Growth and flavonol accumulation of Ginkgo biloba leaves affected by red and blue light

Wang, Gaiping; Zhang, Lei; Wang, Guibin; Cao, Fuliang

doi:10.1016/j.indcrop.2022.115488

Cited by 14 publications

(4 citation statements)

References 59 publications

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“…The presence of five G-box elements in the FtUFGT163 promoter (Figure A) further supported the potential interaction with G subfamily FtbZIPs. Under various lighting conditions, particularly under blue light, bZIP transcription factors have been shown to effectively enhance the accumulation of flavonoid compounds, , aligning with the biological function of FtUFGT163 . Therefore, we performed a correlation analysis between the expression of FtbZIPs and the FtUFGT163 gene based on the blue light transcriptome (Figure S7 of the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Regulatory Module FtMYB5/6–FtGBF1–FtUFGT163 Promotes Rutin Biosynthesis in Tartary Buckwheat

Zhao,

Hu,

Fang

et al. 2024

J. Agric. Food Chem.

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Tartary buckwheat is highly valued for its abundant rutin (quercetin 3-O-rutinoside). As a flavonoid glycoside, rutin is synthesized with the crucial involvement of UDP-dependent glycosyltransferases (UGTs). However, the functions and transcriptional regulation of the UGT-encoded genes remain poorly understood. This study identified a key gene, FtUFGT163, potentially encoding flavonol 3-O-glucoside (1 → 6) rhamnosyltransferase in Tartary buckwheat through omics analysis and molecular docking methods. The recombinant FtUFGT163 expressed in Escherichia coli demonstrated the capacity to glycosylate isoquercetin into rutin. Overexpression of FtUFGT163 significantly enhanced the rutin content in Tartary buckwheat. Further investigation identified a novel bZIP transcription factor, FtGBF1, that enhances FtUFGT163 expression by binding to the G-box element within its promoter, thereby augmenting rutin biosynthesis. Additional molecular biology experiments indicated that the specific positive regulator of rutin, FtMYB5/6, could directly activate the FtGBF1 promoter. Collectively, this study elucidates a novel regulatory module, termed "FtMYB5/6−FtGBF1−FtUFGT163", which effectively coordinates the biosynthesis of rutin in Tartary buckwheat, offering insights into the genetic enhancement of nutraceutical components in crops.

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Section: Resultsmentioning

confidence: 99%

Regulatory Module FtMYB5/6–FtGBF1–FtUFGT163 Promotes Rutin Biosynthesis in Tartary Buckwheat

Zhao,

Hu,

Fang

et al. 2024

J. Agric. Food Chem.

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“…For example, the use of LED lights to apply red, mixed (red:blue = 1:1), and blue light to treat annual ginkgo seedlings showed that mixed and blue light significantly increased the flavonoid content. In particular, blue light significantly increased the flavonoid yield (0.76-fold increase) and antioxidant capacity, which laid a foundation for the exploitation of light quality in the cultivation of ginkgo leaf utilization forests [73]. The expression of the PAL, CHS, F3H, and FLS genes related to ginkgo flavonoid biosynthesis decreases with an increasing degree of shading [74].…”

Section: Ecological Factors 621 Lightmentioning

confidence: 98%

Overview and Recent Progress on the Biosynthesis and Regulation of Flavonoids in Ginkgo biloba L.

Guo,

Wang,

et al. 2023

IJMS

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Flavonoids and their derivatives play important roles in plants, such as exerting protective activity against biotic and abiotic stresses, functioning in visual signaling to attract pollinators, and regulating phytohormone activity. They are also important secondary metabolites that are beneficial to humans. Ginkgo biloba L. is a well-known relict plant considered to be a “living fossil”. Flavonoids present in ginkgo leaves have antioxidant and anti-aging capacities and show good therapeutic effects on a variety of neurological diseases. To date, studies on flavonoids have mainly focused on their extraction, pharmacological effects, and component analysis and on the expression levels of the key genes involved. However, a systematic review summarizing the biosynthesis and regulatory mechanisms of ginkgo flavonoids is still lacking. Thus, this review was conducted to comprehensively introduce the biological characteristics, value, and utilization status of ginkgo; summarize the effects, biosynthetic pathways, and transcriptional regulation of flavonoids; and finally, discuss the factors (ecological factors, hormones, etc.) that regulate the biosynthesis of flavonoids in ginkgo. This review will provide a reference basis for future research on the biosynthesis and efficient utilization of flavonoids in ginkgo.

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“…The quercetin, kaempferol, and isorhamnetin contents were determined using high-performance liquid chromatography (HPLC) (Alliance e2695, Waters, Milford, MA, USA), following the method outlined by Zhang et al [27]. The contents of quercetin, kaempferol, and isorhamnetin were calculated based on the peak areas in the liquid chromatograms.…”

Section: Determination Of the Flavonol Contentmentioning

confidence: 99%

Effects of UVA on Flavonol Accumulation in Ginkgo biloba

Zhao,

Wang,

Wang

et al. 2024

Forests

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Ginkgo is an economic tree species with high medicinal value, and flavonols are its main medicinal components. This research was conducted to investigate the molecular mechanism underlying the influence of Ultraviolet A (UVA) treatment on the synthesis of ginkgo flavonols with the aim of increasing their content. Ginkgo full-sib hybrid offspring were used as test materials. The phenylalanine ammonialyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarate: CoA ligase (4CL) enzyme activities, as well as flavonol contents, were measured under the same intensity of white light (300 μmol·m−2·s−1) with the addition of 20, 40, and 60 μmol·m−2·s−1 UVA separately after 20 days of treatment. The control check (CK) and treatment with the highest flavonol content were chosen for transcriptome sequencing analysis. The results showed that the PAL, C4H, and 4CL enzyme activities, as well as the flavonol and totalflavonol glycoside contents, of ginkgo hybrid progeny differed significantly under different UVA treatments. They showed a tendency to increase and then decrease, reaching a maximum value under UVA-4 (40 μmol·m−2·s−1 ultraviolet UVA light intensity) treatment. Ribonucleic acid (RNA) sequencing revealed the presence of 4165 genes with differential expression, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the metabolic pathways commonly enriched across all four comparison groups included ‘phenylpropanoid biosynthesis’, while the pathways commonly enriched in green-leaf ginkgo UVA-4 treatment (TL), yellow-leaf ginkgo mutant CK treatment (CKY), and green-leaf ginkgo CK treatment (CKL) were related to ‘flavonoid biosynthesis’. Treatment with UVA light led to the increased expression of PAL and 4CL enzymes in the phenylpropanoid biosynthesis pathway, as well as increased expression of chalcone synthase (CHS), Flavanone 3-hydroxylase (F3H), and flavonol synthase (FLS) enzymes in the flavonoid biosynthesis pathway, thereby promoting the synthesis of ginkgo flavonols. In summary, the use of 40 μmol·m−2·s−1 UVA treatment for 20 days significantly increased the flavonol content and the expression of related enzyme genes in ginkgo hybrid offspring, enhancing ginkgo flavonoids and increasing the medicinal value of ginkgo.

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Growth and flavonol accumulation of Ginkgo biloba leaves affected by red and blue light

Cited by 14 publications

References 59 publications

Regulatory Module FtMYB5/6–FtGBF1–FtUFGT163 Promotes Rutin Biosynthesis in Tartary Buckwheat

Regulatory Module FtMYB5/6–FtGBF1–FtUFGT163 Promotes Rutin Biosynthesis in Tartary Buckwheat

Overview and Recent Progress on the Biosynthesis and Regulation of Flavonoids in Ginkgo biloba L.

Effects of UVA on Flavonol Accumulation in Ginkgo biloba

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