HCK promotes glioblastoma progression by TGFβ signaling

Abstract

The hematopoietic cell kinase (HCK), a member of the Src family protein-tyrosine kinases (SFKs), is primarily expressed in cells of the myeloid and B lymphocyte lineages. Nevertheless, the roles of HCK in glioblastoma (GBM) remain to be examined. Thus, we aimed to investigate the effects of HCK on GBM development both in vitro and in vivo, as well as the underlying mechanism. The present study found that HCK was highly expressed in both tumor tissues from patients with GBM and cancer cell lines. HCK enhanced cell viability, proliferation, and migration, and induced cell apoptosis in vitro. Tumor xenografts results also demonstrated that HCK knockdown significantly inhibited tumor growth. Interestingly, gene set enrichment analysis (GSEA) showed HCK was closed associated with epithelial mesenchymal transition (EMT) and TGFβ signaling in GBM. In addition, we also found that HCK accentuates TGFβ-induced EMT, suggesting silencing HCK inhibited EMT through the inactivation of Smad signaling pathway. In conclusion, our findings indicated that HCK is involved in GBM progression via mediating EMT process, and may be served as a promising therapeutic target for GBM.

Deeper Insights on Alchornea cordifolia (Schumach. & Thonn.) Müll.Arg Extracts: Chemical Profiles, Biological Abilities, Network Analysis and Molecular Docking

“…It is of noteworthy interest that quercetin, apigenin, myricetin, and kaempferol were predicted to interact with pim-1 oncogene (PIM-1), estrogen receptor 1 (ESR1), and hemopoietic cell linase (HCK). These proteins have been proven to be useful for regulating tumor cell growth in preclinical models of cancer [ 65 , 66 , 67 , 68 ]. These putative interactions could mediate, at least partially, the observed anti-proliferative effects induced by A. cordifolia methanol extract.…”

Identifying RBM47, HCK, CD53, TYROBP, and HAVCR2 as Hub Genes in Advanced Atherosclerotic Plaques by Network-Based Analysis and Validation

“…But it remains to be clarified that RBM47 functions as an RNA editing factor for ApoB in immune cells and atherosclerotic plaques tissue. HCK is a member of the Src family of tyrosine kinases, which transmits membrane receptor signals and plays an important role in survival, proliferation migration and phagocytosis of immune cells (Wang et al, 2020 ). HCK has also been reported to participate in leukocyte adhesion and metastasis, which may promote atherosclerotic plaque formation (Medina et al, 2015 ).…”

­­Prognostic analysis of tumor mutation burden combined with immune infiltration of Thymic epithelial tumors

“…Moreover, HCK participated in the development of glioblastoma (GBM) by mediating the EMT process. This might become a promising therapeutic target for GBM [28].…”