GroEL to DnaK chaperone network behind the stability modulation of σ<sup>32</sup> at physiological temperature in <i>Escherichia coli</i>

Patra, Monobesh; Roy, Sourav; Dasgupta, Rakhi; Basu, Tarakdas

doi:10.1016/j.febslet.2015.10.034

Cited by 14 publications

(5 citation statements)

References 39 publications

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“…The experiment was done according to the protocol of [34]. NP-treated cells (seeded at 1×10 6 cells/65 mm Petri-plate) were washed three timeswith ice-cold PBS and finally suspended in 600 μl ice-cold 1X RIPA lysis buffer (abcam, Cambridge, UK) supplemented with protease inhibitor cocktail (abcam).…”

Section: Detection Of Caspase-3 By Thewestern Blot Methodmentioning

confidence: 99%

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

Chakraborty

Basu

2017

Nanotechnology

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In two earlier communications (Chatterjee et al 2012 Nanotechnology 23 085103, Chatterjee et al 2014 Nanotechnology 25 135101), we reported the development of a simple and unique method of synthesizing highly stable metallic copper nanoparticles (Cu NPs) with high antibacterial activity. Here we report on the cytotoxic potency of the NPs against cancer cells. The value of the IC50 dose of the Cu NPs against human skin cancer cell A-375 was found to be 1.71 μg ml only, which was much less than values reported so far, and this concentration had no cytotoxic effect on normal white blood cells. The NPs caused (i) lowering of cell membrane rigidity, (ii) DNA degradation, (iii) chromosomal condensation, (iv) cell cycle arrest in the G2/M phase, (v) depolarization of the mitochondrial membrane and (vi) apoptosis of cells. Cellular apoptosis occurred in the caspase-9-mediated intrinsic pathway. This study revealed that our Cu NPs had high anticancer properties by killing tumor cells through the apoptotic pathway. Since this particle has high antibacterial activity, our Cu NPs might be developed in future as a dual action drug-anticancer as well as antibacterial.

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Section: Detection Of Caspase-3 By Thewestern Blot Methodmentioning

confidence: 99%

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

Chakraborty

Basu

2017

Nanotechnology

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“…This does not necessarily require, however, that both chaperones act in a defined order to fold proteins, as DnaK may backup post-translationally if TF does not sufficiently function cotranslationally. Additionally, DnaK can cooperate bidirectionally with the chaperonin GroEL/ES, which is also an ATP-controlled chaperone [94][95][96].…”

Section: Box 1 Pqc: Maintaining Proteostasis In the Cellmentioning

confidence: 99%

The Hsp70–Hsp90 Chaperone Cascade in Protein Folding

Luengo

Mayer

Rüdiger

2019

Trends in Cell Biology

189

140

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“…At 44 °C, ramFexpressing cells grew identically to control cells (P=0.18, t-test), whereas at 30 °C ramF expression caused a severe growth reduction (P=1.27x10 -5 , t-test). This temperature-dependent phenotype further indicated that ramF activity may depend on protein homeostasis pathways as chaperone levels are often temperature-dependent [35][36][37][38][39][40][41] .…”

Section: Ramf Interacts With Chaperones To Remodel Protein Homeostasi...mentioning

confidence: 95%

Selection of ade novogene that can promote survival ofE. coliby modulating protein homeostasis pathways

Frumkin

Laub

2023

Preprint

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Cells sometime adapt to challenging environments by turning non-functional loci into functional genes in a process termedde novogene birth. But how proteins with random amino acid sequences integrate into existing cellular pathways to provide a benefit remains poorly understood. Here, we screened ~108random genes for their ability to rescue growth arrest ofE. colicells producing the ribonuclease toxin MazF. Approximately 2,000 random genes could promote growth by reducing transcription from the promoter drivingmazFexpression. Additionally, one gene, namedrandomantitoxin ofMazF(ramF), whose protein product was well-tolerated in cells and neutralized MazF by interacting with chaperones, leading to MazF proteolysis. We show that the specificity of RamF for MazF relative to other toxins relies on the degron-like function of MazF's first 10 amino acids. Finally, we demonstrate that random proteins can improve during evolution by identifying beneficial mutations that turned RamF into a more efficient inhibitor. Our work provides a mechanistic basis for howde novogene birth can produce new, functional proteins that are integrated into complex cellular systems and provide a benefit to cells.

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GroEL to DnaK chaperone network behind the stability modulation of σ³² at physiological temperature in Escherichia coli

Cited by 14 publications

References 39 publications

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

The Hsp70–Hsp90 Chaperone Cascade in Protein Folding

Selection of ade novogene that can promote survival ofE. coliby modulating protein homeostasis pathways

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GroEL to DnaK chaperone network behind the stability modulation of σ32 at physiological temperature in Escherichia coli

Cited by 14 publications

References 39 publications

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

Metallic copper nanoparticles induce apoptosis in a human skin melanoma A-375 cell line

The Hsp70–Hsp90 Chaperone Cascade in Protein Folding

Selection of ade novogene that can promote survival ofE. coliby modulating protein homeostasis pathways

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Product

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GroEL to DnaK chaperone network behind the stability modulation of σ³² at physiological temperature in Escherichia coli