Handy, rapid and multiplex detection of tumor markers based on encoded silica–hydrogel hybrid beads array chip

Yang, Zixue; Chen, Baoan; Wang, Heng; Xia, Guohua; Cheng, Jian; Pei, Xiao-Ping; Wang, Fei; Wen, Bin

doi:10.1016/j.bios.2013.04.014

Cited by 15 publications

(5 citation statements)

References 31 publications

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“…Composite microspheres can also be prepared by injecting hydrogel into PhC microspheres for multiplexed detection. 124 This composite microcarrier has stable encoding characteristics and avoids the nonspecific adsorption of biomolecules, thus achieving high sensitivity and specificity for the detection of tumor targets. [125][126] In recent years, Zhao et al combined molecular imprinting technology with a PhC encoding strategy to immobilize biomolecules on silica nanoparticles to prepare the microspheres using periodic ordered nanostructures of PhC as the structural templates (Figure 11F).…”

Section: Multiplexed Biosensingmentioning

confidence: 99%

Encoding microcarriers for biomedicine

et al. 2023

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High throughput biological analysis has become an important topic in modern biomedical research and clinical diagnosis. The flow encoding scheme based on the encoding microcarriers provides a feasible strategy for the multiplexed biological analysis. Different encoding characteristics invest the microcarriers with different encoding mechanisms. Biosensor analysis, drug screening, cell culture, and the construction and evaluation of bionic organ chips can be realized by decoding the microcarriers and quantifying the detection signal intensity. In this review, the encoding strategy of microcarriers was divided into the optical and non‐optical encoding approaches according to their encoding elements, and the research progress of the microcarrier encoding strategy was elaborated. Finally, we summarized the biomedical applications and predicted their future prospects.

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Section: Multiplexed Biosensingmentioning

confidence: 99%

Encoding microcarriers for biomedicine

et al. 2023

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“…Finally, Yang et al reported a strategy to detect tumor markers on encoded silica–hydrogel hybrid beads through a non-competitive immunoassay [124]. Labeled reported antibodies are incubated with the sample as well as particles functionalized with the protein of interest.…”

Section: Multiplex Biosensing On Hydrogel Particle Arraysmentioning

confidence: 99%

Hydrogel microparticles for biosensing

Goff

Srinivas

Hill³

et al. 2015

European Polymer Journal

175

144

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Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field.

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“…In this case, the duplex assay showed high selectivity in detecting the chosen targets with a good linear correlation at concentrations up to 500 ng/mL. In the same way, Yang et al realized a non-competitive immunoassay on encoded silica–hydrogel hybrid beads for the detection of tumor markers [80]. The fluorescence of encoded beads with labeled antibodies were measured before and after, compared to the levels in solution, and reported to a calibration curve for each marker.…”

Section: Microgels and Hydrogel Microparticles In Multiplex Assaysmentioning

confidence: 99%

Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets

Battista

Causa

Netti

2017

Gels

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Hydrogels, and in particular microgels, are playing an increasingly important role in a diverse range of applications due to their hydrophilic, biocompatible, and highly flexible chemical characteristics. On this basis, solution-like environment, non-fouling nature, easy probe accessibility and target diffusion, effective inclusion of reporting moieties can be achieved, making them ideal substrates for bio-sensing applications. In fact, hydrogels are already successfully used in immunoassays as well as sensitive nucleic acid assays, also enabling hydrogel-based suspension arrays. In this review, we discuss key parameters of hydrogels in the form of micron-sized particles to be used in sensing applications, paying attention to the protein and oligonucleotides (i.e., miRNAs) targets as most representative kind of biomarkers.

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Handy, rapid and multiplex detection of tumor markers based on encoded silica–hydrogel hybrid beads array chip

Cited by 15 publications

References 31 publications

Encoding microcarriers for biomedicine

Encoding microcarriers for biomedicine

Hydrogel microparticles for biosensing

Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets

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