HC-0B-06: Biodegradation of Hydrocarbons

Uche, Emenike Chijioke; Dadrasnia, Arezoo

doi:10.1007/978-981-10-0201-4_3

Cited by 5 publications

(3 citation statements)

References 72 publications

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“…Only a few bacteria can utilize hydrocarbons directly [ 65 , 66 , 67 , 68 ]. To overcome the problem of hydrophobicity in high-weight PH fractions, which limits the transfer to microbial cells, cultivation of surfactant-producing bacteria or addition of a biosurfactant to the contaminated substrate should be added [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

“…With a high concentration of PH, the drop in the biodegradation may result from nutrient limitation for microorganisms due to an increased number of heavy-weight hydrocarbons [ 69 ]. The petroleum hydrocarbons contain a lot of carbon, but they are very poor in other basic nutrients for microorganisms, especially nitrogen and phosphorus, which are needed for cellular metabolism [ 74 ], so they must be supplied with them.…”

Section: Discussionmentioning

confidence: 99%

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Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA0 (reference), PGA1 (1% PGA), PGA1B (1% PGA with Bacillus licheniformis), and PGA10 (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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“…Unconventional oil exploration has worsened the occurrence of this issue, as most crude oils produced by unconventional methods are heavy and extra-heavy and have elevated viscosities and densities. Heavy crude oils can have different amounts of light and heavy fractions, but they usually have less of the lighter aliphatic (saturated and unsaturated) hydrocarbons and more of the heavier aromatic, resin, and especially asphaltene compounds (Uche and Dadrasnia 2017 ). Because of its complex nature, asphaltene is the most polar and heaviest fraction of crude oil and could persist in the environment for decades, causing detrimental effects on contaminated soils, such as increased toxicity, decreased pore spaces, and alterations in electric conductivity, pH, texture, and bulk density (Brown et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Development of a highly tolerant bacterial consortium for asphaltene biodegradation in soils

Navas-Cáceres,

Parada,

Zafra

2023

Environ Sci Pollut Res

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Asphaltenes are the most polar and heavy fraction of petroleum, and their complex structure and toxicity make them resistant to biodegradation. The ability to tolerate high asphaltene concentrations is crucial to reducing the toxicity-related inhibition of microbial growth and improving their capacity for adaptation, survival, and biodegradation in soils highly contaminated with asphaltenes. This study developed a highly tolerant consortium for efficient asphaltene biodegradation in soils from 22 bacterial isolates obtained from heavy-crude oil-contaminated soils. Isolates corresponded to the Rhodococcus, Bacillus, Stutzerimonas, Cellulosimicrobium, Pseudomonas, and Paenibacillus genera, among others, and used pure asphaltenes and heavy crude oil as the only carbon sources. Surface plate assays were used to evaluate the tolerance of individual isolates to asphaltenes, and the results showed variations in the extension and inhibition rates with maximum tolerance levels at 60,000 mg asphaltenes l−1. Inhibition assays were used to select non-antagonistic bacterial isolates among those showing the highest tolerance levels to asphaltenes. A consortium made up of the five most tolerant and non-antagonistic bacterial isolates was able to degrade up to 83 wt.% out of 10,000 mg asphaltenes kg−1 in the soil after 52 days. Due to its biological compatibility, high asphaltene tolerance, and ability to utilise it as a source of energy, the degrading consortium developed in this work has shown a high potential for soil bioremediation and is a promising candidate for the treatment of aged soil areas contaminated with heavy and extra-heavy crude oil. This would be the first research to assess and consider extreme bacterial tolerance and microbial antagonism between individual degrading microbes, leading to the development of an improved consortium capable of efficiently degrading high amounts of asphaltenes in soil.

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