2011
DOI: 10.1021/pr200705z
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Abstract: Ferritin, the major intracellular iron-storage protein, is made of 24 subunits of two types, H and L. Besides regulating intracellular iron homeostasis, it has been found that ferritin, in particular the H subunit (FHC), is involved in different biological events such as cell differentiation and pathologic states (i.e., neurodegeneration and cancer). This study is aimed at investigating the whole-cell proteome of FHC-expressing and sh-RNA-silenced human metastatic melanoma cells (MM07(m)) in the attempt to ide… Show more

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Cited by 24 publications
(29 citation statements)
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“…In the last years we and others have highlighted the role of ferritin heavy chain in the process of neoplastic transformation. We have documented that the modulation of FHC content determines, in different cell types, a severe alteration of gene expression, as evaluated by proteomic [ 29 ] and transcriptomic analysis [ 34 ]. In human metastatic melanoma cells this phenomenon is accompanied by a reduced invasiveness and adhesion along with a strong decrease in growth activity [ 29 ].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In the last years we and others have highlighted the role of ferritin heavy chain in the process of neoplastic transformation. We have documented that the modulation of FHC content determines, in different cell types, a severe alteration of gene expression, as evaluated by proteomic [ 29 ] and transcriptomic analysis [ 34 ]. In human metastatic melanoma cells this phenomenon is accompanied by a reduced invasiveness and adhesion along with a strong decrease in growth activity [ 29 ].…”
Section: Discussionmentioning
confidence: 99%
“…Lentiviral preparation and transduction were performed as described in Di Sanzo et al [ 29 ]. Cells were stably transduced with a lentiviral DNA containing either an shRNA that targets the 196–210 region of the FHC mRNA (sh29432) (MCF-7 shFHC ) (H460 shFHC ) or a control shRNA without significant homology to known human mRNAs (MCF-7 shRNA ) (H460 shRNA ).…”
Section: Methodsmentioning
confidence: 99%
“…The multi-functional role of ferritin becomes increasingly evident by modifying its expression. In two separate studies downregulation of FTL with an antisense construct (59) and FTH1 with shRNA (119) in melanoma cells inhibited proliferation and invasion in vitro and tumor growth in vivo (59). In melanoma cells FTL is necessary to resist oxidative stress-induced apoptosis (59).…”
Section: Iron Storagementioning
confidence: 99%
“…A deep survey of the literature highlights that the link between ferritin and cancer cell growth is very complex and often conflicting since it is exerted in a cell-specific manner and through different mechanisms. In human metastatic melanoma MM07 cells and in erytholeukemia K562 cells, FHC-silencing is accompanied by decreased growth activity through the substantial modification of a repertoire of transcripts, miRNAs and proteins expression [32,33]. In particular, in K562 cells, the FHC-dependent modulation of miR-125b affects RAF1/pERK1/2 expression leading to a reduced proliferation rate in the FHC-silenced cells [33].…”
Section: Ferritin and Cancer Cell Growthmentioning
confidence: 99%