Hamster-Tropic Sarcomagenic and Nonsarcomagenic Viruses Derived from Hamster Tumors Induced by the Gross Pseudotype of Moloney Sarcoma Virus

Kelloff, Gary J.; Huebner, Robert J.; Lee, Yong Ki; Toni, Robert; Gilden, Raymond V.

doi:10.1073/pnas.65.2.310

Cited by 42 publications

(22 citation statements)

References 16 publications

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“…In contrast, infectious viruses capable of serial propagation have been isolated from hamsters only on rare occasions, mainly from tumors induced by murine sarcoma viruses (2)(3)(4)(5). These viruses, termed "hamster specific", have an altered host range and do not share envelope or groupspecific (gs) antigens with the murine C-type viruses (6,7).…”

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confidence: 99%

Activation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens

Freeman¹,

Kelloff

Gilden

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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Cell cultures of Syrian hamster -embryo were treated for 7 days with selected chemicals. Certain cultures were morphologically transformed by three different chemical preparations and yielded cell lines that subsequently produced malignant tumors in hamsters.Although the cell lines were negative for infectious virus before inoculation into animals, hamster-specific C-type RNA virus was isolated from tumors or from cell lines derived from the tumors. Since infectious C-type viruses are usually not demonstrable in hamster tissues of normal or tumor origin, we conclude that the chemical treatment and activation of the viruses are related events.Infectious leukemia viruses (C-type, RNA) have been isolated many times from different strains of chickens, mice, and cats (1). In contrast, infectious viruses capable of serial propagation have been isolated from hamsters only on rare occasions, mainly from tumors induced by murine sarcoma viruses (2-5). These viruses, termed "hamster specific", have an altered host range and do not share envelope or groupspecific (gs) antigens with the murine C-type viruses (6, 7). Nonsarcomagenic C-type viruses, isolated from stocks of the hamster sarcoma virus by endpoint dilution (5) or cloning techniques (8), share a gs antigen (7) with a noninfectious hamster C-type virus that was detected in a spontaneous lymphoma (9). The above data were interpreted as indicating in vivo rescue of the murine sarcoma virus genome by a hamster-specific leukemia virus (HaLV). Leukemia or integrated state in hamsters, and if so, how prevalent is the viral genome? In this paper we present evidence that the latent or repressed HaLV genome may be widely disseminated in hamster colonies, but can be activated and isolated only under certain conditions. PROCEDURES AND RESULTS Cell transformation and tumor inductionCell cultures prepared from an early passage of hamster (LSH and NIH strains)-embryo cells were treated for 7 days with 0.1 ug/ml of the chemical carcinogen 3-methylcholanthrene, or with 0.1 or 1.0 yg/ml of certain fractions of cigarette-smoke condensate (12) dissolved in acetone and diluted in Eagle's Minimal Essential Medium with 10% fetalcalf serum. After the initial 7-day treatment, the chemicals were removed permanently and the cultures were subdivided as needed. Complete details of culture derivation and chemical treatment have been published (13). As summarized in Table 1, six of the chemically treated cell lines became transformed, based on changes in growth rates and morphological alterations after 5-10 subcultures (Figs. 1 and 2). The control cell lines remained normal; however, there was a marked variation in the growth characteristics of the cell pools used, with the F695 control culture becoming terminal after a few passages, and the F559 and F839 control cultures continuing to grow for at least 30 passages. Due to their lack of growth in culture, large numbers of F695 control cells were not available for inoculation into newborn hamsters. On the other hand, F839 and F559 con...

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confidence: 99%

Activation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens

Freeman¹,

Kelloff

Gilden

et al. 1971

Proc. Natl. Acad. Sci. U.S.A.

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“…This has been suggested from immunological studies (Freeman et al, 1974), and is also apparent from the relatively low number of C-type particles thus far isolated from hamsters, as well as from the biochemical and biological deficiencies frequently associated with such hamster par-ticles (Peebles, Haapala and Gazdar, 1972;Somers et al, 1973;Gazdar et al, 1973;Verma et al, 1974). Moreover, the oncogenesis of truly indigenous hamster C-type viruses is rather doubtful, because leukaemogenesis has been demonstrated only in one case (Graffi et at., 1968) and because the so-called hamster sarcomagexic agents are in fact pseudotypes derived from tumours induced in hamsters by murine sarcoma viruses (Bassin et at., 1968;Kelloff et at., 1970;Sarma, Log and Gilden, 1970). In view of the foregoing, it is evident that additional study is required to elucidate the nature of the endogenous C-type viruses of the hamster.…”

Section: The Role Of Inert Foreign Bodies In the Pathogenesis Of Cancermentioning

confidence: 99%

Iododeoxyuridine activation of oncornavirus-like particles of hamster origin

Yaniv¹,

Eylan²

1977

Br J Cancer

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“…Several workers have isolated hamster-specific sarcoma viruses from hamster tumours induced by murine sarcoma viruses (Bassin et al I968;Kelloff et al I97oa;Klement et al ~969;Sarma, Log & Gilden, ~97o;Perk et al 1969). Such viruses are no longer active in mice or mouse tissue cultures.…”

Section: Introductionmentioning

confidence: 99%

Envelope Antigen Relationships among Three Hamster-specific Sarcoma Viruses and a Hamster-specific Helper Virus

Kelloff

Huebner

Chang

et al. 1970

Journal of General Virology

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SUMMARYThe immunological relationships among three hamster-specific sarcoma viruses and a non-sarcomagenic virus were determined by neutralization and interference tests. These viruses were all derived from hamster tumours induced originally by murine sarcoma viruses but were no longer capable of growth on mouse cells. The four viruses shared at least one envelope antigen responsible for neutralization reactions. Specific interference for the sarcoma viruses was obtained in cultures infected with the non-sarcomagenic virus. In both assays these viruses were clearly distinguishable from murine C-type viruses.

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Hamster-Tropic Sarcomagenic and Nonsarcomagenic Viruses Derived from Hamster Tumors Induced by the Gross Pseudotype of Moloney Sarcoma Virus

Cited by 42 publications

References 16 publications

Activation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens

Activation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens

Iododeoxyuridine activation of oncornavirus-like particles of hamster origin

Envelope Antigen Relationships among Three Hamster-specific Sarcoma Viruses and a Hamster-specific Helper Virus

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