Growth of Pseudomonas putida at low temperature: global transcriptomic and proteomic analyses

Abstract

In its natural habitats (soil, water and rhizosphere), Pseudomonas putida can suffer frequent and long-term changes in temperature that affect its growth and survival. Pseudomonas putida KT2440, a well-characterized model strain, grows optimally at 30°C but can proliferate at temperatures as low as 4°C. However, little information is available on the physiological changes that occur when P. putida grows at low temperatures. To investigate this area, the transcriptome and proteome profiles of cells exponentially growing in a complex medium at 10°C were compared with those of cells exponentially growing at 30°C. Low temperature modified the expression of at least 266 genes (some 5% of the genome). Many of the genes showing differential expression were involved in energy metabolism or in the transport and binding of substrates, although genes implicated in other cellular functions were also affected. Several changes seemed directed towards neutralizing problems created by low temperature, such as increased protein misfolding, the increased stability of DNA/RNA secondary structures, reduced membrane fluidity and a reduced growth rate. The present results improve our understanding of the P. putida lifestyle at low temperature, which may be relevant for its applications in bioremediation and in promotion of plant growth.

Influence of temperature on the growth of Pseudomonas putida

“…Hence, the optimum temperature of our test bacterial strain of Pseudomonas putida (SUBP03) was recorded to be 30 °C. Present finding is quite consistent with the earlier studies where P. putida has been noticed to exhibit highest growth at 30 °C too (3)(4)(5).…”

“…. Notably, bacteria were found to utilize unified strategies to deal with the environmental stress (3)(4)(5)(24)(25)(26). Nevertheless, several studies apparently suggested the expression of the global molecular chaperones, which maintain the cellular homeostasis, including rpoE, rpoS and rpoH genes in E. coli; dnaK, dnaJ, and grpE in Pseudomonas spp.…”

Response of Vibrio cholerae to Low-Temperature Shifts: CspV Regulation of Type VI Secretion, Biofilm Formation, and Association with Zooplankton

“…Many of these changes in gene expression are presumably oriented toward overcoming the challenges imposed by cold shock. Studies aimed at characterizing the cold shock responses of other organisms have commonly identified a number of genes involved in DNA modulation, transport and binding functions, membrane fluidity, cell envelope modification, and energy metabolism (16,(58)(59)(60)(61)(62)(63). We found similar genes that exhibited differential expression upon a low-temperature shift in V. cholerae as well (Table S1).…”

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

“…These processes were done aseptically inside a biosafety cabinet, as well in according to standard. It was indicated that multiple loops seeding ensure proper bacterial growth [3]. The orbital shaker was operated at 37℃ and 180 rpm.…”

“…Upon inoculation into a suitable media, this gram negative, aerobic and gammaproteo bacterium selectively takes up nutrients from the medium for biosynthesis [3]. The initial lag growth phase is a period of cell change in accordance with the new environment.…”