Guanylin strongly stimulates rat duodenal HCO3- secretion: Proposed mechanism and comparison with other secretagogues

Guba, M.; Kühn, Martin; Wg, Forssmann; Claßen, Martin; Gregor, Michael; Seidler, Ursula

doi:10.1016/s0016-5085(96)70018-5

Cited by 112 publications

(62 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the results obtained in Ussing chamber studies of isolated adult tissues, a deficit in STa-stimulated secretion was not observed in the intestines of 4 -5-day-old NKCC1-deficient mice. In adult tissues, anion secretion in response to STa or to guanylin or uroguanylin, the endogenous ligands for the receptor that is activated by STa, has been shown to be dependent on CFTR activity (33)(34)(35)(36)(37) and to be inhibited by bumetanide (35). It is possible that the differences observed between the Ussing chamber and suckling mouse assays are related to the age of the animals, with NKCC1 contributing little to electrolyte uptake needed for stimulated secretion in the 4 -5-day-old mouse but contributing significantly to this process in the adult.…”

Section: Nkcc1mentioning

confidence: 99%

Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly Deaf

Flagella¹,

Clarke²,

Miller³

et al. 1999

Journal of Biological Chemistry

358

349

View full text Add to dashboard Cite

In chloride-secretory epithelia, the basolateral Na-K2Cl cotransporter (NKCC1) is thought to play a major role in transepithelial Cl ؊ and fluid transport. Similarly, in marginal cells of the inner ear, NKCC1 has been proposed as a component of the entry pathway for K ؉ that is secreted into the endolymph, thus playing a critical role in hearing. To test these hypotheses, we generated and analyzed an NKCC1-deficient mouse. Homozygous mutant (Nkcc1 ؊/؊ ) mice exhibited growth retardation, a 28% incidence of death around the time of weaning, and mild difficulties in maintaining their balance. Mean arterial blood pressure was significantly reduced in both heterozygous and homozygous mutants, indicating an important function for NKCC1 in the maintenance of blood pressure. cAMP-induced short circuit currents, which are dependent on the CFTR Cl ؊ channel, were reduced in jejunum, cecum, and trachea of Nkcc1 ؊/؊ mice, indicating that NKCC1 contributes to cAMP-induced Cl ؊ secretion. In contrast, secretion of gastric acid in adult Nkcc1 ؊/؊ stomachs and enterotoxin-stimulated fluid secretion in the intestine of suckling Nkcc1 ؊/؊ mice were normal. Finally, homozygous mutants were deaf, and histological analysis of the inner ear revealed a collapse of the membranous labyrinth, consistent with a critical role for NKCC1 in transepithelial K ؉ movements involved in generation of the K ؉ -rich endolymph and the endocochlear potential.

show abstract

Section: Nkcc1mentioning

confidence: 99%

Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly Deaf

Flagella¹,

Clarke²,

Miller³

et al. 1999

Journal of Biological Chemistry

358

349

View full text Add to dashboard Cite

show abstract

“…This channel, present in pancreatic ducts, duodenal epithelial cells, and Brunner's glands (44), is closely associated with epithelial HCO 3 -secretion. NPPB, a blocker of the CFTR Cl -channel, inhibits bicarbonate secretion stimulated by cGMP agonists in rats (45) and by cAMP in mice (42), suggesting that bicarbonate exits from the cells through the CFTR itself or through CFTR-associated transporters. The mechanism by which bicarbonate is secreted across epithelia is controversial.…”

Section: Figurementioning

confidence: 99%

Cellular bicarbonate protects rat duodenal mucosa from acid-induced injury

Akiba

Furukawa²,

Guth³

et al. 2001

J. Clin. Invest.

View full text Add to dashboard Cite

“…cGMP can either stimulate cGMP-dependent protein kinase (PKG) or lead to increases in cyclic adenosine monophosphate (cAMP) levels that stimulate cAMP-dependent protein kinase (PKA); both PKG and PKA activate CFTR (3,4,9,13,34,42). In mammals, this leads to Cl Ϫ , fluid, and HCO 3 Ϫ secretion into the intestinal lumen to help neutralize the effects of acidic chyme (2,5,30,54). This process is especially important after the ingestion of food, where GN and UGN are secreted into the intestine, bind GC-C, and elevate the levels of intracellular cGMP in enterocytes (3).…”

mentioning

confidence: 99%

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Ruhr

Bodinier

Mager

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

The physiological effects of guanylin (GN) and uroguanylin (UGN) on fluid and electrolyte transport in the teleost fish intestine have yet to be thoroughly investigated. In the present study, the effects of GN, UGN, and renoguanylin (RGN; a GN and UGN homolog) on short-circuit current ( Isc) and the transport of Cl−, Na+, bicarbonate (HCO3−), and fluid in the Gulf toadfish ( Opsanus beta) intestine were determined using Ussing chambers, pH-stat titration, and intestinal sac experiments. GN, UGN, and RGN reversed the Isc of the posterior intestine (absorptive-to-secretory), but not of the anterior intestine. RGN decreased baseline HCO3− secretion, but increased Cl− and fluid secretion in the posterior intestine. The secretory response of the posterior intestine coincides with the presence of basolateral NKCC1 and apical cystic fibrosis transmembrane conductance regulator (CFTR), the latter of which is lacking in the anterior intestine and is not permeable to HCO3− in the posterior intestine. However, the response to RGN by the posterior intestine is counterintuitive given the known role of the marine teleost intestine as a salt- and water-absorbing organ. These data demonstrate that marine teleosts possess a tissue-specific secretory response, apparently associated with seawater adaptation, the exact role of which remains to be determined.

show abstract

Guanylin strongly stimulates rat duodenal HCO3- secretion: Proposed mechanism and comparison with other secretagogues

Cited by 112 publications

References 4 publications

Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly Deaf

Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly Deaf

Cellular bicarbonate protects rat duodenal mucosa from acid-induced injury

Guanylin peptides regulate electrolyte and fluid transport in the Gulf toadfish (Opsanus beta) posterior intestine

Contact Info

Product

Resources

About