Green synthesis of silver nanoparticles by Nigella sativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidant effects

Abstract

Green synthesis of silver nanoparticles has gained great interest among scientists. In view of this data, we conducted this study to identify the ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa extract in diabetic neuropathy induced experimentally. In this study, 50 adult male albino rats were used and they were randomly divided into five groups; the first group was the healthy control group, the second group were the diabetic neuropathy diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and treated with silver nanoparticles, Nigella sativa extract and green synthesized silver nanoparticles using Nigella sativa extract respectively. Biochemical parameters including diabetic, inflammatory and antioxidant biomarkers were evaluated. Brain histopathology was also performed. Results revealed substantial rise in glucose, AGE, aldose reductase with insulin reduction in diabetic neuropathy induced group as compared to healthy control. Also, inflammatory markers increased significantly in diabetic neuropathy induced group. A remarkable change in oxidative status was observed in the same group. Furthermore, significant decline in nitrotyrosin level was observed. Regarding gene expression, we found significant down regulation in brain TKr A accompanied by upregulation of nerve growth factor in diabetic neuropathy group comparing with healthy control. Several treatments for diabetic neuropathy remarkably ameliorate all the investigated biomarkers. Histological findings are greatly relied on for the results achieved in this study. Therefore, it can be established that green synthesis of silver nanoparticles in combination with Nigella sativa extract could be a newly neuroprotective agents against inflammation and oxidative stress characterizing diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants effects.

Green Synthesis of Gold and Silver Nanoparticles from Plant Extracts and Their Possible Applications as Antimicrobial Agents in the Agricultural Area

“…In this approach, the aqueous fruit extract of the plant fulfilled reducing and stabilizing functions for the preparation, and the obtained AgNPs exhibited good catalytic and antibacterial activities [ 83 ]. Alkhalaf et al conducted a study to identify the effect of the green synthesis of AgNPs from a Nigella sativa plant extract, resulting in NPs that exhibit antioxidant activity [ 84 ].…”

Silver Nanoparticles: Mechanism of Action and Probable Bio-Application

“…In vitro, silver nanoparticles are also attributed with an anti-inflammatory effect by playing a role in the wound healing process due to TNF-α, interferons, and interleukin 1 as well as inhibition of COX-2 and MMP-3 expressions, and also have the potential to reduce the activity of TNF-α, which is involved in inflammatory processes [ 241 , 242 , 243 , 244 , 245 ]. AgNPs from the Piper nigrum extract were shown as selective cytokine inhibitory agents for IL-1β and IL-6 [ 246 ].…”

A protective effect of curcumin on cardiovascular oxidative stress indicators in systemic inflammation induced by lipopolysaccharide in rats

“…Although significant progress has been made in managing synthetic drugs for cardiovascular diseases, the search for indigenous cardioprotective agents is continuing. It has been shown that at least 30 food spices and herbs possess antioxidant properties such as Boswellia serrata , Nigella sativa, saffron and their major components such as thymoquinone, and safranol, [ [18] , [19] , [20] , [21] ]. Curcumin, the major yellow bioactive component of turmeric ( Curcuma longa L., Zingiberaceae), is a more recent discovery [ 22 , 23 ].…”