Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei

Hou, Cuihong; Song, Shougang; Zhu, Lulu; Shi, Lili; Tan, Beiping; Zhang, Shuang

doi:10.3389/fmars.2021.685429

Cited by 4 publications

(5 citation statements)

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“…Transcriptome sequencing, a common tool in biology research at present, is also extensively used to analyses the mechanisms associated with protein source substitution in diets on the growth, metabolism and immunity of organisms [ 21 , 34 , 35 ]. In the present study, a comparative transcriptome analysis of L. vannamei containing the CPC and FM groups was performed to investigate the mechanism of the effect of the CPC substitution for fishmeal.…”

Section: Discussionmentioning

confidence: 99%

“…Hemolymph from another three shrimp randomly selected from each tank was withdrawn into modified ACD anticoagulant solution and centrifuged for 5 min (3000× g at 4 °C) to isolate hemocytes, using as a sample. The hemocytes were stored at −80 °C for RNA extraction and used for the detection of gene expression and transcriptomic analysis, as our previous study showed [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…Before the filtered reads were mapped to the L. vannamei reference genome (NCBI Genome database ID: No. PRJNA438566), using HISAT software [ 21 ], raw reads generated by Illumina Hiseq 2000 from the twelve libraries were cleaned using SeqPrep ( ; accessed on 1 October 2021) and Sickle ( ; accessed on 1 October 2021) software by removing reads with adaptors, reads with more than 10% Q < 20 bases (those with a base quality less than 20), and low-quality sequences (reads with ambiguous bases ‘N’).2.4.3. Serum Biochemical Parameters…”

Section: Methodsmentioning

confidence: 99%

“…High-throughput sequencing has become a routine experimental technique used in the field of life science [ 21 ]. Transcriptome sequencing (RNA-SEQ) is a new technology that uses high-throughput sequencing to study gene transcription, which can comprehensively and quickly obtain the sequence and expression information of almost all transcripts of a specific cell or tissue in a certain state [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…High-throughput sequencing has also been widely used in transcriptome analysis to study the mechanisms of growth, development, immunity, and other related life activities. Hou et al used transcriptome sequencing analysis to find that dietary TWS119 may improve growth performance and immune resistance in L. vannamei by activating the Wnt/β-catenin pathway and inhibiting the activity of LvGSK3β [ 21 ]. Yin et al employed transcriptome sequencing analysis to discover that lipids from L. vannamei are a source of nutrition for Vibrio vulnificus , and they are identified by regulating lipid homeostasis to avoid being infected [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Reveals the Immunoregulation of Replacing Fishmeal with Cottonseed Protein Concentrates on Litopenaeus vannamei

Wang

Chen

et al. 2023

Animals

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Cottonseed protein concentrate (CPC) is a new non-food protein source with high crude protein, low price, and abundant resources, making it an ideal substitute for fishmeal. In this study, we investigated the effects of CPC re placing fishmeal on the immune response of Litopenaeus vannamei using transcriptome sequencing. L. vannamei (initial body weight: 0.42 ± 0.01 g) were fed four isonitrogenous and isolipid feeds for eight weeks, with CPC replacing fishmeal at 0% (control, FM), 15% (CPC15), 30% (CPC30), and 45% (CPC45), respectively. At the end of the feeding trial, the changes of the activities and expression of immune-related enzymes were consistent in L. vannamei in the CPC-containing group when compared with the FM group. Among them, the activities of ACP, PO, and LZM in the group whose diet was CPC30 were significantly higher than those in the FM group. Moreover, the activities of AKP, SOD, and CAT were significantly higher in the group containing CPC than in the FM group. Furthermore, all CPC groups had considerably lower MDA levels than the FM group. This suggests that the substitution of fishmeal with CPC leads to a significant immune response in L. vannamei. Compared with the FM group, transcriptome analysis identified 805 differentially expressed genes (DEGs) (484 down and 321 up), 694 (266 down and 383 up), and 902 (434 down and 468 up) in CPC15, CPC30, and CPC45, respectively. Among all DEGs, 121 DEGs were shared among different CPC-containing groups compared with the FM group. Most of these differential genes are involved in immune-related signaling pathways. The top 20 signaling pathways enriched for differential genes contained toxoplasmosis, pathogenic Escherichia coli infection, insulin resistance, and Toll and immune deficiency (IMD) pathways, in which NF-kappa-B inhibitor Cactus were involved. In addition, trend analysis comparison of the DEGs shared by the group with CPC in the diet and the FM group showed that Cactus genes were significantly down-regulated in the group with CPC in the diet and were lowest in the CPC30 group. Consistently, the expression of antimicrobial peptide genes was significantly higher in both diet-containing CPC groups than in the FM group. In conclusion, the moderate amount of CPC substituted for fishmeal may improve the immunity of L. vannamei by suppressing the expression of Cactus genes, thereby increasing the expression of antimicrobial peptide (AMP) genes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%