Habitat degradation impacts black howler monkey (<i>Alouatta pigra</i>) gastrointestinal microbiomes

Amato, Katherine R.; Yeoman, Carl J.; Kent, Angela D.; Righini, Nicoletta; Carbonero, Franck; Estrada, Alejandro; Gaskins, H. Rex; Stumpf, Rebecca M.; Yıldırım, Süleyman; Torralba, Manolito; Gillis, Marcus; Wilson, Brenda A.; Nelson, Karen E.; White, Bryan A.; Leigh, Steven R.

doi:10.1038/ismej.2013.16

Cited by 1,030 publications

(849 citation statements)

References 68 publications

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“…Three of these enriched genera are related with short-chain fatty acids (SCFAs) production. Butyricimonas, a butyrate producer (23), and Prevotella, a propionate producer (24), increased the relative abundance more dramatically in the ProPre group than in the other groups. The relative abundance of Alistipes, a major SCFAs producer in gut (25), decreased in the control group but increased in the ProPre and ProTreat groups by 32% and 29%, respectively.…”

Section: Probiotics Mediate the Structural And Functional Compositionmentioning

confidence: 90%

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

Sung

Lee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

486

386

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The beneficial roles of probiotics in lowering the gastrointestinal inflammation and preventing colorectal cancer have been frequently demonstrated, but their immunomodulatory effects and mechanism in suppressing the growth of extraintestinal tumors remain unexplored. Here, we adopted a mouse model and metagenome sequencing to investigate the efficacy of probiotic feeding in controlling s.c. hepatocellular carcinoma (HCC) and the underlying mechanism suppressing the tumor progression. Our result demonstrated that Prohep, a novel probiotic mixture, slows down the tumor growth significantly and reduces the tumor size and weight by 40% compared with the control. From a mechanistic point of view the down-regulated IL-17 cytokine and its major producer Th17 cells, whose levels decreased drastically, played critical roles in tumor reduction upon probiotics feeding. Cell staining illustrated that the reduced Th17 cells in the tumor of the probiotictreated group is mainly caused by the reduced frequency of migratory Th17 cells from the intestine and peripheral blood. In addition, shotgun-metagenome sequencing revealed the crosstalk between gut microbial metabolites and the HCC development. Probiotics shifted the gut microbial community toward certain beneficial bacteria, including Prevotella and Oscillibacter, that are known producers of antiinflammatory metabolites, which subsequently reduced the Th17 polarization and promoted the differentiation of antiinflammatory Treg/Tr1 cells in the gut. Overall, our study offers novel insights into the mechanism by which probiotic treatment modulates the microbiota and influences the regulation of the T-cell differentiation in the gut, which in turn alters the level of the proinflammatory cytokines in the extraintestinal tumor microenvironment.H epatocellular carcinoma (HCC) is one of the most common cancers, the sixth most common neoplasm, and the second most deadly type of cancer worldwide (1). The traditional HCC treatment, including surgical treatment, local ablation therapy, and chemotherapy, could offer potential cure, yet patients are facing many limitations including the poor hepatic reserve. HCC is clearly a disease for which alternative therapeutic strategies must be developed. A better understanding of the interactions between cancer cells and stromal components in the tumorassociated proinflammatory microenvironment would be important for the management of this disease.The tumor microenvironment is infiltrated with various immune cells such as T cells, macrophages, neutrophils, natural killer (NK) cells, and myeloid-derived suppressor cells. Inflammation is known to play a pivotal role in tumor development by escalating tumor angiogenesis and cell growth. Once a solid tumor is formed, inflammation arises in the tumor-promoting direction. At the same time, new vasculature is needed in the tumor to provide nutrients and oxygen to support the growth of cancer cells, and this process plays a critical role in HCC, a highly vascularized tumor (2). Inflammation and angiogene...

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Section: Probiotics Mediate the Structural And Functional Compositionmentioning

confidence: 90%

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

Sung

Lee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

486

386

View full text Add to dashboard Cite

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“…The taxonomy of each 16S rRNA gene sequence was analyzed using the RDP Classifier (http://rdp.cme.msu.edu/) against the silva (SSU115)16S rRNA database by applying a confidence threshold of 70% (Amato et al, 2013). The relationships between environmental variables and the cloud water bacterial community were analyzed using redundancy analysis (RDA) with CANOCO software (version 4.56, Microcomputer Power, Inc., Ithaca, NY, USA).…”

Section: Illumina Miseq Sequencing and Processing Of Sequencing Datamentioning

confidence: 99%

Chemical Composition and Bacterial Community in Size-Resolved Cloud Water at the Summit of Mt. Tai, China

Zhu

Chen

Wang

et al. 2018

Aerosol Air Qual. Res.

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A three-stage collector was used to collect size-resolved cloud samples at the summit of Mt. Tai. Subsequently, analyses of pH values, water-soluble ions, and trace metals were performed and bacterial community were conducted using MiSeq amplicon sequencing. The pH values of the samples decreased as droplet sizes decreased. Sulfate (SO 4 2-), nitrate (NO 3 -) and ammonium (NH 4 + ) were the main secondary inorganic ions which their concentration distributed significantly different from size-resolved cloud water. The NH 4 + concentration was higher in smaller droplets. The SO 4 2-and NO 3 -concentrations were higher in larger droplets. The Ca 2+ concentration increased as droplet size increased. Small droplet samples tended to have lower pH value, mainly because of the more acidic (SO 4 2-and NO 3 -) and less acid-neutralizing (NH 4 + and Ca 2+ ) components. The bacterial community in size-resolved cloud samples were firstly recognized and dominated by the genera of Lactococcus (average abundance 34.9%) and Bacillus (average abundance 34.0%). Linear discriminant analysis effect size (LEfSe) revealed differences of the bacterial community in size-resolved cloud water samples, which was probably caused by the bacterial size. Redundancy analysis suggested several minor correlations that the H 2 O 2 , NO 2 -concentrations, and trace metals exert effects on the bacterial community.

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“…Purified amplicons were pooled in equimolar and pairedend sequenced (2 × 250) on an MiSeq according to the standard protocols (Amato et al, 2013).…”

Section: Dna Extraction and High-throughput Sequencing Of Itsmentioning

confidence: 99%

Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing

Agusman

Feng

2017

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Since algae are well known as the host of fungi, the information about total fungal community associated with alga is needed to give the initial data that is useful for natural product discovery. The study on total fungal community associated with marine macroalga U. intestinalis from Xiamen-China was carried out using MiSeq, a powerful platform for microbial ecology studies. Metagenomics DNA was isolated from surfaces of the U. intestinalis thallus and rocks colonized by this alga and 18S ribosomal RNA region was sequenced using MiSeq Illumina to yield 81360 sequences from 162720 reads, which average read length is 245.61 bp. The sequences were assigned to operational taxonomic units (OTUs), where 101 genera represent 5 phyla, 17 classes and 64 families of fungal. Ascomycota was the highest abundance share of fungal community at phylum level. Meanwhile Aspergillus, Blastobotrys, Alternaria, Knufia, and Fusarium were some of dominant genera found associated with U. intestinalis which have been reported as the promising source of natural products. By using MiSeq platform, this study revealed the total community structure of algicolous fungi associated with U. intestinalis.

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Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes

Cited by 1,030 publications

References 68 publications

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

Probiotics modulated gut microbiota suppresses hepatocellular carcinoma growth in mice

Chemical Composition and Bacterial Community in Size-Resolved Cloud Water at the Summit of Mt. Tai, China

Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing

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