Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids.

Oyaizu, Hiroshi; Komagata, Kazuo

doi:10.2323/jgam.29.17

Cited by 203 publications

(160 citation statements)

References 19 publications

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“…The quinone systems of the representative strains, ubiquinone Q-8 of the strains affiliated with Comamonas or Stenotrophomonas, menaquinone MK-6 of the Chryseobacterium strain, and ubiquinone Q-9 of the Acinetobacter or Pseudomonas affiliated strains were in complete agreement with the traits reported for representatives of the genus (Collins and Jones 1981;Oyaizu and Komagata 1983;Busse et al 1989;Yokota et al 1992;Kämpfer et al 2003;Hauser et al 2004;Stolz et al 2007).…”

Section: Discussionsupporting

confidence: 77%

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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-This study was conducted to improve the knowledge about bacteria associated with honey bees, Apis mellifera carnica. In this survey, the diversity of Gram-negative non-fermenting bacteria isolated and cultivated from pollen loads, honey sac, freshly stored nectar, and honey was investigated. Bacteria were characterized by a polyphasic approach. Based on morphological and physiological characteristics and comparison of isolates protein patterns after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 11 protein similarity groups were established and confirmed by enterobacterial repetitive intergenic consensus PCR. One isolate, representing a protein similarity group (representative strain), was characterized in more detail by analysis of respiratory quinones, amplified ribosomal DNA restriction analysis, and 16S rDNA sequence analysis. Based on the results of these examinations, seven representative strains were identified as members of the genus Pseudomonas. The remaining representative strains were allocated to the genera Acinetobacter, Chryseobacterium, Stenotrophomonas, and Commamonas.bacterial diversity / PCR / honey / honey bees / 16S rDNA gene

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Section: Discussionsupporting

confidence: 77%

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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“…Bacteria of the genus Pseudomonas belong to the Gammaproteobacteria and are aerobic, chemoorganotrophic, non-spore-forming, motile, Gram-negative rods with polar flagella and a respiratory rather than a fermentative metabolism (Palleroni, 1984;Timmis, 2002). Over the years, species of the genus have been reclassified and revised several times (Sneath et al, 1981;Oyaizu & Komagata, 1983;Vancanneyt et al, 1996;Anzai et al, 2000). Members of the genus Pseudomonas are widely distributed in soil, water, air, plants and many other natural habitats (Palleroni, 1992).…”

mentioning

confidence: 99%

Pseudomonas bauzanensis sp. nov., isolated from soil

Zhang

Liu

Zhou

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, aerobic, motile rod, designated BZ93 T , was isolated from soil from an industrial site. The strain grew at 5-30 6C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93 T was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2 T (97.8 % 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163 T (97.4 %). The predominant cellular fatty acids of strain BZ93 T were C 18 : 1 v7c (54.8 %), summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH; 10.3 %), C 16 : 0 (9.9 %) and C 17 : 0 cyclo (7.4 %). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93 T (5DSM 22558 T 5CGMCC 1.9095 T 5LMG 26048 T ).The genus Pseudomonas was originally created by Migula (1894). Bacteria of the genus Pseudomonas belong to the Gammaproteobacteria and are aerobic, chemoorganotrophic, non-spore-forming, motile, Gram-negative rods with polar flagella and a respiratory rather than a fermentative metabolism (Palleroni, 1984;Timmis, 2002). Over the years, species of the genus have been reclassified and revised several times (Sneath et al., 1981;Oyaizu & Komagata, 1983;Vancanneyt et al., 1996;Anzai et al., 2000). Members of the genus Pseudomonas are widely distributed in soil, water, air, plants and many other natural habitats (Palleroni, 1992). Some representatives of this genus colonize cold habitats (Yumoto et al., 2001; Reddy et al., 2004;Bozal et al., 2007;Margesin et al., 2008). The present study deals with the characterization of a novel strain of the genus Pseudomonas that was isolated from soil and is able to grow at low temperatures. Strain BZ93 T was isolated from soil from an industrial site (containing high amounts of heavy oil and heavy metals) in Bozen, South Tyrol, Italy, as described previously (Zhang et al., 2010). The soil was sampled and processed under sterile conditions in spring 2008. A sample (10 g) was shaken with 90 ml sterile 1 % sodium pyrophosphate for 20 min at 150 r.p.m. Appropriate dilutions were prepared with sterile 0.9 % NaCl and 0.1 ml aliquots were spread on R 2 A agar (0.05 % yeast extract, 0.05 % peptone, 0.05 % Casamino acids, 0.05 % glucose, 0.05 % starch, 0.03 % sodium pyruvate, 0.03 % K 2 HPO 4 , 0.005 % MgSO 4 , 1.5 % agar; pH 7; Reasoner & Geldreich, 1985) and incubated at 20 u C. A white colony was purified and designated BZ93 T . Strain BZ93 T was routinely cultured on R 2 A medium at 20 u C and maintained as a suspension in skimmed milk (10 % w/v) at 280 u C. Pseudomonas pertucinogena LMG 1874 T and Pseudomonas xiamenensis CGMCC 1.6446 T were routinely grown on R 2 A agar at 25 u C and used as reference strains.DNA was extracted and purified as described b...

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“…Most strains contained mainly 16-carbon (16:0), unsaturated 18-carbon (18:1) and 18-carbon (18:0) fatty acids. Strains were classified based on the presence of 2-or 3-hydroxy acids, although these fatty acids usually accounted for less than 10% of the total 13) . Some strains (P5-24, P5-29, P5-31, G-14) contained a significant amount of 2-OH 14:0.…”

Section: Quinone and Cellular Fatty Acid Compositionsmentioning

confidence: 99%

Phylogenetic Analysis of Bacterial Populations Found in Groundwater and an Acidic Stream Draining from an Abandoned Coal Mine.

2001

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A phylogenetic analysis of heterotrophic bacterial populations inhabiting streams and groundwater contaminated by acid mine drainage (AMD) was conducted. The samples were collected from sites around an inactive underground Dae-Sung coal mine at Keumsan, Korea. The investigation showed pigment-forming bacteria to be the major strains inhabiting the pollutant, accounting for up to approximately 50% of all isolates. Twenty-six pigment-forming bacteria were isolated and their taxonomic characteristics determined by phenotypic, chemotaxonomic and phylogenetic analyses. Based on a phylogenetic analysis using 16S ribosomal RNA gene nucleotide sequences, these isolates were found to fall within four major phylogenetic groups: a, b, and g subdivisions of Proteobacteria; and low-G+C gram-positive bacteria. The a-Proteobacteria were further separated into a-1, a-2 and a-4 subclasses. Many isolates from the polluted stream (site P5) and groundwater (site G) were identified as Sphingomonas of the Proteobacteria a-4 subclass. Because strains P5-21 and P5-11 appeared to be novel species within the genus Sphingomonas, the discussion was focused on their taxonomy as well as abundance in the polluted regions.

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Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids.

Cited by 203 publications

References 19 publications

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Pseudomonas bauzanensis sp. nov., isolated from soil

Phylogenetic Analysis of Bacterial Populations Found in Groundwater and an Acidic Stream Draining from an Abandoned Coal Mine.

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