Growth dynamics and differential accumulation of picrosides and its precursor metabolites in callus cell lines of Picrorhiza kurroa with distinct anti-steatotic potential

Partap, Mahinder; Chhimwal, Jyoti; Kumar, Pawan; Kumar, Dinesh; Padwad, Yogendra; Warghat, Ashish R.

doi:10.1016/j.procbio.2022.06.002

Cited by 5 publications

(1 citation statement)

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“…It is characterized by the following advantages: growth in controlled conditions; absence of restrictions in the action of seasonal, climatic and geographical factors; preservation of the ability to form metabolites characteristic of intact plants and the possibility of modulating their biosynthesis by different exogenous influences. The accumulation of various specialized metabolites, such as picrosides, phenylpropanoids (rosmarinic, caffeic and chicoric acid) and flavonoids (rutin, isoquercetin, cyanidin, peonidin), was noted for various in vitro plant cultures [28,29]. Tea calli also retained the ability to accumulate PCs, including flavanols, which are characteristic of the original explants [30].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture

Zubova,

Goncharuk,

Nechaeva

et al. 2023

Preprint

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Tea plants (Camellia sinensis L.) are economically important horticultural crops for industrial use. The drink (tea) obtained from them is used all over the world, and its functional activity is determined by the polyphenols content and composition. Its accumulation is regulated by the action of many environmental factors. This paper presents data on the different intensity (50, 75, and 100 µmol·m−2·s−1) and duration of light exposure on tea plant in vitro cultures. Changes in morphophysiological characteristics, accumulation of carbohydrates and polyphenols, including their monomeric (phenylpropanoids, flavanols) and oligomeric (proanthocyanidins) forms, were studied. The malondialdehyde and photosynthetic pigments (chlorophyll a and b) contents were also analyzed. In the calli grown in light conditions, chloroplasts and anthocyanoplasts formation was noted, which was especially pronounced under the highest illumination intensity. A significant similarity between the growth of callus cultures, the carbohydrates and polyphenols accumulation at 50 and 75 µmol·m−2·s−1 was established. Which wasn’t characteristic of the calli grown at 100 µmol·m−2·s−1. The stress reaction absence in tea callus cultures has been demonstrated, which may be a consequence of the phenolic antioxidants’ accumulation in them. The obtained data evidence that their potential ability to accumulate primary (carbohydrates) and secondary (polyphenols) metabolites depends on the light exposure intensity and duration.

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Section: Introductionmentioning

confidence: 99%