Hair Regeneration Therapy: Application of Adipose-Derived Stem Cells

Fukuoka, Hirotaro; Narita, Keigo; Suga, Hirotaka

doi:10.2174/1574888x12666170522114307

Cited by 68 publications

(54 citation statements)

References 16 publications

(15 reference statements)

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“…The mean increase in the number of hairs in men and women was 29±4.1 and 15.6±4.2, respectively, and no significant difference was detected between the finasteride and no finasteride group and the number of hairs was significantly higher in the treated group compared to placebo. In another study by Fukuoka et al in 2017, they evaluated the effect of the same medium in 21 patients (16 men and 5 women) in the same way 23. It was informed that the number of hairs increased from baseline of 141.2±31.4 in men and 109.8±43.5 in women after 3 months.…”

Section: Discussionmentioning

confidence: 99%

<p>Autologous adipose transplantation an effective method to treat alopecia after trauma: a case report</p>

Nilforoushzadeh

Lotfi

Heidari‐Kharaji

2019

CCID

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Finding new therapies for male and female pattern hair loss treatment remains as great interest. The autologous fat grafting technique is a new method, and clinical experience was increased over the past 10–15 years. Adipose tissue is a biologically active and important tissue and can help drive the complex hair growth cycle. Surgeons have previously reported that autologous fat not only have a positive affect for reconstructive volume and esthetics after transplant but can also have positive skin and hair changes post-transplantation. In this study, lipoaspiration of adipose was performed by cannula and scalp injection was done. In this case report, the authors report that scalp injection of adipose tissue has a positive effect on a patient with alopecia after trauma. The findings suggest adipose tissue can be a promising alternative method to treating men and women alopecia.

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Section: Discussionmentioning

confidence: 99%

<p>Autologous adipose transplantation an effective method to treat alopecia after trauma: a case report</p>

Nilforoushzadeh

Lotfi

Heidari‐Kharaji

2019

CCID

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“…AD-SVFs-conditioned medium (AD-SVFs-CM) was used for patients with HL in an examination by Fukuoka and Suga [63]. A commercial product containing a protein arrangement with AD-SVFs was used (AAPE, Prostemics, Seoul, Korea), which had different growth factors (HGF, FGF-I, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor (GM-CSF), IL-6, VEGF, and TGF).…”

Section: Clinical Intra-surgical Applications Of Ad-svfs In Hair Lmentioning

confidence: 99%

“…The treatment (0.02 mL/cm 2 of the arrangement) was performed by injecting the suspension intradermal every three to five weeks (for four to six sessions), and hair growth was checked using trichograms. A real enhancement in hair thickness was accomplished in patients of both sexes [63]. Shin et al [64] used AD-SVFs and the conditioned media of AD-SVFs (AD-SVFs-CM) in an observational examination of 27 women with female pattern hair loss (FPHL).…”

Section: Clinical Intra-surgical Applications Of Ad-svfs In Hair Lmentioning

confidence: 99%

Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

Gentile

Garcovich

2019

Cells

211

170

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The use of stem cells has been reported to improve hair regrowth in several therapeutic strategies, including reversing the pathological mechanisms, that contribute to hair loss, regeneration of hair follicles, or creating hair using the tissue-engineering approach. Although various promising stem cell approaches are progressing via pre-clinical models to clinical trials, intraoperative stem cell treatments with a one-step procedure offer a quicker result by incorporating an autologous cell source without manipulation, which may be injected by surgeons through a well-established clinical practice. Many authors have concentrated on adipose-derived stromal vascular cells due to their ability to separate into numerous cell genealogies, platelet-rich plasma for its ability to enhance cell multiplication and neo-angiogenesis, as well as human follicle mesenchymal stem cells. In this paper, the significant improvements in intraoperative stem cell approaches, from in vivo models to clinical investigations, are reviewed. The potential regenerative instruments and functions of various cell populaces in the hair regrowth process are discussed. The addition of Wnt signaling in dermal papilla cells is considered a key factor in stimulating hair growth. Mesenchymal stem cell-derived signaling and growth factors obtained by platelets influence hair growth through cellular proliferation to prolong the anagen phase (FGF-7), induce cell growth (ERK activation), stimulate hair follicle development (β-catenin), and suppress apoptotic cues (Bcl-2 release and Akt activation).

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“…To develop CM formulation translatable to clinical use, understanding of the effects of the manufacturing process on the product cost, logistics of storage and handling, and bioactivity is critically important. That is why, even though preclinical ( Bi et al, 2007 ; Yamagata et al, 2013 ; Suto et al, 2016 ) and some clinical ( Zhou et al, 2013 ; Dahbour et al, 2017 ) studies show the ability of unconcentrated CM to exert beneficial effects and limit pathological conditions, significant effort is devoted to analyze the effects of CM lyophilizates ( Fukuoka et al, 2017 ; Katagiri et al, 2017 ) and EV concentrates ( Kordelas et al, 2014 ; Nassar et al, 2016 ). Formulation of the particular therapeutic is likely to be optimized for specific clinical use; therefore, elucidation of the nature of bioactive factors is of utmost importance for the preservation of therapeutic potential during the manufacturing process and will be of utmost significance to gaining regulatory approval.…”

Section: Discussionmentioning

confidence: 99%

Distinct Factors Secreted by Adipose Stromal Cells Protect the Endothelium From Barrier Dysfunction and Apoptosis

Lü

Merfeld-Clauss

Jawed

et al. 2020

Front. Cell Dev. Biol.

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We have shown previously that adipose stromal cell (ASC)-derived conditioned media (CM) limited lung injury, endothelial barrier dysfunction, and apoptosis. Here, we used endothelial hyperpermeability and apoptosis assays to investigate how concentration processes affect endothelium-directed bioactivity of ASC-CM and to gain information on the nature of bioactive factors. Comparison of ASC-CM concentrated with differential molecular weight (MW) cutoff filters showed that endothelial barrier protection depended on the species-specific factors in ASC-CM fractionated with MW > 50 kDa. Known barrier regulators-keratin growth factor (KGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF)-were detected in ASC-CM fraction of > 100 kDa. Pretreatment of endothelial monolayers with concentrations of KGF, VEGF, and HGF detected in ASC-CM showed that only KGF and HGF protect the endothelium from barrier dysfunction. Depletion of KGF and HGF from ASC-CM attenuated ASC-CM's ability to protect the endothelial barrier. In contrast to barrierprotective factors, apoptosis-protective factors fractionated with MW < 3 kDa and were not species-specific. Application of donors of apoptosis-mitigating gases showed that the CO donor carbon monoxide-releasing molecule 2 (CORM2) protected the endothelium from apoptosis, while the H 2 S donor NaSH did not. Knockdown of COgenerating heme oxygenase 1 in ASC attenuated ASC-CM's ability to protect the endothelium from apoptosis. We have shown that tumor necrosis factor alpha (TNFα)induced apoptosis in endothelium is c-Jun N-terminal kinase (JNK)-dependent, and JNK activation is inhibited by ASC-CM pretreatment of endothelial cells. ASC-CM from heme oxygenase 1-depleted ASC displayed attenuated ability to suppress endothelial JNK activation, suggesting that CO-mediated protection of the endothelium from apoptosis is achieved by the downregulation of the JNK pathway. Altogether, our results demonstrate that the concentration of ASC-CM with low MW cutoff filters significantly reduces its anti-apoptotic activity while preserving its barrier-protective activity.

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Hair Regeneration Therapy: Application of Adipose-Derived Stem Cells

Abstract: Treatment using adipose-derived stem cell-conditioned medium is highly effective and may represent a new therapy for alopecia.

Cited by 68 publications

References 16 publications

<p>Autologous adipose transplantation an effective method to treat alopecia after trauma: a case report</p>

<p>Autologous adipose transplantation an effective method to treat alopecia after trauma: a case report</p>

Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

Distinct Factors Secreted by Adipose Stromal Cells Protect the Endothelium From Barrier Dysfunction and Apoptosis

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