Healthy and diseased<i>in vitro</i>models of vascular systems

Hosseini, Vahid; Mallone, Anna; Nasrollahi, Fatemeh; Ostrovidov, Serge; Nasiri, Rohollah; Mahmoodi, Mahboobeh; Haghniaz, Reihaneh; Baidya, Avijit; Salek, M. Mehdi; Darabi, Mohammad Ali; Orive, Gorka; Shamloo, Amir; Dokmeci, Mehmet R.; Ahadian, Samad; Khademhosseini, Ali

doi:10.1039/d0lc00464b

Cited by 20 publications

(19 citation statements)

References 132 publications

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“…[33,34] The great attention on this research topic led to the development of quite many vascular models and fabrication methods in recent decades. [16] Still, no model could have been reproduced the biochemical reaction of blood vessels in vivo correctly because of the inflexibility of scaffolds and substrates. Permeability regulation of tight junction on ECs is triggered by the physical response of ECs occurred in the chemical reaction.…”

Section: Discussionmentioning

confidence: 99%

Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model

Itai

Onoe

2021

Adv Healthcare Materials

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Since the biochemical reaction of blood vessels plays an essential role in immune response or various diseases, in vitro vascular models have high demand from medical fields. However, vascular models often tend to be difficult to mimic the biomedical reaction faithfully because of the lack of implementation of the tissue deformation. Here, an inflammatory mediator-induced deformation reaction of a blood vessel on a flexibly deformable collagen hydrogel tube device is reproduced. A self-standing collagen tube enables the tissue to deform flexibly in biochemical reaction and achieves contraction both at tissue and cell level. The contraction of tissue relieves the stress between cells under reaction to maintain cellular junctions even tight junctions are broken. Also, the drug perfusion test with antihistamine chemical is easily performed due to the connector part and properly inhibits the inflammatory reaction. Moreover, the traction force on endothelial cells is analyzed as about 0.9 μN on two types of scaffolds with different stiffness. It is believed that the potential of the flexible tissue model to reproduce biochemical reactions can contribute to the fabrication of vascular tissue models mimicking in vivo in high similarity as a platform for biomedical researches and pharmacokinetic testing.

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Section: Discussionmentioning

confidence: 99%

Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model

Itai

Onoe

2021

Adv Healthcare Materials

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“…Despite clinical advances in the treatment of vascular disease, the mechanisms underlying the progression and regression of multifactorial arterial wall disease are still not fully understood [140,141]. The establishment of vascular disease models in vitro is conducive to understanding and studying its pathology, and provides a relatively convenient biological environment for the treatment of vascular diseases [2].…”

Section: Advanced Arterial Disease In Vitro Modelsmentioning

confidence: 99%

“…Second, it is important to fabricate an in vitro model of atherosclerosis tailored to the patient for diagnosis, which is conducive to achieving target-oriented treatment. In particular, atherosclerotic models should predict plaque size, location, and growth rate based on patient data to predict disease prognosis and provide a successful impact on specific treatments [2].…”

Section: Atherosclerosismentioning

confidence: 99%

“…When any member of the vascular system becomes disordered, a variety of fatal diseases and complications might be induced. For example, artery-relevant abnormalities (e.g., atherosclerosis, thrombosis, and aneurysm) can cause cardiovascular diseases, such as heart attack, stroke, and hemorrhage, which are the leading causes of death for millions of people worldwide each year [2,3].…”

Section: Introductionmentioning

confidence: 99%

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The Biofabrication of Diseased Artery In Vitro Models

et al. 2022

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As the leading causes of global death, cardiovascular diseases are generally initiated by artery-related disorders such as atherosclerosis, thrombosis, and aneurysm. Although clinical treatments have been developed to rescue patients suffering from artery-related disorders, the underlying pathologies of these arterial abnormalities are not fully understood. Biofabrication techniques pave the way to constructing diseased artery in vitro models using human vascular cells, biomaterials, and biomolecules, which are capable of recapitulating arterial pathophysiology with superior performance compared with conventional planar cell culture and experimental animal models. This review discusses the critical elements in the arterial microenvironment which are important considerations for recreating biomimetic human arteries with the desired disorders in vitro. Afterward, conventionally biofabricated platforms for the investigation of arterial diseases are summarized, along with their merits and shortcomings, followed by a comprehensive review of advanced biofabrication techniques and the progress of their applications in establishing diseased artery models.

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“…To better mimic the native environment, various strategies have been developed to expose ECs to shear in vitro, by using parallel plate flow chambers, plate-cone systems, microfluidic devices, or orbital shakers [16,17]. Many in vitro studies with ECs were performed using orbital shakers due to their availability in many labs, ease of use, and the large cell numbers that can be harvested for omics analyses, as summarized in Warboys et al [9].…”

Section: Introductionmentioning

confidence: 99%

Engineering solutions for biological studies of flow-exposed endothelial cells on orbital shakers

et al. 2022

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Shear stress is extremely important for endothelial cell (EC) function. The popularity of 6-well plates on orbital shakers to impose shear stress on ECs has increased among biologists due to their low cost and simplicity. One characteristic of such a platform is the heterogeneous flow profile within a well. While cells in the periphery are exposed to a laminar and high-velocity pulsatile flow that mimics physiological conditions, the flow in the center is disturbed and imposes low shear stress on the cells, which is characteristic of atheroprone regions. For studies where such heterogeneity is not desired, we present a simple cell-patterning technique to selectively prevent cell growth in the center of the well and facilitate the exclusive collection and analysis of cells in the periphery. This guarantees that cell phenotypes will not be influenced by secreted factors from cells exposed to other shear profiles nor that interesting results are obscured by mixing cells from different regions. We also present a multi-staining platform that compartmentalizes each well into 5 smaller independent regions: four at the periphery and one in the center. This is ideal for studies that aim to grow cells on the whole well surface, for comparison with previous work and minimal interference in the cell culture, but require screening of markers by immunostaining afterwards. It allows to compare different regions of the well, reduces antibody-related costs, and allows the exploration of multiple markers essential for high-content screening of cell response. By increasing the versatility of the 6-well plate on an orbital shaker system, we hope that these two solutions motivate biologists to pursue studies on EC mechanobiology and beyond.

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Healthy and diseasedin vitromodels of vascular systems

Abstract: A critical review of healthy and diseased in vitro models of the vascular system and in particular for atherosclerosis, aneurysm, and thrombosis.

Cited by 20 publications

References 132 publications

Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model

Flexibly Deformable Collagen Hydrogel Tube Reproducing Immunological Tissue Deformation of Blood Vessels as a Pharmacokinetic Testing Model

The Biofabrication of Diseased Artery In Vitro Models

Engineering solutions for biological studies of flow-exposed endothelial cells on orbital shakers

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