Growth and extended survival ofEscherichia coliO157:H7 in soil organic matter

Abstract

Enterohaemorrhagic Escherichia coli such as serotype O157:H7 are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection. E. coli is able to grow in the tissue of food plants such as spinach. While fecal contamination is the primary suspect, soil has been underestimated as a potential reservoir. Persistence of bacterial populations in open systems is the product of growth, death, predation, and competition. Here we report that E. coli O157:H7 can grow using the soluble compounds in soil, and characterize the effect of soil growth in the stationary phase proteome.

Attachment and colonization of Salmonella on ‘Rayada’, ‘Golden Delicious’, and ‘Red Delicious’ apples

“…The soil used as a nutrient source in our study was a regosol calcareous soil with a clay texture . Recently, E. coli O157:H7 growth has been reported at 30 °C using water‐soluble organic matter from different types of soils, including forest, corn field and garden soil . In the present study, the maximum increase in the population was seen with S .…”

The Diversity of a Polyclonal FluCell‐SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosa

“…[31] Growth conditions like the mediumc omposition, temperature or growth phase significantly influence the membrane proteome also when Gram-negative bacteria are cultivated in vitro. [32,33] When cultivated in ac omplex medium P. aeruginosa shows the typical bacterial growth phases including the lag-, exponential growth, early stationary and stationaryp hases ( Figure 4A)r epresented by characteristicc hanges in the growth rates ( Figure 4B). Both, R16 and C1R1 could label cells isolatedf rom all growth phases, however,f luorescence signals of R16 where particularly higher compared to C1R1.…”