Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation

Abstract

Background: Gualou Xiebai Decoction (GLXB) is a classic prescription of Chinese medicine used for the treatment of cardiac problems. The present study was designed to explore the effect and mechanism of GLXB on ischemia/reperfusion (I/R) induced disorders in myocardial structure and function, focusing on the regulation of energy metabolism and the RhoA/ROCK pathway.Methods: After hyperlipidemic rat model was established by oral administration of high fat diet, the rats were treated with GLXB for 6 weeks and subjected to 30 min occlusion of the left anterior descending coronary artery (LADCA) followed by 90 min reperfusion to elicit I/R challenge. Myocardial infarct size was assessed by Evans blue-TTC staining. Myocardial blood flow (MBF) and cardiac function were evaluated. Enzyme-linked immunosorbent assay was performed to examine the content of ATP, ADP, AMP, CK, CK-MB, LDH, cTnT, cTnI, and IL-6. Double staining of F-actin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. Expressions of ATP synthase subunit δ (ATP 5D), and RhoA and ROCK were determined by Western blotting.Results: Administration with GLXB at high dose for 6 weeks protected heart against I/R-induced MBF decrease, myocardial infarction and apoptosis, ameliorated I/R-caused impairment of cardiac function and myocardial structure, restored the decrease in the ratio of ADP/ATP and AMP/ATP, and the expression of ATP 5D with inhibiting the expression of RhoA and ROCK.Conclusions: Treatment with GLXB effectively protects myocardial structure and function from I/R challenge, possibly via regulating energy metabolism involving inactivation of RhoA/ROCK signaling pathway.

Gualou Xiebai Banxia decoction ameliorates Poloxamer 407-induced hyperlipidemia

“…As shown in the present study, the clinical condition of rats can be improved by GLXBBX through a significant reduction in plasma TG levels, though with no resulting change in plasma TC. Gualou Xiebai decoction is a classic prescription in Chinese medicine used for the treatment of cardiac conditions, but also serves many other functions, including antioxidation, anti-apoptosis, and anti-inflammation [ 28 ]. Decoctions containing Banxia have also been used for hyperlipidemia treatment.…”

Traditional Chinese medicine combined with pulmonary drug delivery system and idiopathic pulmonary fibrosis: Rationale and therapeutic potential

“…Danlou Prescription (DLP) is developed from the TCM formula Gualou Xiebai Decoction [ 48 ]. It is composed of ten herbs: Trichosanthes kirilowii Maxim (Gualoupi), Allium macrostemon Bunge, Puerariae Lobatae Radix, Salvia miltiorrhiza Bunge, Astragalus mongholicus Bunge, Davallia trichomanoides Blume, Paeonia lactiflora Pall, Alisma plantago-aquatica L., Ligusticum chuanxiong Hort and Curcuma aromatica Salisb.…”

Modulatory Effects of Chinese Herbal Medicines on Energy Metabolism in Ischemic Heart Diseases

“…GLXB is composed of Trichosanthis Pericarpium, Allium macrostemon Bunge and wine, has been proposed in AD 200-205 by famous doctor Zhang Zhong-Jing. Rats treated with GLXB exhibited a significant reduction in myocardial infarct size, as well as improved cardiac function and myocardial structure following myocardial I/R injury, which was likely achieved through the modulation of energy metabolism via the inhibition of the RhoA/ ROCK signaling pathway (Yan et al, 2018).…”