Halogenated pesticide transformation by a laccase–mediator system

Torres‐Duarte, Cristina; Román, Rosa; Tinoco, Raunel; Vázquez-Duhalt, Rafael

doi:10.1016/j.chemosphere.2009.07.039

Cited by 112 publications

(52 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar enzymes concentration has been successfully applied also by other authors (Cabana et al, 2007b;Torres-Duarte et al, 2009), but in few cases, very high enzymatic loads were necessary. Noteworthy, 5000-800 U/L of laccases were required to treat naproxen, diclofenac, hormones, bisphenol A and triclosan (Lloret et al, 2010;Murugesan et al, 2010;Nakamura and Mtui, 2003), towards which laccases of T. pubescens MUT 2400 reduced up to 60% of the initial concentration already at 100 U/L.…”

Section: Minimal Effective Concentration (Mec)mentioning

confidence: 85%

“…In the absence of specific natural or synthetic mediators, purified fungal laccases are ineffective on triclosan (Cabana et al, 2007b;Murugesan et al, 2010), oxybenzone and several organohalogenated pesticides (Torres-Duarte et al, 2009). Since synthetic mediators were not added in this study, mediators produced by T. pubescens MUT 2400 still present in the crude extract may be responsible of the high removal yields (above 50%) obtained for these compounds.…”

Section: Minimal Effective Concentration (Mec)mentioning

confidence: 98%

See 1 more Smart Citation

Removal of micropollutants by fungal laccases in model solution and municipal wastewater: evaluation of estrogenic activity and ecotoxicity

Spina

Cordero

Schilirò

et al. 2015

Journal of Cleaner Production

View full text Add to dashboard Cite

a b s t r a c tThis study describes a multidisciplinary approach that investigates the breakdown potential of a laccase mediated system from Trametes pubescens MUT 2400 against several micropollutants including already recognized endocrine disrupting chemicals at their natural residual concentrations (from mg/L up to ng/ L).In model solution, the chemical speciation focused on a mixture of 18 analytes and adopted stir bar sorptive extraction with directed in-situ derivatization followed by gas chromatography and mass spectroscopy analysis. The method's key performance parameters were evaluated in consideration of the chemical peculiarities and complexity of real wastewaters: precision, accuracy, estimated working range extended to a wide residual concentration interval (10 ng/L to100 mg/L) indicated its fitness for purpose. Laccases were extremely active towards all the target compounds, both in term of removal yields and rate. The maximal percentage of removal was obtained for 4-t-butylphenol, 2-hydroxybiphenyl, 4-noctylphenol, salicylic acid and estrone (percentage of removal above 90%). Enzymes concentration played a central role and in most of the cases, the catalyzed reactions were very fast: the initial concentration of 9 compounds was halved within the first 3 h.The laccase-mediated treatment was then applied to a municipal wastewater collected in a real wastewater treatment plant, containing at least 9 xenobiotics as drugs, pesticides, plasticizers and personal care products. Although the harsh chemical and biological conditions of the effluent influenced enzyme stability, the reaction took place, and above 70% transformation was obtained for most analytes during the 24 h experiment. Bioassays were carried out to estimate the estrogenic activity (the E-screen test and the MELN gene-reporter luciferase assay) and the ecotoxicity (Lepidium sativum, Pseudokirchneriella subcapitata and Vibrio fischeri), demonstrating the capability of laccases to mediate an effective detoxification of the wastewater and a decrease of the estrogenic activity. treatment plant; U/L, enzymatic activity expressed as International Unit per liter; PR, percentage of removal; MEC, minimal effective concentration; t 1/2 , analyte half-life; Ti, Target Ions; RSD%, relative standard deviation percentage; LOQ, limit of quantification; QCx, quality control samples; Rel.Err %, relative error percentage; COD, chemical oxygen demand; GI%, germination index; I%, inhibition percentage of the algal growth or of the bacterial bioluminescence; EEQ, 17-b-estradiol equivalent quantity; RPE %, relative proliferative effect; TRANS%, rate of luciferase gene expression.

show abstract

Section: Minimal Effective Concentration (Mec)mentioning

confidence: 85%

Section: Minimal Effective Concentration (Mec)mentioning

confidence: 98%

Removal of micropollutants by fungal laccases in model solution and municipal wastewater: evaluation of estrogenic activity and ecotoxicity

Spina

Cordero

Schilirò

et al. 2015

Journal of Cleaner Production

View full text Add to dashboard Cite

show abstract

“…Its use for indigo dye oxidation in denim cloth has also been reported (Riva 2006). On the other hand, enzymatic transformation of polyaromatic hydrocarbons Thongkred et al 2011;Niu et al 2013), phenolic compounds (Ullah et al 2000;Gaitan et al 2011), pesticides (Maruyama et al 2006;Torres-Duarte et al 2009), endocrine-disrupting compounds (Cabana et al 2007;Torres-Duarte et al 2012), PCBs (Gayosso-Canales et al 2012, pharmaceuticals (Touahar et al 2014), industrial dyes (Rodriguez et al 1999;Wesenberg et al 2003), and cleaning of certain explosives from the soil such as trinitrotoluene (Shraddha et al 2011) by laccase make it an enzyme with enormous potential for remediation processes of polluted sites and elimination of environmental threats. Laccases are considered potential biocatalysts for large-scale transformation of xenobiotic compounds because they do not require exogenous co-factors (Martins et al 2015) and have comparatively high stability (Loera Corral et al 2006) against temperature and pH.…”

Section: Introductionmentioning

confidence: 94%

Laccase encapsulation in chitosan nanoparticles enhances the protein stability against microbial degradation

Koyani

Vázquez-Duhalt

2016

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

A novel concept with the result of enzyme stabilization against microbial degradation in real bioremediation processes was developed through the encapsulation of laccase in chitosan nanoparticles. Besides of abundant information on laccase-chitosan conjugates, we report the laccase encapsulation into nanoparticles based in chitosan. The chitosan-tripolyphosphate technique was applied for the production of morphologically homogeneous enzymatic nanoparticles, with high enzyme encapsulation efficiency, small asymmetric sizes (from 40 to 90 nm), and rough surfaces. Contrary to macroscopic immobilized enzymes, temperature and pH activity profiles of nano-sized laccase were similar to those of free enzyme. The substrate affinity constant (K M) of nano-encapsulated laccase was similar to these from free enzyme, while its activity rate constant (k cat) represented 60 % of these obtained with free enzyme. Importantly, stability of nano-encapsulated laccase against microbial degradation in soil, compost, and wastewater was significantly increased. After 24 h exposure to wastewater from a treatment plant, the laccase activity of the nanoparticles was 82.8 % of initial activity, compared with only 7.8 % retained activity for free enzyme. After 36 h incubation in compost extract, the laccase nanoparticles showed 72.4 % of the initial activity, while the free enzyme was almost completely inactivated. Finally, after 84 h incubation in soil extract, the nanoparticles and free preparations showed 57.9 and 17.3 % of the initial activity, respectively. Thus, the nanoencapsulation of enzymes able to transform pollutants is an alternative to improve the operational lifetime of enzymes in real environmental applications.

show abstract

“…This phenomenon was true for very recalcitrant compounds as the herbicide dymron (Maruyama et al 2006) and halogenated pesticides (Torres-Duarte et al 2009). …”

Section: Laccase Mediated Systemmentioning

confidence: 99%