Green Synthesis of Silver Nanoparticles from Aqueous Extract ofTinospora crispaStems Accelerate Wound Healing in Rats

Abstract

Tinospora crispa has been known as an herbal medicine with many biological activities. The current study evaluates silver nanoparticles in accelerating the rate of wound healing and closure of excision wounds in rats. A novel technique was followed to synthesize nanosilver particles, x-ray diffraction, UV–Vis's spectroscopy, and transition electron microscope were used. Sprague Dawley rats (24) were separated into 4 clusters: vehicle group (G1), treated with Vaseline; positive control (G2), treated with the intrasite gel; Experimental groups (G3 and G4), treated with 175 and 350 ppm silver nanoparticles, respectively, with an excisional wound created on the neck area. Wounds were treated topically twice daily for two weeks and sacrificed on day fifteenth. Histology, immunohistochemistry (expression of Bax and HSP 70 proteins), and Masson Trichrome staining procedures disclose that wounds dressed with silver nanoparticles exhibited wound closure site contained less scar, less inflammatory cells, and more collagen compared vehicle group. The endogenous antioxidant enzymes (SOD and CAT) and TGF-β were significantly increased and lipid peroxidation (MDA) and TNF-α were decreased in wound tissue homogenate dressed with silver nanoparticles compared to the vehicle cluster. Silver nanoparticles suggestively improved cell proliferation and stimulated the rate of wound closure, with less scarring, and more fibroblast. Graphical abstract [Formula: see text]

Gastroprotective effects of Polygonatum odoratum in rodents by regulation of apoptotic proteins and inflammatory cytokines

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Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms

“…After that, all rats received a small dose of xylazine and ketamine in 12.5 mg/kg, 87.5 mg/kg, respectively, and shaved their dorsal neck and disinfected with 70% alcohol. An equal cut precise cut (2.00 cm, diameter) was created on each rat's dorsal neck by using a round seal 32 (Figure 1). Then, the rats received different treatments on their dorsal neck twice daily: A, rats had topical treatment of normal saline (0.2 mL). B, rats had topical intrasite gel therapy (0.2 mL). C, rats had 0.2 mL of 250 mg/kg of CCS. D, rats had 0.2 mL of 500 mg/kg of CCS. …”

“…Sections (5 μm) of skin tissue were fixed on slides followed by a staining procedure utilizing Hematoxylin, Eosin, and Masson's trichrome). After incubation for overnight, the microscopic (Nikon) evaluation of slides performed for any possible histological alterations, deposition of collagen, inflammatory cell infiltration, proliferation of fibroblast, epithelialization, and neovascularization 32 …”

“…The separated supernatant evaluated for the amount of hydroxyproline (HXP) content by using a commercially available ELISA kit, according to the instrument catalog (Sigma Aldrich, China). The entire step‐by‐step procedure including precision, specificity, optimization, and reproducibility can be found elsewhere 32 …”

Sinapic Acid Attenuate Liver Injury by Modulating Antioxidant Activity and Inflammatory Cytokines in Thioacetamide-Induced Liver Cirrhosis in Rats

“…The slides were deepened in an ammonia container (0.037 M/L) ten times, and antigens were retrieved via microwave boiling in sodium citrate buffer (10 mM). The immunohistochemical stain-positive cells were detected via microscope observations [22,38]. Then, the percent calculation of positive cells was determined.…”