Head Size Determination and the Morphogenesis of Satellite Phage P4

Goldstein, Richard; Lengyel, Judith A.; Pruss, Gail J.; Barrett, Kathleen G.; Calendar, Richard; Six, Erich W.

doi:10.1007/978-3-642-66044-3_3

Cited by 17 publications

(22 citation statements)

References 33 publications

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“…Satellite P4 requires all of the 17 known helper P2 late gene products involved in P2 morphogenesis, yet P4 effects their assembly into an icosahedral capsid approximately one-third the overall volume of the capsid normally formed by bacteriophage P2 (6). In the absence of satellite P4, the P2 helper never assembles small satellite-size capsids from these same 17 late gene products.…”

Section: Discussionmentioning

confidence: 99%

“…In the absence of satellite P4, the P2 helper never assembles small satellite-size capsids from these same 17 late gene products. These differing assembly patterns suggest that the satellite P4 codes for a specific gene product(s) involved in directing the assembly of helper gene products to form the small satellite capsid (6,14). Determination of the identity and role of the satellite-specific sid + (size determination) gene product should provide a new approach to a central question in viral morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of small capsid size by P4 appears to be a tightly controlled non-self-assembly process, as is the case for other icosahedral viruses (7). Because indirect evidence suggests that the physical size of the P4 genome is not responsible for capsid size determination (6,8,9), it seems likely that satellite P4 codes for a specific gene product(s) that somehow interacts with the products provided by the helper to direct the formation of the smaller size icosahedral satellite capsid (see Fig. 1).…”

mentioning

confidence: 97%

“…The molecular weight of the P4 genome (7 X 106) is approximately one-third that of the P2 helper (5), and P4 can "direct" the assembly of gene products provided by the helper to form an icosahedral capsid one-third the volume of the helper capsid (6). Determination of small capsid size by P4 appears to be a tightly controlled non-self-assembly process, as is the case for other icosahedral viruses (7).…”

mentioning

confidence: 99%

“…This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. (6) have been corrected as a result of electron microscopic experiments described in this paper.…”

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confidence: 99%

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Determination of capsid size by satellite bacteriophage P4.

Shore¹,

Dehò²,

Tsipis³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Satellite bacteriophage P4 requires all morphogenic gene products provided by a helper phage, such as coliphage P2, to assemble its own capsid, which is one-third the volume of the larger helper capsid. We have isolated a satellite phage P4 sid (size determination) mutant that is unable to direct the assembly of the small wild-type-size P4 capsid. Instead, this mutant produces P4 plaque-forming units with large P2-size capsids which contain two or three copies of the P4 sid, genome. P4 sid, is evidently mutated in a protein that is specifically responsible for determining the precise size and symmetry of the structure into which the helper P2 gene products will assemble. In addition, we have found that the physical size of the genome does not appear to lay an essential role in the proper assembly of the icosahedral capsid, since the majority of the P4 sidl plaque-forming units do not contain a complete capsidful of DNA.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Determination of capsid size by satellite bacteriophage P4.

Shore¹,

Dehò²,

Tsipis³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Phage head size determination and head protein cleavage in vitro

et al. 1974

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Satellite phage P4 causes the head proteins of a helper phage, such as P2, t o form a small head. This small head is never found in cells infected by the helper virus alone. This finding, coupled with the dominance of P4 over its helper, indicates that the P4 genome has the potential for specific head size determination. Satellite phage P4 codes for a late protein which is found in the P4 head (45 copies/head). This protein may determine head size. Our finding that the small size of P4 DNA does not determine small head size in an in vitro DNA packaging system lends further support t o the idea that a P4 protein determines small head size.teins. Cleavage of the major head protein precursor can be observed in vitro after lysis of infected cells with lysozyme. The rate of this in vitro reaction is not affected by deoxyribonuclease; thus there cannot be a tight coupling between DNA packaging and the cleavage of the major capsid protein.Formation of P2 headlike structures is accompanied by cleavage of P2 head pro-

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Bacteriophage Structure

Eiserling¹

1979

Comprehensive Virology Volume 13: Structure and Assembly

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Head Size Determination and the Morphogenesis of Satellite Phage P4

Cited by 17 publications

References 33 publications

Determination of capsid size by satellite bacteriophage P4.

Determination of capsid size by satellite bacteriophage P4.

Phage head size determination and head protein cleavage in vitro

Bacteriophage Structure

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