K. H. Steinkraus, R. P. Mortlock, R. R. Granados, K. A. McKenna, M. G. Villani, P. S. Robbins
TNM-FH Lepiabpreran insect cell culture medium containing 10% fetal bovine serum (FBS), while allowing limited vegetative growth of Paenibacillus larvae (wild-type strain), the causative agent of American foulbrood, contained no viable vegetative cells upon subculture, nor were any heat resistant spores produced in this medium alone. However, TNM-FH medium containing embryonic or midgut cells from Trichoplusia ni, hemocytes from Estigmene acreu. ovarian and embryonic cells from Spoahpteru frugiperda, embryoni…
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