HBV Viral Load and Liver Enzyme Levels May Be Associated with the Wild<i> MBL2</i> AA Genotype

Moura, Tuane Carolina Ferreira; Amoras, Ednelza da Silva Graça; Araújo, Mauro Sérgio Moura de; Queiroz, Maria Alice Freitas; Conde, Simone Regina Souza da Silva; Demachki, Sâmia; Martins-Feitosa, Rosimar Neris; Machado, Luiz Fernando Almeida; Vallinoto, Izaura Maria Vieira Cayres; Ishak, Ricardo; Vallinoto, Antônio Carlos Rosário

doi:10.1155/2017/3718451

Cited by 5 publications

(5 citation statements)

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“…The inclusion and exclusion criteria in the present study were the same as those established by previous studies published by our research group ( 53 ), including an age older than 18 years, provision of consent by the participants through signing an informed consent form and the biological aspects inherent to the study.…”

Section: Methodsmentioning

confidence: 99%

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The −3279C>A and −924A>G polymorphisms in the FOXP3 Gene Are Associated With Viral Load and Liver Enzyme Levels in Patients With Chronic Viral Liver Diseases

et al. 2018

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The transcription factor FOXP3 is an essential marker of the development and activation of regulatory T cells (Tregs), which are cells specialized in the regulation and normal tolerance of the immune response. In the context of chronic viral liver diseases, Tregs participate in the maintenance of infections by promoting histopathological control and favor the immune escape of viral agents by suppressing the antiviral response. Single nucleotide polymorphisms (SNPs) may influence the function of FOXP3 in a number of pathological conditions. The present study sought to evaluate the influence of SNPs in the FOXP3 gene promoter region in patients with chronic viral liver diseases. Three SNPs (−3279C>A, −2383C>T, and −924A>G) were analyzed in groups of patients with chronic hepatitis C (CHC), active chronic hepatitis B (CHB-A), inactive chronic hepatitis B (CHB-I), and a healthy control group (CG) using real-time PCR. The frequencies of the polymorphic variants were compared between groups and correlated with liver histopathological characteristics and enzyme levels [i.e., alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transpeptidase (GGT)] obtained via biopsy and from the clinical records of the participating patients, respectively. For the −2338C>T SNP, no significant differences were found in the frequencies of variants between groups or in the histological findings. Significant associations between the polymorphisms and the CHB-I group were not established. The −3279C>A SNP was associated with altered viral loads (log10) and GGT levels in CHC patients with advanced stages of inflammatory activity and liver fibrosis. The −924A>G SNP was associated with altered viral loads (log10) and liver enzyme levels among CHB-A patients with milder inflammation and fibrosis. However, the frequencies of the −3279C>A and −924A>G polymorphisms were not directly associated with the histopathological profiles of the analyzed patients. These polymorphic variants may influence hepatic function in patients with chronic viral liver diseases but are not directly associated with the establishment of the degree of inflammatory activity and liver fibrosis.

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Section: Methodsmentioning

confidence: 99%

“…DNA was extracted from these samples based on a forensic method consisting of cell lysis, protein precipitation, DNA precipitation and DNA hydration steps, as described in a previous publication ( 53 ).…”

Section: Methodsmentioning

confidence: 99%

The −3279C>A and −924A>G polymorphisms in the FOXP3 Gene Are Associated With Viral Load and Liver Enzyme Levels in Patients With Chronic Viral Liver Diseases

et al. 2018

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“…From a metanalysis including 17 studies, of which two were Brazilian ( 46 , 47 ), the authors concluded that exon 1 variants leading to MBL deficiency (p.Gly54Asp, rs1800450; p.Gly57Glu, rs1800451, and p.Arg52Cys, rs5030737; known as B, C , and D variants and collectively named as O variants) have a dominant effect on the susceptibility to severe hepatitis B or liver cirrhosis, but not to chronic hepatitis B and hepatocellular carcinoma ( 48 ). In another Brazilian study, patients with active HBV infection and homozygous for the fully functional exon 1 MBL A variants presented a positive correlation between increased transaminase and HBV DNA levels and the presence of mild to moderate fibrosis ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Virus Infection Among Leprosy Patients: A Case for Polymorphisms Compromising Activation of the Lectin Pathway and Complement Receptors

et al. 2021

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Thousands of leprosy patients not only suffer from physical deformities, but also either have or have had hepatitis B virus (HBV) coinfection. Polymorphisms of the complement system modulate susceptibility to leprosy, but genetic susceptibility to past or present HBV infection is unknown. We used sequencing and multiplex sequence-specific PCR to genotype 72 polymorphisms of seven genes (MBL2, FCN1, FCN2, FCN3, MASP1, MASP2, C3) encoding components of the lectin pathway, and two genes encoding complement receptors (CR1, VSIG4) in 190 patients, of which 74 were positive for HBsAg and/or anti-HBc (HBV+, 93.2% with a resolved infection) and 116 lepromatous patients, and 408 HBV-blood donors. In addition, we tested for levels of proteins of the lectin pathway. We found no difference between serum concentrations of mannan-binding lectin (MBL), MBL-associated serine proteins (MASP-1, MASP-2, MASP-3, MAp44), ficolin-3 (FCN-3), soluble complement receptor 1 (sCR1) and MBL mediated C4 activation, measured by ELISA or TRIFMA in up to 167 HBV+ and HBV− patients. Haplotypes lowering protein levels or encoding dysfunctional proteins increased susceptibility to HBV infection: MBL2*LYQC (OR = 3.4, p = 0.02), MASP1*AC_CC (OR = 4.0, p = 0.015) and MASP2*1C2-l (OR = 5.4, p = 0.03). Conversely, FCN1*3C2 haplotype, associated with higher gene expression, was protective (OR = 0.56, P = 0.033). Other haplotypes associated with HBV susceptibility were: MASP2*2B1-i (OR = 19.25, P = 0.003), CR1*3A (OR = 2.65, P = 0.011) and VSIG4*TGGRCG (OR = 12.55, P = 0.014). Some polymorphisms in ficolin genes associated with lower protein levels increased susceptibility to leprosy/HBV infection: FCN*1 (OR = 1.66, P = 0.029), FCN2*GGGCAC (OR = 6.73, P = 0.008), and FCN3*del_del_C (OR = 12.54, P = 0.037), and to lepromatous disease/HBV infection: FCN2*TA (OR = 2.5, P = 0.009), whereas FCN2*MAG was associated with increased FCN-2 expression and resistance against coinfection (OR = 0.29, P = 0.026). These associations were independent of demographic factors and did not increase susceptibility to leprosy per se, except MASP2*1C2-l. Associations for FCN2, FCN3, MASP1, MASP2, and VSIG4 variants were also independent of each other. In conclusion, polymorphisms compromising activation of the lectin pathway of complement increase susceptibility to HBV infection, with ficolin polymorphisms playing a major role in modulating the susceptibility among leprosy patients.

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“…In the investigation of the main SNP in the MBL gene, patients with active HBV who had wildtype AA genotype had a positive correlation between increased levels of transaminases, HBV DNA and the presence of mild to moderate fibrosis [ 147 ]. Mutations in the FoxP3 gene, SNP-924 A > G, showed that G carrier status was associated with altered viral loads and liver enzyme levels in patients with chronic active hepatitis B (CHB-A) with inflammation and fibrosis.…”

Section: Hbv: the Impact Of The Geographical Area On The Prevalence Of The Virus In The Brazilian Amazonmentioning

confidence: 99%

The Epidemiological Impact of STIs among General and Vulnerable Populations of the Amazon Region of Brazil: 30 years of Surveillance

Machado

Fonseca

Queiroz

et al. 2021

Viruses

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Sexually transmitted infections (STIs) represent a worldwide public health problem and, although many of them are curable, they continue to be neglected, especially in areas with a low human development index, such as in the northern region of Brazil. This review describes the results of 30 years of studies at the Virus Laboratory at the Federal University of Pará, including the prevalence and molecular epidemiology of HIV-1, HTLV-1/2, HPV, HBV, Treponema pallidum and Chlamydia trachomatis among urban and non-urban populations, and also in vulnerable groups in the Brazilian Amazon. Control strategies and challenges in preventing STIs are discussed considering this immense geographic region, where essential health services are unable to reach the entire population, especially the most vulnerable, such as female sex workers, people who use illicit drugs, remnants of quilombolos and indigenous communities.

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HBV Viral Load and Liver Enzyme Levels May Be Associated with the Wild MBL2 AA Genotype

Cited by 5 publications

References 36 publications

The −3279C>A and −924A>G polymorphisms in the FOXP3 Gene Are Associated With Viral Load and Liver Enzyme Levels in Patients With Chronic Viral Liver Diseases

The −3279C>A and −924A>G polymorphisms in the FOXP3 Gene Are Associated With Viral Load and Liver Enzyme Levels in Patients With Chronic Viral Liver Diseases

Hepatitis B Virus Infection Among Leprosy Patients: A Case for Polymorphisms Compromising Activation of the Lectin Pathway and Complement Receptors

The Epidemiological Impact of STIs among General and Vulnerable Populations of the Amazon Region of Brazil: 30 years of Surveillance

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