Group-specific detection of 2-deoxystreptamine aminoglycosides in honey based on antibodies against ribostamycin

Galvidis, Inna A.; Burkin, Konstantin M.; Eremin, Sergei A.; Burkin, Maksim A.

doi:10.1039/c9ay01372e

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…Alternatively, a one-step coating could be carried out using a mixture of SAV and B 7 –hapten previously prepared in PBS and coated overnight. The plates with the immobilized hapten were then washed and used for a conventional indirect competitive ELISA based on coated hapten–protein conjugates using specific rabbit anti-hapten antibodies and GAR–HRP [ 28 ]. Antibodies added to the wells in the working concentration in 1% BSA-PBST (0.1 mL) were incubated for 1 h on an ST-3 L plate shaker (ELMI Ltd., Latvia) at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Burkin

Galvidis

Eremin

2022

Foods

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(Strept)avidin–biotin technology is frequently used in immunoassay systems to improve their analytical properties. It is known from clinical practice that many (strept)avidin–biotin-based tests provide false results when analyzing patient samples with a high content of endogenous biotin. No specific investigation has been carried out regarding possible interferences from avidin (AVI) and biotin (B7) contained in food matrices in (strept)avidin–biotin-based immunoanalytical systems for food safety. Two kinds of competitive ELISAs for bacitracin (BT) and colistin (COL) determination in food matrices were developed based on conventional hapten–protein coating conjugates and biotinylated BT and COL bound to immobilized streptavidin (SAV). Coating SAV–B7–BT and SAV–B7–COL complexes-based ELISAs provided 2- and 15-times better sensitivity in BT and COL determination, corresponding to 0.6 and 0.3 ng/mL, respectively. Simultaneously with the determination of the main analytes, these kinds of tests were used as competitive assays for the assessment of AVI or B7 content up to 10 and 1 ng/mL, respectively, in food matrices (egg, infant milk formulas enriched with B7, chicken and beef liver). Matrix-free experiments with AVI/B7-enriched solutions showed distortion of the standard curves, indicating that these ingredients interfere with the adequate quantification of analytes. Summarizing the experience of the present study, it is recommended to avoid immunoassays based on avidin–biotin interactions when analyzing biosamples containing these endogenous factors or enriched with B7.

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Section: Methodsmentioning

confidence: 99%

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Burkin

Galvidis

Eremin

2022

Foods

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“…The specificity was estimated by the cross-reactivity (CR) of related polyenes and aminoglycoside-bearing antibacterials [ 18 ] as the percentage IC 50 NAT /IC 50 ANALOG.…”

Section: Methodsmentioning

confidence: 99%

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

2022

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The antifungal drug natamycin (NAT) is widely used in medicine and in the food industry as preservative E235 for a wide variety of foods. The risk of the development of resistance to NAT and its spread in relation to other polyene antibiotics is fraught with the emergence of incurable infections. This work is devoted to the development of an immunoassay to investigate the prevalence of NAT use for food preservation. Two immunogen designs based on tetanus toxoid, conjugated to NAT through different sites of hapten molecules, were compared in antibody generation. Assay formats using heterologous coating antigens were superior for both antibodies. The ELISA variant demonstrated the highest sensitivity (IC50 = 0.12 ng/mL), and a limit of detection of 0.02 ng/mL was selected for NAT determination. The optimized extraction procedure provided a recovery rate of 72–106% for various food matrixes with variations below 12%. Cyclodextrins, as well as NAT–cyclodextrin complex formulations, showed no interference with the quantification of NAT. One hundred and six food product brands, including baked goods, wines, beers, drinks, sauces, and yogurts, were tested to assess the prevalence of the undeclared use of NAT as a preservative. The screening examination revealed three positive yogurts with an undeclared NAT incorporation of 1.1–9.3 mg/kg.

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Group-specific detection of 2-deoxystreptamine aminoglycosides in honey based on antibodies against ribostamycin

Abstract: Antibodies against the common 2-desoxystreptamine fragment of aminoglycosides are capable of revealing the wide spectrum of analytes in honey at a 10 μg kg−1 level.

Cited by 2 publications

References 34 publications

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Influence of Endogenous Factors of Food Matrices on Avidin—Biotin Immunoassays for the Detection of Bacitracin and Colistin in Food

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

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