2018
DOI: 10.18632/aging.101572
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Abstract: Methylation of histones H4 at lysine 20 position (H4K20me), which is functional in DNA repair, represents a binding site for the 53BP1 protein. Here, we show a radiation-induced increase in the level of H4K20me3 while the levels of H4K20me1 and H4K20me2 remained intact. H4K20me3 was significantly pronounced at DNA lesions in only the G1 phase of the cycle, while this histone mark was reduced in very late S and G2 phases when PCNA was recruited to locally micro-irradiated chromatin. H4K20me3 was diminished in l… Show more

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Cited by 28 publications
(25 citation statements)
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References 45 publications
(71 reference statements)
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“…Since monomethylation of H3K27, H3K79, and H4K20 are all linked to gene activation, whereas tri-methylation of H3K27 and H3K79 are linked to repression [54], these ndings may provide additional insight into the mechanism by which BRDi alters tumorigenic gene expression in DIPG. H4K20me, which is functional in DNA repair, represents a binding site for the 53BP1 protein; H3K9me3 and H4K20me3 represent epigenetic markers important to the function of 53BP1 in non-homologous end joining (NHEJ) repair [55]. As such, additional studies of how JQ1 may augment response to RT are necessary.…”
Section: Discussionmentioning
confidence: 99%
“…Since monomethylation of H3K27, H3K79, and H4K20 are all linked to gene activation, whereas tri-methylation of H3K27 and H3K79 are linked to repression [54], these ndings may provide additional insight into the mechanism by which BRDi alters tumorigenic gene expression in DIPG. H4K20me, which is functional in DNA repair, represents a binding site for the 53BP1 protein; H3K9me3 and H4K20me3 represent epigenetic markers important to the function of 53BP1 in non-homologous end joining (NHEJ) repair [55]. As such, additional studies of how JQ1 may augment response to RT are necessary.…”
Section: Discussionmentioning
confidence: 99%
“…Since monomethylation of H3K27, H3K79, and H4K20 are all linked to gene activation, whereas tri-methylation of H3K27 and H3K79 are linked to repression [54], these ndings may provide additional insight into the mechanism by which BRDi alters tumorigenic gene expression in DIPG. H4K20me, which is functional in DNA repair, represents a binding site for the 53BP1 protein; H3K9me3 and H4K20me3 represent epigenetic markers important to the function of 53BP1 in non-homologous end joining (NHEJ) repair [55].…”
Section: Discussionmentioning
confidence: 99%
“…Immunofluorescence was performed following [47]. The cells were fixed in 4% paraformaldehyde (PFA) for 10 min at room temperature (RT), permeabilized with 0.2% Triton X-100 (Merck) for 15 min (Merck), and washed twice in phosphate-buffered saline (PBS) for 10 min.…”
Section: Immunofluorescence Stainingmentioning
confidence: 99%
“…Western blotting was performed using the methods reported by [47]. We used the following primary antibodies: anti-phosphorylated histone H2AX (γH2AX; phospho S139; #ab2893, Abcam), anti-FTO (#ab92821, Abcam), anti-α-tubulin (#ab80779 Abcam), anti-DNMT1 (#ab188453 Abcam), anti-H3 (#ab7091, Abcam), anti-H3K9me3 (#ab8898, Abcam), anti-METTL3 (#A8370, Abclonal), anti-METTL14 (#A8530, Abclonal), and anti-METTL16 (#HPA020352, Atlas Antibodies).…”
Section: Western Blottingmentioning
confidence: 99%
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