Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase

Kuzuhara, Takashi; Iwai, Yoshio; Takahashi, Hironobu; Hatakeyama, Dai; Echigo, Noriko

doi:10.1371/currents.rrn1052

Cited by 101 publications

(94 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The documented anti-viral activities of EGCG include the inhibition of the influenza virus, 18) human immunodeficiency virus type 1 (HIV-1), 19,20) Epstein-Barr virus, 21,22) herpes simplex virus, 23) and hepatitis B virus. 24) In vitro, EGCG inhibited various viral enzymes, such as adenovirus protease adenain, 25) influenza A RNA polymerase, 26) and HIV-1 integrase. 27) EGCG bound to CD4 on T cells and prevented its interaction with HIV gp120.…”

Section: Discussionmentioning

confidence: 99%

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Fukazawa

Suzuki

Wakita

et al. 2012

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

We describe a cell-based, microplate colorimetric screen for anti-hepatitis C virus (HCV) drugs that exploits the HCV-JFH1 viral culture system. Antiviral activity was assessed by measuring protection against the HCV-JFH1-induced cytopathic effect (CPE) in Huh7.5.1 cells using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) viability assay. The use of serum-free medium substantially sensitized Huh7.5.1 cells to HCV-induced CPE, causing sufficient cell death to perform colorimetric assays for anti-HCV activity in 96-well plates. As a proof of concept, we carried out a pilot screen of an inhibitor library and identified cyclosporin A and tamoxifen, two compounds with reported anti-HCV activity. Using the assay, we discovered the anti-HCV properties of the plant flavonoids epigallocatechin gallate (EGCG) and 7,8-benzoflavone (α-naphthoflavone). Other gallate-type catechins and flavones also displayed anti-HCV activity, but 5,6-benzoflavone (β-naphthoflavone), flavanone, and non-gallate catechins were inactive. EGCG apparently acted mainly on HCV entry, although it may also block other steps. In contrast, 7,8-benzoflavone was presumed to inhibit later stages of the HCV life cycle. This assay is simple, reliable and cost-effective; does not require any specially engineered cell lines or viruses; and should be useful in the identification of compounds with anti-HCV activity. Key words hepatitis C virus; 7,8-benzoflavone (α-naphthoflavone); epigallocatechin gallateMore than 170 million people worldwide are chronically infected with the hepatitis C virus (HCV) and are at risk for developing liver diseases such as cirrhosis and hepatocellular carcinoma. Vaccines against HCV are not currently available; furthermore, the standard interferon/ribavirin combination therapy is not effective in approximately half of HCV-infected patients, and it has considerable side effects.1,2) Thus, there is an obvious and urgent need for new agents that can enhance or replace current HCV therapies.Screening programs using HCV replicon-based systems have successfully identified compounds that act on HCV RNA replication. However, replicon systems do not reproduce the entire HCV life cycle, and they cannot isolate inhibitors of many important steps such as viral entry, assembly, and egress. HCV cell culture infection models that recapitulate the entire viral life cycle in vitro have greatly enhanced the opportunity for HCV drug discovery. Several reports, including ours, have demonstrated that this model can overcome the limitations of the HCV replicon system and enable the discovery of compounds that target various stages of the HCV life cycle. [3][4][5][6][7][8] Using the HCV cell culture system, we previously developed a tube-capture-reverse transcription-polymerase chain reaction (RT-PCR) assay for screening HCV inhibitors and identified bisindolylmaleimides and indolocarbazoles as inhibitors of HCV replication.4) Here, we describe another screening method for the detection of anti-HCV activity. This assay measures t...

show abstract

Section: Discussionmentioning

confidence: 99%

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Fukazawa

Suzuki

Wakita

et al. 2012

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Kizuhara and co-workers have reported that green tea catechins inhibited this viral endonuclease activity and that their galloyl group was important for their function [93]. Docking simulations revealed that catechins with galloyl group fitted well into the active pocket of the endonuclease domain to enable stable binding.…”

Section: Catechinsmentioning

confidence: 99%

Antioxidant Therapy as a Potential Approach to Severe Influenza-Associated Complications

2011

View full text Add to dashboard Cite

“…Much research has shown that plant extracts and natural products have a wide range of pharmacological activities, including anti-inflammatory, antibacterial, anticancer and antiviral [12]. For instance, polyphenols from green tea have the ability to inhibit influenza virus replication [19,20] and polyphenolic compounds derived from Phellinus baumii could inhibit influenza virus infection [10]. In recent years, the antiviral activity of some species of Paeonia has been explored.…”

Section: Uplc-q-tof-ms Analysismentioning

confidence: 99%

Anti-Influenza Virus Activity and Constituents Characterization of Paeonia delavayi Extracts

Yang

Huang

2016

Molecules

View full text Add to dashboard Cite

Paeonia delavayi, an endemic species in southwestern China, has been widely used as a traditional remedy for cardiovascular, extravasated blood, stagnated blood and female diseases in traditional Chinese medicine (TCM). However, there are no reports on the anti-influenza virus activity of this species. Here, the anti-influenza virus activity of P. delavayi root extracts was first evaluated by an influenza virus neuraminidase (NA) inhibition assay. Meantime, constituents in the active extracts were identified using ultra-high performance liquid coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and seven major identified constituents were used to further evaluate the NA inhibitory activity. The results showed that the ethyl acetate fraction (EA) and the ethanol fraction (E) of P. delavayi both presented strong NA inhibitory activity with IC 50 values of 75.932 µg/mL and 83.550 µg/mL, respectively. Twenty-seven constituents were characterized in these two active extracts by UPLC-Q-TOF-MS analysis, and seven major identified constituents exhibited high activity against the influenza virus.Among them, Benzoylpaeoniflorin (IC 50 = 143.701 µM) and pentagalloylglucose (IC 50 = 62.671 µM) exhibited the highest activity against the influenza virus, even far stronger than oseltamivir acid (IC 50 = 281.308 µM). This study indicated that P. delavayi was a strong NA inhibitor, but cell-based inhibition, anti-influenza virus activity in vivo and anti-influenza virus mechanism still need to be tested and explored.

show abstract

Green tea catechins inhibit the endonuclease activity of influenza A virus RNA polymerase

Cited by 101 publications

References 12 publications

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Antioxidant Therapy as a Potential Approach to Severe Influenza-Associated Complications

Anti-Influenza Virus Activity and Constituents Characterization of Paeonia delavayi Extracts

Contact Info

Product

Resources

About