Growth hormone (GH) treatment reduces peripheral thyroid hormone action in girls with Turner syndrome

Susperreguy, Sebastián; Miras, Mirta; Montesinos, María M.; Mascanfroni, Iván; Muñoz, Liliana; Sobrero, Gabriela; Silvano, Liliana; Masini-Repiso, Ana M.; Coleoni, Aldo H.; Targovnik, Héctor M.; Pellizas, Claudia G.

doi:10.1111/j.1365-2265.2007.02936.x

Cited by 10 publications

(16 citation statements)

References 40 publications

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“…Previously, we reported data showing that IGF‐I and GH reduced specific T3 responses in rat tissues by a down‐regulation of TR in both rat cell culture and in vivo. 9–11 In consonance, we provided evidence for a reduced peripheral TH action induced by GH treatment to Turner Syndrome (TS) girls assessed by the measurement of TR mRNA expression in peripheral blood mononuclear cells (PBMC) and serum biochemical markers of TH action at tissue level 12 . However, these results could not be extrapolated to other patients receiving GH treatment, taking into consideration the different endogenous GH secretion as well as the different GH dosage administered to patients carrying different etiopathogenic growth deficiencies 13 .…”

Section: Introductionmentioning

confidence: 81%

Growth hormone treatment in children with idiopathic short stature: correlation of growth response with peripheral thyroid hormone action

Susperreguy¹,

Muñoz²,

Tkalenko³

et al. 2011

Clinical Endocrinology

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Section: Introductionmentioning

confidence: 81%

Growth hormone treatment in children with idiopathic short stature: correlation of growth response with peripheral thyroid hormone action

Susperreguy¹,

Muñoz²,

Tkalenko³

et al. 2011

Clinical Endocrinology

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“…Primers were designed to distinguish cDNA and genomic DNA/pseudogenes (Kreuzer et al 1999). Primers were from Sigma (Sigma-Aldrich from Buenos Aires, Argentina) and designed to amplify bands of 591, 650 and 273 bp for TR 1 , 1 mRNA and -actin mRNAs, respectively; according to the following sequence: TR 1 forward: 5Ј-TTCAGCGAGTTTACCAAG ATCATCAC-3Ј, TR 1 reverse: 5Ј-TTAGACTTCCTGAT CCTCAAAGACCTC-3Ј; TR 1 forward: 5Ј-GTGACCGT GTAGAGTAGATG-3Ј, TR 1 reverse: 5Ј-CTCCACACCA AGTCTACAGC-3Јand -actin forward: 5Ј-CGGAACCG CTCATTGCC-3Ј, -actin reverse: 5Ј-ACCCACACTGTG CCCATCTA-3Ј (Montesinos et al 2006;Susperreguy et al 2007). PCR was carried out in a 20 l Wnal vol: 1.5 mM MgCl 2 , 4 l 5£ PCR buVer, 1 U Taq-polymerase (Promega, Madison, WI, USA), 0.25 mM each dNTP (Promega, Madison, WI, USA) and 2 l RT product.…”

Section: Total Rna Extractionmentioning

confidence: 99%

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

Paul

Mukdsi

Pellizas

et al. 2008

Histochem Cell Biol

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The objectives of the present work were to assess whether epithelial cells from the different segments of epididymis express TR alpha 1-beta 1 isoforms, to depict its subcellular immunolocalization and to evaluate changes in their expression in rats experimentally submitted to a hypothyroid state by injection of 131I. In euthyroid and hypothyroid groups, TR protein was expressed in epididymal epithelial cells, mainly in the cytoplasmic compartment while only a few one showed a staining in the nucleus as well. A similar TR immunostaining pattern was detected in the different segments of the epididymis. In hypothyroid rats, the number of TR-immunoreactive epithelial cells as well as the intensity of the cytoplasmic staining significantly increased in all sections analyzed. In consonance to the immunocytochemical analysis, the expression of TR alpha 1-beta 1 isoforms, assessed by Western blot revealed significantly higher levels of TR in cytosol compared to the nuclear fractions. Furthermore, TR expression of both alpha 1 and beta 1 isoforms and their mRNA levels were increased by the hypothyroid state. The immuno-electron-microscopy showed specific reaction for TR in principal cells associated with eucromatin, cytosolic matrix and mitochondria. The differences in expression levels assessed in control and thyroidectomized rats ascertain a specific function of TH on this organ.

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“…Of four studies identified using variable pharmacological doses of GH therapy in girls with Turner’s syndrome, two demonstrated no change in thyroid parameters 62,63 one showed a decrease in free T4 within the normal range at 6 months of therapy that returned to baseline at 1 year without any alteration in T3 64 and the most recent study found a trend towards a decrease in free T4 and an increase in T3 and TSH, all within the normal range 65 . None of the subjects became clinically or biochemically hypothyroid during GH administration, suggesting that GH unmasks rather than induces CH in at risk groups with organic pituitary disease.…”

Section: Growth Hormone Effect On the Thyroid Axismentioning

confidence: 99%

The interaction between growth hormone and the thyroid axis in hypopituitary patients

Behan

Monson

Agha

2011

Clinical Endocrinology

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SummaryAlterations in the hypothalamo-pituitary-thyroid axis have been reported following growth hormone (GH) administration in both adults and children with and without growth hormone deficiency. Reductions in serum free thyroxine (T4), increased tri-iodothyronine (T3) with or without a reduction in serum thyroid-stimulating hormone secretion have been reported following GH replacement, but there are wide inconsistencies in the literature about these perturbations. The clinical significance of these changes in thyroid function remains uncertain. Some authors report the changes are transient and revert to normal after a few months or longer. However, in adult hypopituitary patients, GH replacement has been reported to unmask central hypothyroidism biochemically in 36-47% of apparently euthyroid patients, necessitating thyroxine replacement and resulting in an attenuation of the benefit of GH replacement on quality of life in those who became biochemically hypothyroid after GH replacement. The group at highest risk are those with organic pituitary disease or multiple pituitary hormone deficiencies. It is therefore prudent to monitor thyroid function in hypopituitary patients starting GH therapy to identify those who will develop clinical and biochemical features of central hypothyroidism, thus facilitating optimal and timely replacement.

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Growth hormone (GH) treatment reduces peripheral thyroid hormone action in girls with Turner syndrome

Abstract: GH treatment reduced TR expression in PBMC and biochemical serum markers of TH action. These results suggest that GH treatment in TS patients impair peripheral TH action at tissue level and prompt a role in the reduced growth response to the therapy.

Cited by 10 publications

References 40 publications

Growth hormone treatment in children with idiopathic short stature: correlation of growth response with peripheral thyroid hormone action

Growth hormone treatment in children with idiopathic short stature: correlation of growth response with peripheral thyroid hormone action

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

The interaction between growth hormone and the thyroid axis in hypopituitary patients

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