Growth and Extracellular Carbonic Anhydrase Activity of Zooxanthellae Symbiodinium sp. in Response of Zinc Enrichment

Karim, Widiastuti; Kaswadji, Richardus; Prartono, Tri; Gorettipanggabean, Lily Maria

doi:10.4308/hjb.18.4.157

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…The ensuing elevation of the CO 2 /O 2 ratio increases its efficiency as carboxylase (Giordano et al, 2005 ). Extracellular carbonic anhydrase (eCA) has been detected in several phototrophic microorganisms, both in cyanobacteria and in eukaryotes, and is thought to have a role in DIC uptake into the cell (Katsunori and Shigetoh, 1986 ; Nimer et al, 1999 ; Kupriyanova et al, 2003 , 2007 , 2011 ; Karim et al, 2011 ; Hopkinson et al, 2013 ; Hamizah et al, 2017 ). This may be increasingly important at higher pH and/or lower dissolved free CO 2 concentrations (i.e., hyperalkaline environments).…”

Section: Introductionmentioning

confidence: 99%

“…This may be increasingly important at higher pH and/or lower dissolved free CO 2 concentrations (i.e., hyperalkaline environments). So far, studies on the role of iCA and eCA in photosynthesis focused on phytoplankton rather than biofilms/mats (Katsunori and Shigetoh, 1986 ; Nimer et al, 1999 ; Kupriyanova et al, 2003 , 2007 , 2011 ; Karim et al, 2011 ; Hopkinson et al, 2013 ; Hamizah et al, 2017 ). Due to mass transfer resistances, the pH value in mats can be very high during photosynthesis, likely enhancing the role of eCA for DIC uptake and photosynthesis (Revsbech et al, 1981 ; Revsbech and Jørgensen, 1983 ; Revsbech, 1989 ; Jensen et al, 1993 ; Epping et al, 1999 ; Ionescu et al, 2014 ; Nielsen et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

Sharp

Ataeian

et al. 2018

Front. Microbiol.

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Alkaline Soda Lakes are extremely productive ecosystems, due to their high dissolved inorganic carbon (DIC) concentrations. Here, we studied the dynamics of the carbonate system, in particular, the role of extracellular carbonic anhydrase (eCA) of an alkaliphilic phototrophic biofilm composed of bacteria enriched from soda lake benthic mats. By using measurements with microsensors and membrane inlet mass spectrometry, combined with mathematical modeling, we show how eCA controls DIC uptake. In our experiments, the activity of eCA varied four-fold, and was controlled by the bicarbonate concentration during growth: a higher bicarbonate concentration led to lower eCA activity. Inhibition of eCA decreased both the net and the gross photosynthetic productivities of the investigated biofilms. After eCA inhibition, the efflux of carbon dioxide (CO2) from the biofilms increased two- to four-fold. This could be explained by the conversion of CO2, leaking from cyanobacterial cells, by eCA, to bicarbonate. Bicarbonate is then taken up again by the cyanobacteria. In suspensions, eCA reduced the CO2 leakage to the bulk medium from 90 to 50%. In biofilms cultivated at low bicarbonate concentration (~0.13 mM), the oxygen production was reduced by a similar ratio upon eCA inhibition. The role of eCA in intact biofilms was much less significant compared to biomass suspensions, as CO2 loss to the medium is reduced due to mass transfer resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

Sharp

Ataeian

et al. 2018

Front. Microbiol.

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“…Zn is also required by the carbonic anhydrase enzyme the help in interconversion of carbonic acid and carbon dioxide in C4 pathway of photosynthesis 5 . Zn increase the carbonic anhydrage activity ten folds in algage also 6 . Zn shortage result in many physiological problem of plants 7 .…”

Section: Introductionmentioning

confidence: 79%

Comparative Efficacy of Zn Supplement and Zinc Oxide Nanoparticles Over the Seed Germination of Lentil and Chick Pea

Choudhary¹,

Khandelwal²

2020

J. Pure Appl. Microbiol.

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The mineral nutrients are absorb from the soil by the roots, these are either macro or micro nutrients. Zinc is micro nutrients that help in several physiological process of plants and help in phyto hormone synthesis. The present Study includes the synthesis of ZnOPs by using Zinc oxalate decomposing method and these particles are characterized by UV-vis spectroscopy, and size was analyzed by Zeta analyzer. ZnOPs size was analyzed 65 nm. Seed germination was done by paper towel method. Soaking of seed was done in 100ppm, 500ppm and 1000ppm of ZnOPs and 100 ppm of ZnO supplement solution. After 5 day germination, germination percentage and seed vigor index (SVI) was calculated. It was observed that seed germination at 100 ppm is maximum in both the seed of Lentil (Lens esculentum Linn) and Chick pea (Cicer arietinum Linn). Seed vigor index was found increased at concentration of 100ppm. Presented study inferred that zinc provided as nanoparticle absorb more prominently than the traditional zinc supplement available in market at low concentration as the ZnOPs concentration increases the germination and seed vigor index retarded so it can be proposed that micronutrient in nano particles based formulation at lower concentration can provide better result in agriculture production.

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“…We identified zinc (Zn) and nitrate (

{NO}_{3}^{‐}

) transporters as potential CWPs. Both Zn and

{NO}_{3}^{‐}

are used by the dinoflagellate cell in several biological functions, ranging from cell growth to metabolism (Karim et al, 2011) and must be acquired from the surrounding environment. The presence of Zn induced facilitator‐like‐1a and high affinity

{NO}_{3}^{‐}

transporters has been previously shown in plants (Lezhneva et al, 2014; Ricachenevsky et al, 2011), so their presence is not surprising in the cell wall of Symbiodiniaceae.…”

Section: Resultsmentioning

confidence: 99%

Cell wall proteomic analysis of the cnidarian photosymbionts Breviolum minutum and Cladocopium goreaui

Tortorelli

Oakley

Davy

et al. 2021

J Eukaryotic Microbiology

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The algal cell wall is an important cellular component that functions in defense, nutrient utilization, signaling, adhesion, and cell–cell recognition—processes important in the cnidarian–dinoflagellate symbiosis. The cell wall of symbiodiniacean dinoflagellates is not well characterized. Here, we present a method to isolate cell walls of Symbiodiniaceae and prepare cell‐wall‐enriched samples for proteomic analysis. Label‐free liquid chromatography–electrospray ionization tandem mass spectrometry was used to explore the surface proteome of two Symbiodiniaceae species from the Great Barrier Reef: Breviolum minutum and Cladocopium goreaui. Transporters, hydrolases, translocases, and proteins involved in cell‐adhesion and protein–protein interactions were identified, but the majority of cell wall proteins had no homologues in public databases. We propose roles for some of these proteins in the cnidarian–dinoflagellate symbiosis. This work provides the first proteomics investigation of cell wall proteins in the Symbiodiniaceae and represents a basis for future explorations of the roles of cell wall proteins in Symbiodiniaceae and other dinoflagellates.

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Growth and Extracellular Carbonic Anhydrase Activity of Zooxanthellae Symbiodinium sp. in Response of Zinc Enrichment

Cited by 4 publications

References 37 publications

Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

Role of Extracellular Carbonic Anhydrase in Dissolved Inorganic Carbon Uptake in Alkaliphilic Phototrophic Biofilm

Comparative Efficacy of Zn Supplement and Zinc Oxide Nanoparticles Over the Seed Germination of Lentil and Chick Pea

Cell wall proteomic analysis of the cnidarian photosymbionts Breviolum minutum and Cladocopium goreaui

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