Heart estrogen receptor alpha: Distinct membrane and nuclear distribution patterns and regulation by estrogen

Ropero, Ana B.; Eghbali, Mansoureh; Minosyan, Tamara Y.; Tang, Guanghua; Toro, Ligia; Stefani, Enrico

doi:10.1016/j.yjmcc.2006.05.022

Cited by 58 publications

(50 citation statements)

References 45 publications

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“…The immunohistochemical changes in the receptors in the LCs could be a response to the low T biosynthesis rather than a causative factor for the hyperplasia. Prenatal DBP exposure might extend beyond the reproductive organs, to include the systemic physiology, because ERa is expressed in other tissues, for example, the efferent ducts and initial part of the epididymis, the cardiovascular system and bones (Gustafsson 1999;Mueller and Korach 2001;Simoncini et al 2002;Hess 2003;Arias-Loza et al 2006;Ropero et al 2006;Bolego et al 2010). Moreover, there is a need to find out the detailed cellular localization of ERa, ERb, and AR within the testis following prenatal exposure to DBP and their roles in testicular development and normal physiology.…”

Section: Discussionmentioning

confidence: 99%

Effects ofin UteroExposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats

et al. 2013

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Estrogens and androgens affect male and female reproductive systems. Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor a (ERa), estrogen receptor b (ERb), and androgen receptor (AR) in LCs of 5-, 7-, 9-, 14-, and 17-week-old Sprague-Dawley (srl) rats whose dams had been administered DBP intragastrically at 100 mg/kg/day or the vehicle (corn oil) from days 12 to 21 postconception. Immunohistochemical, Western blotting, and reverse transcription polymerase chain reaction analyses revealed that the expressions of ERa, ERb, and AR proteins and mRNAs in the DBP group were similar to those of the vehicle group at 5 and 7 weeks, but significantly higher ERa and lower ERb and AR levels were observed in the DBP group at 9 to 17 weeks. The rats prenatally exposed to DBP had seminiferous tubule degeneration and atypical hyperplasia of LCs during adulthood, which was associated with an increase in expression of ERa and a decrease of ERb and AR in the testis.

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Section: Discussionmentioning

confidence: 99%

Effects ofin UteroExposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats

et al. 2013

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“…Adult cardiomyocytes were isolated, fixed, and immunolabeled as previously described [14]. Cells were incubated with antibodies (Abs) against the following proteins: caveolin-1 (CAVg; BD Transduction Laboratories); caveolin-3 (CAV3; Santa Cruz); adenosine A 1 receptor (AD1R; Affinity Bioreagents); dihydropyridine receptors (DHPR; Alomone); and Na + -Ca 2+ exchanger (NCX1; R3F1 [8]).…”

Section: Cardiomyocyte Isolation and Fluorescence Microscopymentioning

confidence: 99%

“…Images were 3-D blind deconvolved using Auto Deblur (AutoQuant) as previously described [13,14]. To measure "colocalization" levels, 10-20 consecutive planes were analyzed in each cell.…”

Section: Digital Image Processing and Colocalization Analysismentioning

confidence: 99%

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Localization of sarcolemmal proteins to lipid rafts in the myocardium

et al. 2007

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The localization of sarcolemmal proteins within the membrane can have a dramatic effect on excitation-contraction coupling. We examine the localization of the Na + -Ca 2+ exchanger, the dihydropyridine receptor, and other proteins involved in excitation-contraction coupling in rat heart using biochemical and immunolocalization techniques. Specifically, we assess the distribution of proteins within the lipid raft fraction of the sarcolemma. We find that the distribution of proteins in lipid raft fractions is very dependent on the solubilization technique. A common technique using sodium carbonate/pH 11 to solubilize non-lipid raft proteins was inappropriate for use with sarcolemmal membranes. Use of Triton X-100 was more efficacious as a solubilization agent. A large majority of the Na + -Ca 2+ exchanger, Na + /K + -ATPase, and plasma membrane Ca 2+ pump are not present in lipid rafts. In contrast, most adenosine A 1 receptors and dihydropyridine receptors were in lipid raft fractions. Most of the adenosine A 1 receptors could be co-immunoprecipitated with caveolin indicating a localization to caveolae (a subclass of lipid rafts). In contrast, the dihydropyridine receptors could not be co-immunoprecipitated with caveolin. Most biochemical data were confirmed by high resolution immunolocalization studies. Using correlation analysis, only a small fraction of the Na + -Ca 2+ exchangers colocalized with caveolin whereas a substantial fraction of dihydropyridine and adenosine A 1 receptors did colocalize with caveolin. The most pertinent findings are that the Na + -Ca 2+ exchanger and the dihydropyridine receptor are in separate sarcolemmal subcompartments. These spatial relationships may be relevant for understanding excitation-contraction coupling.

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“…Estrogen's action is via its classic nuclear receptors and non-classic membrane receptors (63,(167)(168)(169)(170)(171)(172).Both ER and ER are expressed in the liver, muscle, and adipose tissue, as well as in several key regions of the hypothalamus of the central nervous system that have been linked to the control of peripheral glucose homeostasis. In particular, recent evidence points to neurons in the arcuate nucleus as critical regulators of glucose homeostasis (173).…”

Section: Estrogen Receptor Regulates Insulin Sensitivitymentioning

confidence: 99%