2009
DOI: 10.1038/cdd.2009.44
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Abstract: Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, … Show more

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Cited by 588 publications
(490 citation statements)
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“…For cytofluorometric analyses, isotype-matched IgG antibodies (Cell Signaling Technology, Danvers, MA, USA) were used as control conditions, and statistical analyses -limited to live (PI À ) cells -were performed by means of the Cell Quest Software package. 58,59 Cell death assays. Apoptotic cell death was quantified based on the exposure of phosphatidylserine on the cell surface and plasma membrane permeabilization.…”
Section: Methodsmentioning
confidence: 99%
“…For cytofluorometric analyses, isotype-matched IgG antibodies (Cell Signaling Technology, Danvers, MA, USA) were used as control conditions, and statistical analyses -limited to live (PI À ) cells -were performed by means of the Cell Quest Software package. 58,59 Cell death assays. Apoptotic cell death was quantified based on the exposure of phosphatidylserine on the cell surface and plasma membrane permeabilization.…”
Section: Methodsmentioning
confidence: 99%
“…Weakly invasive luminal-like (MCF-7 and ZR-75-1) and highly invasive basal-like (MDA-MB-231 and Hs578T) human breast cancer cell lines were embedded for up to 8 days in 3D COL1 gels and examined for morphological characteristics of apoptotic cells, including membrane blebbing, nuclear condensation and fragmentation (Galluzzi et al, 2009). MDA-MB-231 and Hs578T cells displayed an elongated fibroblast-like morphology characterized by the presence of invasive cytoplasmic extensions ( Figure 1a).…”
Section: D Col1 Induces Apoptosis In Poorly Invasive Breast Cancer Cmentioning
confidence: 99%
“…For the simultaneous quantification of plasma membrane integrity and mitochondrial transmembrane potential (Δψ m ), cells were collected and stained with 1 μg/ mL propidium iodide (PI), which only incorporates into dead cells, and 40 nM 3,3'-dihexyloxacarbocyanine iodide [DiOC 6 (3)], a Δψ m -sensitive dye (Molecular Probes-Invitrogen), for 30 min at 37°C. 51,52 For the assessment of cell cycle distribution, cells were collected, washed once with 0.1% (w/v) D-glucose (Sigma-Aldrich) in PBS and then fixed by gentle vortexing in ice-cold 80% (v/v) ethanol (Carlo Erba Reagents) for 30 sec. After overnight incubation at -20°C, samples were centrifuged to remove ethanol and stained with 50 μg/mL PI in 0.1% (w/v) D-glucose in PBS supplemented with 1 μg/mL (w/v) RNase A (Sigma-Aldrich) for 30 min at 37°C.…”
Section: Disclosure Of Potential Conflicts Of Interestmentioning
confidence: 99%