Hairy root formation in peanut by the wild type strains of Agrobacterium rhizogenes.

Genetic Transformation in Vaccaria pyramidata Using Agrobacterium rhizogenes.

“…Leaves were excised at their base from the seedlings and cut in lengths of about 5 mm. Leaf pieces were immersed in a suspension culture of a mikimopine type strain A13 of A. rhizogenes (Daimon et al, 1990) for 10 min, and blotted on a sterilized filter paper to remove any excessive suspension. Then, the pieces were put onto a half-strength MS medium (Murashige and Skoog, 1962) solidified with 2% gellan gum (Wako Pure Chem.…”

Agrobacterium rhizogenes-Mediated Transformation of Plants

“…Agropine Lippincott and Lippincott (1969); White and Nester (1980); Petit et al (1983 Mikimopine Daimon et al (1990) RIOOO Park and Facchini (2000) nism by which the rol gene products cause the hairy root phenotype is still not completely understood. In addition to the rol genes, the Ri T-DNA also contains genes for opine biosynthesis.…”

Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro

“…Cotyledons and hypocotyls of the seedlings thus obtained were used as explants. A mikimopine producing (Isogai et al 1988) wild-type strain of Agrobacterium rhizogenes, A13 (MAFF-02-10266; Daimon et al 1990), was used for the infection. The bacterial culture was prepared by culturing loopful bacteria in 30 ml of liquid YEP (Chilton et al 1974) medium for 12 h at 160 revs min −1 at 28°C in the dark.…”