Gut microbiota changes in patients with autism spectrum disorders

Ding, Xiuhua; Xu, Yiran; Zhang, Xiaoli; Zhang, Lingling; Duan, Guiqin; Song, Ci; Li, Zhenghua; Yang, Yanyan; Wang, Yazhe; Wang, Xiaoyang; Zhu, Changlian

doi:10.1016/j.jpsychires.2020.06.032

Cited by 105 publications

(128 citation statements)

References 58 publications

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“…Obviously, the result of this study revealed the differences in the trend of gut bacterial species abundance distribution, which was affected by both the ASD and the nanoparticles. However, the conflict results were reported in various studies, for example, Ding et al [ 31 ] found that there was a significant increase in the relative abundance of unidentified Lachnospiraceae , Clostridiales , Erysipelotrichaceae , Dorea , Collinsella and Lachnoclostridium , while Bacteroides , Faecalibacterium , Parasutterella and Paraprevotella were significantly lower in the ASD group than in the control group. Bezawada et al [ 8 ] revealed that species reported to be significantly higher in abundance in autistic children included Clostridium , Sutterella , Desulfovibrio and Lactobacillus .…”

Section: Resultsmentioning

confidence: 90%

“…The latest research in childhood neurodevelopmental disorders, including ASD, has transferred from genetics to environmental factors in the gut microbiome, which plays an important role [ 1 , 28 , 29 ]. Indeed, many studies have described the changes of intestinal microbiota of ASD children using non-culture-based methods [ 30 , 31 , 32 ]. This study investigated and compared the number, diversity, and community structure of gut bacteria from the fecal samples between healthy and ASD children using both the traditional plate counting method and 16S rRNA gene amplicon sequencing approaches.…”

Section: Resultsmentioning

confidence: 99%

“…In order to open the possibility for new potential strategies in this disease, it is very necessary to identify ASD-associated gut bacteria, however, very limited scientific investigation into the potential biological connection between the ASD and gut bacteria. In particular, different studies often showed the conflicted results in this field, for example, Ding et al [ 31 ] revealed that the presence of unidentified Faecalibacterium , Erysipelotrichaceae , and Lachnospiraceae was correlated with the severity of ASD. Ahmed et al [ 43 ] found that there was no correlation between the severity of autism symptoms and the altered gut microbiome, although evidence of changes was revealed in the gut microbiome of autistic children compared to the unrelated control.…”

Section: Resultsmentioning

confidence: 99%

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Impact of Zinc Oxide Nanoparticles on the Composition of Gut Microbiota in Healthy and Autism Spectrum Disorder Children

Jiang

Zhou

et al. 2021

Materials

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Autism spectrum disorder (ASD) seriously affects children’s health, while the gut microbiome has been widely hypothesized to be involved in the regulation of ASD behavior. This study investigated and compared the number, diversity, and population structure of gut microbiota between healthy and ASD children and their susceptibility to zinc oxide nanoparticles (ZnONPs) based on the measurement of live cell number, living/dead bacterial staining test, flow cytometry observation and bacterial community analysis using 16S rRNA gene amplicon sequencing. The result of this present study revealed that ASD children not only significantly reduced the live cell number and the community diversity of gut bacteria, but also changed the gut bacterial community composition compared to the healthy children. In addition, this result revealed that ZnONPs significantly reduced the number of live bacterial cells in the gut of healthy children, but not in that of ASD children. In contrast, ZnONPs generally increased the gut bacterial community diversity in both ASD and healthy children, while a greater increase was found in ASD children than that of healthy children. Furthermore, this study successfully isolated and identified some representative nanoparticle-resistant bacteria based on the color, shape, and edge of colony as well as the 16S rDNA sequence analysis. The community of nanoparticle-resistant bacteria differed in between healthy and ASD children. Indeed, the representative strains 6-1, 6-2, 6-3 and 6-4 from healthy children were identified as Bacillus anthracis, Escherichia coli, Bacillus cereus and Escherichia coli with sequence similarity of 97.86%, 99.86%, 99.03% and 99.65%, respectively, while the representative strains 8-1, 8-2 and 8-3 from ASD children were identified as Bacillus cereus, with sequence similarities of 99.58%, 99.72% and 99.72%, respectively. Overall, this study demonstrated that ZnONPs caused a change in number, diversity, and species composition of gut bacteria, but differed in healthy and ASD children.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Impact of Zinc Oxide Nanoparticles on the Composition of Gut Microbiota in Healthy and Autism Spectrum Disorder Children

Jiang

Zhou

et al. 2021

Materials

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“…Positive correlation of abundance of Phylum Firmicutes with five behavioral domains: gross motor skills, fine motor, adaptation, personal-social, and language, have been reported with significant Pearson's r = 0.327 and P-value < 0.05 in autism. ( Ding et al., 2020 ; Zhang et al., 2021 ). A higher abundance of Firmicutes indicates that they have not impacted the autism behavior at the phylum level.…”

Section: Discussionmentioning

confidence: 99%

Mucosa-associated specific bacterial species disrupt the intestinal epithelial barrier in the autism phenome

Agarwala

Ramachandra

2021

Brain, Behavior, & Immunity - Health

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Gut-Brain Axis provides a bidirectional communicational route, an imbalance of which can have pathophysiological consequences. Differential gut microbiome studies have become a frontier in autism research, affecting 85% of autistic children. The present study aims to understand how gut microbiota of autism subjects differ from their neurotypical counterparts. This study would help to identify the abundance of bacterial signature species in autism and their associated metabolites. 16S rRNA metagenomic sequence datasets of 30 out of 206 autism subjects were selected from the American Gut Project Archive. First, the taxonomic assignment was inferred by similarity-based methods using the Quantitative Insights into Microbial Ecology (QIIME) toolkit. Next, species abundance was characterized, and a co-occurrence network was built to infer species interaction using measures of diversity. Thirdly, statistical parameters were incorporated to validate the findings. Finally, the identification of metabolites associated with these bacterial signature species connects with biological processes in the host through pathway analysis. Gut microbiome data revealed Akkermansia sp. and Faecalibacterium prausnitzii to be statistically lower in abundance in autistic children than their neurotypical peers with a five and two-fold decrease, respectively. While Prevotella sp. and Sutterella sp. showed a five and a two-fold increase in cases, respectively. The constructed pathway revealed succinate and butyrate as the significant metabolites for the bacterial signature species identified. The present study throws light on the role of mucosa-associated bacterial species: Veillonella sp. , Prevotella sp. , Akkermansia sp. , Sutterella sp. , Faecalibacterium prausnitzii, Lactobacillus sp., which can act as diagnostic criteria for detection of gut dysbiosis in autism.

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“…Alguns autores relataram diminuição na abundância relativa (Liu et al, 2019;Ma et al, 2019) e alterações na composição da família Lachnospiraceae (De Angelis et al, 2013), enquanto outros encontraram aumento da abundância relativa desta mesma família de bactérias (Ding et al, 2020).…”

Section: Desenvolvimentounclassified

Alterações Na Microbiota Gastrointestinal De Crianças Com Transtorno Do Espectro Autista: Uma Revisão Sistemática

2021

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Researchs about the causes of Autistic Spectrum Disorder (ASD) has led several scientists to relate many others systems to the patient's brain, linking changes in the gastrointestinal microbiota (GIM) to typical behaviors of people with ASD, suggesting that the brain-intestine axis is an important piece in this 'puzzle'. This work aimed to investigate the microbiota gastrointestinal in children with ASD. Such research was carried out on scientific work platforms, resulting in 293 works, which, after the selection, totaled 28 works analyzed. Almost all of them showed changes in the intestinal microbiota of children with ASD, the reduction of their diversity being the main alteration of these individuals in relation to the control group. The bacterial family Lachnospiraceae, the bacterial gender Lactobacillus, Clostridium, Feacalibacterium, and Bacteroides stood out in these studies; and the fungal genus Candida. Among the symptoms, constipation was quite frequent in children with ASD and some groups of microorganisms were positively or negatively associated with changes in sleep, speech development and behavioral aspects. We conclude that there are several alterations of GIM in children with autism when compared to children with typical neurodevelopment, being also affected by anatomical and metabolic issues as causes or consequences of ASD. There is a need for further studies, especially in the Brazilian population, and more specifically in the states, in view of the great heterogeneity of our people and their culture, the great dietary variations of the last decades, as well as the increase in gene flow among world populations.

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Gut microbiota changes in patients with autism spectrum disorders

Cited by 105 publications

References 58 publications

Impact of Zinc Oxide Nanoparticles on the Composition of Gut Microbiota in Healthy and Autism Spectrum Disorder Children

Impact of Zinc Oxide Nanoparticles on the Composition of Gut Microbiota in Healthy and Autism Spectrum Disorder Children

Mucosa-associated specific bacterial species disrupt the intestinal epithelial barrier in the autism phenome

Alterações Na Microbiota Gastrointestinal De Crianças Com Transtorno Do Espectro Autista: Uma Revisão Sistemática

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