Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis.

Rathlev, Tara

doi:10.1136/sti.43.3.181

Cited by 54 publications

(32 citation statements)

References 8 publications

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“…Die Wassermannsche Komplementbindungsreaktion stellte während Jahr zehnten die Referenzreakion dar, an der alle andern Neuentwicklungen, mehr als 200 an der Zahl, gemessen wurden. Wassermann et al [1906] Rathlev [1967] nicht an. Es werden spezifische Immunglobuline der Gund M-Klasse nachgewiesen, die gegen spezies-und gattungsspezifische Proteinanteile von T. pallidum gerichtet sind.…”

Section: Historischer Rückblickunclassified

Lues-Serologie – gestern, heute und morgen

Rufli¹

1982

Dermatology

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After an historical survey, the various modern serologic tests for syphilis are dealt with. The humoral immunological phenomena, which develop in a certain chronological order, antilipoidal (non-specific) and antitreponemal (specific) antibodies of the classes IgM and IgG are presented. Particularly the demonstration of specific antitreponemal antibodies of the IgM class has opened up new perspectives in the serologic diagnostics of syphilis. For the present, screening examinations and the confirmation of the diagnosis are the domain of the specific tests (TPHA, FTA), while for the assessment of the therapy effect and of reinfection the unspecific tests (VDRL) are still to be employed.

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Section: Historischer Rückblickunclassified

Lues-Serologie – gestern, heute und morgen

Rufli¹

1982

Dermatology

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“…In countries such as the USA, non-treponemal tests are recommended for screening and tests such as the RPR, and are confirmed with a standard treponemal test such as the fluorescent treponemal antibody absorption (FTA-ABS) test or the TPHA, both of which are based on extracts of Nichols strain to detect antibodies directed against treponemal components. 2,7,11,12 Recently, several enzyme immunoassays (EIAs), some of which are based on specific T. pallidum recombinant antigens, 9,13,14 have been developed and evaluated as treponemal tests for syphilis. 12,[15][16][17][18][19] The Serodia T. pallidum particle agglutination (TPPA) test (Fujirebio Inc., Tokyo, Japan) is an alternative to the TPHA and is manufactured using gelatin particle carriers sensitized with purified pathogenic T. pallidum (Nichols strain), to detect antibodies directed against T. pallidum antigens in serum or plasma specimens.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Serodia Treponema pallidum particle agglutination, the Murex Syphilis ICE and the Enzywell TP tests for serodiagnosis of syphilis

et al. 2005

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We evaluated the Treponema pallidum haemagglutination assay (TPHA), a treponemal test, with three other treponemal tests, the Serodia T. pallidum particle agglutination assay, the Murex Syphilis ICE IgG + IgM enzyme immunoassay (EIA) and the Enzywell TP IgG + M EIA (a new rapid EIA) for use in conjunction with the rapid plasma reagin test (RPR), a non-treponemal test, for serodiagnosis of syphilis. In all, 124 serum samples were found reactive with RPR and/or TPHA after testing by the routine laboratory protocol. Twenty-three (18.5%) of them were positive only by RPR test and were evaluated as biologically false-positive, 16 were positive only by the TPHA and 84 by both the RPR and TPHA tests; one sample was non-specific (heterophile reaction) in the TPHA. Agreements of the TPHA with the Serodia TPPA, the Murex Syphilis ICE and the Enzywell TP tests were 96.7%, 100% and 99.1%, respectively. We conclude that each one of the tests, the Serodia TPPA, the Murex Syphilis ICE and the Enzywell TP, is an appropriate substitute for screening for serodiagnosis of syphilis.

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“…It is technically simpler test and detects reactive antibody that agglutinates red blood cells sensitized with T.pallidum antigen (Rathlev, 1967;Rudolph, 1976;Tomizawa, 1966). A formalinized, tanned sheep (or turkey) erythrocytes are sensitized with ultrasonicated antigen from T.pallidum, Nichols strain.…”

Section: Tpha and Tppamentioning

confidence: 99%

Laboratorial Diagnosis of Syphilis

Sato¹

2011

Syphilis - Recognition, Description and Diagnosis

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Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis.

Cited by 54 publications

References 8 publications

Lues-Serologie – gestern, heute und morgen

Lues-Serologie – gestern, heute und morgen

Evaluation of the Serodia Treponema pallidum particle agglutination, the Murex Syphilis ICE and the Enzywell TP tests for serodiagnosis of syphilis

Laboratorial Diagnosis of Syphilis

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