Gut microbiota and holobiont metabolome composition of the medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa

Foucault, Pierre; Gallet, Alison; Duval, Clément; Marie, Benjamin; Duperron, Sébastien

doi:10.1016/j.aquatox.2022.106329

Cited by 11 publications

(16 citation statements)

References 36 publications

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“…Besides these two dominant genera, a diversity of not necessarily abundant ASVs is also observed only at these later stages, suggesting a community enriched in additional gut specialists. Overall, medaka sampled at d120 display a gut-associated community highly similar to that previously documented in older adult specimens reared in the same facility, suggesting that the community converges towards a certain level of stability by then [18]. Interestingly, community shifts observed herein are very comparable in terms of taxa involved and succession to those documented in the zebrafish [21,33], and also display some congruency with other investigated non-model fish species that could suggest general trends in teleost fish.…”

Section: Community Shifts Occur Throughout Developmentsupporting

confidence: 72%

“…is the fastest responding compartment, with significant changes in taxa relative abundances occurring within the first 24 hours, emphasizing its potential importance for the holobionts response [18]. In these three studies, a limited number of intestinal bacterial taxa were shown to be major actors of the symbiotic system and its response, and responded in a quite reproducible and predictable way.…”

Section: Introductionmentioning

confidence: 92%

“…Major changes in community composition were also documented between 35 and 75 dpf in the zebrafish, with dominance of Fusobacteria and Firmicutes from 75 dpf onwards [21]. Cetobacterium and ZOR0006 are two of the most dominant gut-associated bacteria identified in all our previous studies on the medaka from the MNHN facility [16][17][18]. Cetobacterium is a major non-pathogenic gut resident bacterium in many fish species including zebrafish and tilapia [33,[41][42][43].…”

Section: Community Shifts Occur Throughout Developmentmentioning

confidence: 97%

“…Similarly, ZOR0006 is a gut specialist bacterium, of which the abundances were shown to drop dramatically upon exposure to the same cyanobacterial metabolites, and with abilities to degrade various carbohydrates [17]. A drop in the relative abundance of either of these two dominant bacteria is considered a sign of dysbiosis in medaka fish [12,18]. Interestingly, an unclassified Firmicutes (CK-1C4-19) was also shown to become abundant in adult zebrafish while unabundant in earlier stages, suggesting similarities between the two model species [21].…”

Section: Community Shifts Occur Throughout Developmentmentioning

confidence: 99%

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Establishment of the Bacterial Microbiota in A Lab-Reared Model Teleost Fish, the Medaka <em>Oryzias Latipes</em>

Duval¹,

Marie²,

Foucault³

et al. 2022

Preprint

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Oryzias latipes is an important model organism for physiology, genetics and developmental studies, and has also emerged a relevant vertebrate model for aquatic ecotoxicology. Knowledge regarding its associ-ated microbiota on the other hand is still scarce and limited to adults, despite the relevance of associated microbiome to hosts biology. This study provides first insights into the establishment of bacterial mi-crobiota during early developmental stages of laboratory-reared medaka using a 16SrRNA sequenc-ing-based approach. Major shifts in community compositions are observed, from a Proteobacte-ria-dominated community in larvae and juveniles to a more phylum diverse community towards adult-hood, with no obvious difference between female and male specimens. Major bacterial taxa found in adults, including genera Cetobacterium and ZOR0006, establish progressively and are rare during early stages. Dominance shifts are comparable to those documented in another major model teleost, the zebrafish. Results from this study provide a basis for future work investigating the influence of meda-ka-associated bacteria during host development.

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Section: Community Shifts Occur Throughout Developmentsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 92%

Section: Community Shifts Occur Throughout Developmentmentioning

confidence: 97%

Section: Community Shifts Occur Throughout Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Establishment of the Bacterial Microbiota in A Lab-Reared Model Teleost Fish, the Medaka <em>Oryzias Latipes</em>

Duval¹,

Marie²,

Foucault³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The dynamics of biofilm meta-metabolome changes versus compositions of prokaryotic and eukaryotic biofilm communities were investigated by comparing trajectories of centroids using the Multivariate Omics Trajectory Analysis method (MOTA) (Foucault et al, 2022). Datasets of meta-metabolome, prokaryotic and eukaryotic biofilm communities were log 2 transformed and analyzed identically.…”

Section: Methodsmentioning

confidence: 99%

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

Colas,

Marie,

Morin

et al. 2023

Preprint

Self Cite

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Untargeted metabolomics is a non-a priorianalysis of biomolecules that characterizes the metabolome variations induced by short- and long-term exposures to stressors. Even if the metabolite annotation remains lacunar due to database gaps, the global metabolomic fingerprint allows for trend analyses of dose-response curves for hundreds of cellular metabolites. The combination of untargeted metabolomic features and benchmark-dose (BMD) calculations then makes it possible to determine concentration range inducing defense responses (CRIDeR) and concentration range inducing damage responses (CRIDaR). To develop this approach in a context of time-dependent microbial community changes, mature river biofilms were exposed for 1 month to four cobalt (Co) concentrations (background concentration, 1 x 10-7, 5 x 10-7and 1 x 10-6M) in an open system of artificial streams. The meta-metabolomic response of biofilms was compared against a multitude of biological parameters (including bioaccumulation, biomass, chlorophyllacontent, composition and structure of prokaryotic and eukaryotic communities) monitored at set exposure times (from 1 hour to 28 days). Cobalt exposure induced extremely rapid responses of the meta-metabolome, with time range inducing defense responses (TRIDeR) of around ten seconds, and time range inducing damage responses (TRIDaR) of several hours. Even in biofilms whose structure had been altered by Co bioaccumulation (reduced biomass, chlorophyllacontents and changes in the composition and diversity of prokaryotic and eukaryotic communities), CRIDeRs with similar initiation thresholds (1.41 ± 0.77 x 10-10M Co2+added in the exposure medium) were set up at the meta-metabolome level at every time point. In contrast, the CRIDaR initiation thresholds increased by 10 times in long-term Co exposed biofilms. The present study demonstrates that defense and damage responses of biofilm meta-metabolome exposed to Co are rapidly and sustainably impacted, even within tolerant and resistant microbial communities.Graphical abstractHighlightsProkaryotic community structures were impacted after 1 h of exposure to CoBiofilm meta-metabolome was impacted after 36 s of exposure to CoBiofilm meta-metabolome response was faster than changes in biofilm communitiesShort- and long-term exposed biofilms have similar CRIDeR initiation thresholdsLong-term exposed biofilms have higher CRIDaR initiation thresholds

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Flunitrazepam and its metabolites exposure disturb the zebrafish gut-liver axis: Combined microbiome and metabolomic analysis

Lin,

Qin,

Wang

et al. 2023

Aquatic Toxicology

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Gut microbiota and holobiont metabolome composition of the medaka fish (Oryzias latipes) are affected by a short exposure to the cyanobacterium Microcystis aeruginosa

Cited by 11 publications

References 36 publications

Establishment of the Bacterial Microbiota in A Lab-Reared Model Teleost Fish, the Medaka <em>Oryzias Latipes</em>

Establishment of the Bacterial Microbiota in A Lab-Reared Model Teleost Fish, the Medaka <em>Oryzias Latipes</em>

New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities

Flunitrazepam and its metabolites exposure disturb the zebrafish gut-liver axis: Combined microbiome and metabolomic analysis

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