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis

et al. 2002

View full text Add to dashboard Cite

show abstract

“…In the current study, we also identified PhoN, a PhoP-regulated phosphatase, as well as heat shock proteins GroEL and DnaK, as immunoreactive. Overall, heat shock proteins are often immunogenic (32,52), and immunization of mice with purified S. Typhi GroEL induces an IgG response and confers 70 to 90% protection against lethal intraperitoneal challenge with S. Typhi and S. Typhimurium in a murine model (31).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Anti- Salmonella enterica Serotype Typhi Antibody Responses in Bacteremic Bangladeshi Patients by an Immunoaffinity Proteomics-Based Technology

Charles

Sheikh

Krastins

et al. 2010

Clin Vaccine Immunol

View full text Add to dashboard Cite

Salmonella enterica serotype Typhi is the cause of typhoid fever and a human-restricted pathogen. Currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays to identify individuals with typhoid fever lack sensitivity and/or specificity. Identifying immunogenic S. Typhi antigens expressed during human infection could lead to improved diagnostic assays and vaccines. Here we describe a platform immunoaffinity proteomics-based technology (IPT) that involves the use of columns charged with IgG, IgM, or IgA antibody fractions recovered from humans bacteremic with S. Typhi to capture S. Typhi proteins that were subsequently identified by mass spectrometry. This screening tool identifies immunogenic proteins recognized by antibodies from infected hosts. Using this technology and the plasma of patients with S. Typhi bacteremia in Bangladesh, we identified 57 proteins of S. Typhi, including proteins known to be immunogenic (PagC, HlyE, OmpA, and GroEL) and a number of proteins present in the human-restricted serotypes S. Typhi and S. Paratyphi A but rarely found in broader-host-range Salmonella spp. (HlyE, CdtB, PltA, and STY1364). We categorized identified proteins into a number of major groupings, including those involved in energy metabolism, protein synthesis, iron homeostasis, and biosynthetic and metabolic functions and those predicted to localize to the outer membrane. We assessed systemic and mucosal anti-HlyE responses in S. Typhi-infected patients and detected anti-HlyE responses at the time of clinical presentation in patients but not in controls. These findings could assist in the development of improved diagnostic assays.Salmonella enterica serotype Typhi is a human-restricted pathogen that is the primary cause of enteric fever. It is estimated that S. Typhi infects over 20 million individuals and kills approximately 200,000 people globally each year (4). Currently, commercially available typhoid vaccines provide approximately 50 to 75% protection for 2 to 5 years (21), although an anti-typhoid Vi conjugate vaccine demonstrated 90% protection in 2-to 5-year-old children in a large field trial (23). Available and practical diagnostic tests for typhoid fever lack sensitivity and/or specificity (28). Identifying immunogenic S. Typhi antigens expressed during human infection could lead to improved diagnostic assays and vaccines.Infection with S. Typhi begins with the ingestion of contaminated water or food. The bacteria invade the gastrointestinal mucosa, translocate to the lymphoid follicles, where they survive and replicate within macrophages, and then disseminate via the bloodstream to the liver, spleen, and intestinal lymph nodes (14). The incubation period is typically 8 to 14 days (22), and symptoms include fever, abdominal pain, anorexia, weakness, potential complications of intestinal perforation, encephalopathy, and gastrointestinal bleeding (14,34). Clinical studies demonstrate that S. Typhi infection stimulates both an intestinal mucosa...

show abstract

“…In Salm. Typhi, heat shock treatment induced an inhibition of DnaK expression, while its basal level was very high (Tang et al 1997). A study is needed to clarify the inhibition of DnaK expression by gamma rays; however, according to Calini et al (2003) DnaK level could be a function of time and irradiation dose.…”

Section: Discussionmentioning

confidence: 99%

“…Although all the cells were killed at this radiation dose, we observed a substantial increase in the synthesis of the three Hsps proteins in E. coli and GroEL protein in the three other bacteria. This may be related to the fact that Hsps induction occurs very rapidly, and that it is detectable even after 5-10 s of exposure in a hostile environment (Tang et al 1997). Irradiator was used to deliver radiation at a dose rate of 4AE6 kGy h )1 and, therefore, the treatment lasted several minutes to reach the lethal dose.…”

Section: Discussionmentioning

confidence: 99%

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Caillet

Millette

Dussault

et al. 2008

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: The effects of gamma radiation on three heat shock proteins (Hsps) (GroEL, DnaK and GroES) synthesis in two Gram‐negative (Escherichia coli and Salmonella serotype Typhimurium) and two Gram‐positive (Staphylococcus aureus and Listeria monocytogenes) bacteria were investigated. Methods and Results: The bacterial strains were treated with three radiation doses to induce cell damage, to obtain a viable but nonculturable state, and to cause cell death. Western blot analysis and quantification of Hsps in bacteria were performed immediately after irradiation treatment. In the four foodborne pathogens, GroEL was strongly induced by gamma rays in a dose‐dependent manner, confirming the involvement of this protein in the cellular response to the stress generated by ionizing radiation. In addition, it was found that E. coli exposed to gamma radiation showed a significantly induction of DnaK and GroES proteins when compared with nonirradiated bacteria, whereas a GroES slight induction and a DnaK inhibition were observed in Salm. Typhimurium. Conclusions: The gamma rays influence the synthesis of Hsps in foodborne pathogen in a way that critically depends on the radiation dose. Significance and Impact of the Study: The study of stress response to several radiation doses was undertaken to elucidate how bacteria can survive in harsh conditions and cope with gamma radiation used to control foodborne pathogens and to characterize their adaptative response to this treatment.

show abstract

Heat shock proteins of <em>S. typhi</em> and their reactivity with sera from patients with Typhoid fever

Abstract: Heat shock proteins of S. typhi and their reactivity CLinicaL fertures and pathogenesis 225 CP.4

Cited by 9 publications

References 0 publications

Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis

Characterization of Salmonella Serovars by PCR-Single-Strand Conformation Polymorphism Analysis

Characterization of Anti- Salmonella enterica Serotype Typhi Antibody Responses in Bacteremic Bangladeshi Patients by an Immunoaffinity Proteomics-Based Technology

Effect of gamma radiation on heat shock protein expression of four foodborne pathogens

Contact Info

Product

Resources

About