Gynogenesis and sex determination in shortnose sturgeon, Acipenser brevirostrum Lesuere

Flynn, Shawn R.; Matsuoka, Makoto; Reith, Michael; Martin-Robichaud, D. J.; Benfey, Tillmann J.

doi:10.1016/j.aquaculture.2005.09.016

Cited by 84 publications

(61 citation statements)

References 12 publications

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“…Despite quite contradictory results of sturgeon sex determination system assessment, female heterogamety is commonly accepted (Keyvanshookoh and Gharaei 2010). In accordance with this hypothesis, high percentage of females in gynogenetic progeny was shown in a number of sturgeon species: Acipenser transmontanus 82% (Van Eenennaam 1999), Acipenser baerii 81% (Fopp-Bayat 2010), Polyodon spathula 80% (Shelton and Mims 2012), bester 70-80% (Omoto et al 2005), Acipenser brevirostrum 65% (Flynn et al 2006). Furthermore, the other applications of gynogenesis are related to the interesting ploidy level system of sturgeon and its evolution.…”

Section: Introductionmentioning

confidence: 49%

Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

Lebeda¹,

Gazo²,

Flajšhans³

2014

CZECH J ANIM SCI

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Chromosomal manipulations in sturgeons, particularly gynogenesis, are interesting due to the potential to change female ratio in progeny that can be useful for caviar production. The optimization of UV treatment for induction of gynogenesis is complicated due to high and variable optical density of the milt due to differential spermatozoa concentration, and because of sensitivity of spermatozoa's motility apparatus. Therefore in this study we compared chemical methods of sperm treatment as an alternative to short wave-length UV treatment; evaluation considers impact on spermatozoa motility, DNA integrity, and efficiency of DNA inactivation. Dimethyl sulfate (DMS) in concentrations of 2.5-30mM was applied to spermatozoa in order to inactivate DNA. Also ethidium bromide (EB), psoralen (PS), and 4'-aminomethyl-4,5' ,8-trimethylpsoralen (AMT) were used to increase sensitivity of spermatozoa's DNA to long wavelength UV-A light (360 nm). CASA analyses of treated sperm showed strong negative effects on spermatozoa motility with the increasing concentration of active substances. Additionally in case of PS, EB, and DMS treatment comet assay did not reveal significant DNA damage of sperm at the range of concentrations relatively safe for spermatozoa motility. Flow cytometric analysis of relative DNA content in larvae resulting from activation of normal ova of sterlet with the treated sperm showed low efficiency of haploid gynogenesis induction. The putative gynogenetic larvae were found after treatment with PS in concentrations higher than 18µM and EB higher than 10µM followed by UV-A irradiation at the dose of 900 J/m 2 and DMS up to 5mM. Because of an overwhelming impact on the sperm motility and relatively low DNA damage, treatment of sperm with PS, EB or DMS did not prove efficient compared with a widely used UV-C irradiation treatment. In contrast, treatment with AMT followed by UV-A showed lower influence on spermatozoa motility and higher efficiency of DNA damaging resulting in the higher percentage of gynogenotes in the progeny, thus could be considered as a possible substitution for UV-C treatment.

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Section: Introductionmentioning

confidence: 49%

Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

Lebeda¹,

Gazo²,

Flajšhans³

2014

CZECH J ANIM SCI

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“…1, 2). The initiation time of shock is affected by many factors and differs in various fish species, such as 2-4 min after fertilization for Sarpunti Puntius sarana (Hamilton) (Chakraborty et al 2006), 6.5 min for turbot Scophthalmus maximus (Piferrer et al 2004), and 20 min for shortnose sturgeon Acipenser brevirostrum (Lesueur) (Flynn et al 2006). As a result, optimization of the timing of shock application is critically important element in gynogenesis induction.…”

Section: Discussionmentioning

confidence: 98%

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Chen

Yang

et al. 2010

Aquacult Int

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In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30-50 mJ cm -2 ) were cold shocked in sea water of -1°C for 40-75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female, which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that the average H O was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity in gynogenetic population.

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“…Thus, a second step is needed to re-establish diploidy in the eggs by blocking the extrusion of the second polar body (meiotic gynogenesis) or by preventing the first embryonic cleavage (mitotic gynogenesis) by an external shock (thermal, pressure, or chemical) (Devlin and Nagahama 2002). Currently, gynogenesis has been induced successfully in many species including in turbot (Yamamoto 1999;Felip et al 2001;Piferrer et al 2004;Luckenbach et al 2004;Flynn et al 2006;Morgan et al 2006). However, as far as we know, the successful production of gynogenetic diploid turbot with heterologous sperm has not yet been reported.…”

Section: Introductionmentioning

confidence: 96%

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

You

Sun

et al. 2008

Aquacult Int

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Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig's effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm -2 . At 15°C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3°C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1°C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UVirradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring.

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Gynogenesis and sex determination in shortnose sturgeon, Acipenser brevirostrum Lesuere

Cited by 84 publications

References 12 publications

Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

Chemical induction of haploid gynogenesis in sterlet Acipenser ruthenus

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

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