Heat Shock Protein 70 and Anti-heat Shock Protein 70 Antibodies in Nasal Secretions of Patients with Chronic Rhinosinusitis

Abstract

Background:The issue of heat shock protein (HSP) 70 and anti-HSP70 antibodies in chronic rhinosinusitis (CRS) has never been explored.Objective:To determine the nasal secretion (NS) levels of HSP70 and anti-HSP70 antibodies in patients with CRS with nasal polyps (CRSwNP) and patients with CRS without nasal polyps (CRSsNP), and to evaluate their associations with CRS clinical severity and correlation with NS interleukin (IL), IL-5 and interferon λ.Methods:CRS severity was determined by Lund-Mackay scores. Levels of immunoglobulin E (IgE), IL-4, IL-5, interferon λ, HSP70, and anti-HSP70 antibody levels in NS were measured by enzyme-linked immunosorbent assay.Results:Forty-six patients with CRSsNP (25 women [54.3%] and 21 men [45.7%], mean [standard deviation {SD}]) age, 34.1 ± 12.3 years; 54 patients with CRSwNP (24 women [44.4%] and 30 men [55.6%], mean [SD] age, 37.9 ± 17.5 years). A group of 40 healthy subjects served as controls. Compared with the controls (with a mean [SD] NS HSP70 level of 0.05 ± 0.03 μg/mL), mean [SD] NS HSP70 levels in both the CRSsNP group (0.16 ± 0.07 μg/mL) and CRSwNP group (0.21 ± 0.10 μg/mL) were increased (p < 0.001). Similarly, the mean (SD) NS anti-HSP70 antibody levels were significantly higher in patients with CRSwNP (0.25 ± 0.09 optical density value [ODV]) compared with CRSsNP (0.13 ± 0.04 ODV) (p < 0.001) and healthy controls (0.14 ± 0.02 ODV) (p < 0.001). NS HSP70 in subjects with CRSwNP showed a significant positive correlation with the Lund-Mackay score (r = 0.31; p < 0.05). NS levels of either HSP70 or anti-HSP70 antibodies were strongly correlated with NS IL-4 in the CRSwNP group (r = 0.62, p < 0.001; and r = 0.69, p < 0.001, respectively).Conclusion:NS concentrations of HSP70 and secretory IgA anti HSP70 antibodies are increased in CRSwNP (but not in CRSsNP) and correlate positively with the Lund-Mackay score, NS IL-4, and NS IL-5.

Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity

“…[3][4][5] Recent studies demonstrated that apoptotic and necrotic cells release damage-associated molecules, such as heat shock proteins (HSPs), that promote early innate and adaptive immune responses. 6,7 HSP70 is the best-studied damage-associated molecule. It stimulates the maturation of dendritic antigen-presenting cells and activates the nuclear factor kappa B (NF-κB) pathway, which modulates production of several cytokines.…”

“…9 The concentrations of HSP70 in nasal secretions are increased in patients with NP and correlate positively with the severity of sinus disease and the levels of IL-4 and IL-5 in nasal fluid. 6,7 Many biomarkers of inflammation can be detected in nasal secretion samples and they can reflect the inflammatory status of the nasal mucosa and progression of mucosal disease. In the present study, we compared the levels of HSP70, eosinophil cationic protein (ECP), tryptase, substance P, and Clara cell protein…”

“…Recent studies demonstrated that apoptotic and necrotic cells release damage‐associated molecules, such as heat shock proteins (HSPs), that promote early innate and adaptive immune responses 6,7 . HSP70 is the best‐studied damage‐associated molecule.…”

“…HSP70 is the best‐studied damage‐associated molecule. It stimulates the maturation of dendritic antigen‐presenting cells and activates the nuclear factor kappa B (NF‐κB) pathway, which modulates production of several cytokines 6,7 . Several studies have indicated an important role of HSP70 in the pathogenesis of allergic diseases 8 .…”

Changed content of heat shock proteins and antibodies to them in blood and nasal mucosa cells in rhinites and rhinosinusites of different etiology

“…В последние годы отмечен значитель ный рост распространенности заболевания слизистой оболочки носа (риниты) и око лоносовых пазух (риносинуситы) [9]. Дан ные патологии чаще всего характеризуются синдромом, который включает заложен ность носа, ринорею, приступы чихания, головную боль и другие, что отрицательно сказывается на социальных, физических и психических аспектах жизни человека [1].…”