Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells

Anu, Kasi; Ganesan, Singaravelu; Murugan, Kadarkarai; Benelli, Giovanni

doi:10.1007/s10876-016-1123-7

Cited by 122 publications

(95 citation statements)

References 55 publications

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“…No appreciable decline in cell viability was observed at the tested concentrations (including MIC values), portraying their safety profile for antibacterial application [23,44].…”

Section: Biocompatibilitymentioning

confidence: 78%

“…The UV-vis spectra of the resulting nSe revealed a sharp characteristic peak at 272 nm, while no such peaks were observed in the absorption spectra of extract and Na 2 SeO 3 solution (figure 2). The appearance of colour and subsequent absorption peak of nSe was attributed to the surface plasmon resonance (SPR) phenomenon [30,44].…”

Section: Resultsmentioning

confidence: 99%

“…As compared to TEM, the larger size of nSe was observed in DLS studies because of the presence of extract coating as also evident by TEM analysis. Also, water molecules used as the solvent bound to the nSe surface may increase its size [44,49,54].…”

Section: Resultsmentioning

confidence: 99%

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Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μg ml −1 and 3.34 μg ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μg ml −1 ), Staphylococcus aureus (32 μg ml −1 ) and Pseudomonas aeruginosa (48 μg ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data obtained demonstrated the versatility of phytogreen nSe as a potent antioxidant and antibacterial agent to effectively prevent as well as treat multidrug-resistant bacterial infections.

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“…No appreciable decline in cell viability was observed at the tested concentrations (including MIC values), portraying their safety profile for antibacterial application [23,44].…”

Section: Biocompatibilitymentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

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Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

et al. 2020

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“…Reported literature studies revealed that, Na 2 SeO 3 solution (10 mM) was prepared by addition of 0.263 g of its salt into the 100 mL of DI water [2,4,17,18]. In typical synthesis, different amounts (1-5 mL) of ALE were mixed with various amounts (10-30 mL) of Na 2 SeO 3 solution.…”

Section: Fabrication Of the Se Nps Using Alementioning

confidence: 99%

“…Red colour of the reaction mixture solution indicates that the Se NPs by reduction of the Se ions and converting them to the elemental Se and formation of the Se NPs [21]. Several studies indicated that by synthesis of the Se NPs in the solution, its colour progressively varies from the dark yellow into the red [17,22]. The colour intensity of the colloidal solution containing synthesized Se NPs was measured using UV-Vis spectrophotometry adjusted at 420 nm (wavelength) and stated as absorbance unit (% a.u.…”

Section: Colour Intensity Of the Synthesized Se Npsmentioning

confidence: 99%

Aloe vera leaf extract mediated green synthesis of selenium nanoparticles and assessment of their In vitro antimicrobial activity against spoilage fungi and pathogenic bacteria strains

Fardsadegh

Jafarizadeh‐Malmiri

2019

Green Processing and Synthesis

161

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Selenium as an essential trace element for the health of the humans was used to hydrothermally synthesis of selenium nanoparticle (Se NPs) using Aloe vera leaf extract (ALE). Effects of synthesis parameters namely; amount of ALE (1–5 ml) and amount of Na2SeO3 solution (10–30 ml), on the particle size and colour intensity of the solution containing Se NPs were studied using response surface methodology. FT-IR spectroscopy, UV-Vis spectrophotometry, DLS analyzer and TEM were used to determine the specifications of the ALE and synthesized Se NPs. Obtained results indicated that the ALE contained several bioactive compounds, which they had hydroxyl and amide І groups in their structures and these two functional groups had key roles in the reduction of the selenium ions to form Se NPs and stabilizing of them. Furthermore, spherical fabricated Se NPs using obtained optimum synthesis parameters, 4.92 mL of ALE and 13.03 mL of Na2SeO3 solution, had colour intensity, mean particle size, zeta potential and polydispersity index values of 3.0% a.u., 50 nm, -18 mV and 0.344, respectively according to the DLS analysis. The synthesized Se NPs had also high antibacterial and antifungal activities against 4 selected pathogenic bacteria and spoilage fungi strains.

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Biogenic and facile synthesis of selenium nanoparticles using Vaccinium arctostaphylos L. fruit extract and anticancer activity against in vitro model of breast cancer

Gharbavi

Mousavi

Pour-Karim

et al. 2022

Cell Biology International

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Biogenic synthesis of selenium nanoparticles (SeNPs) using plant extracts has emerged as a promising alternative approach to traditional chemical synthesis. The current study aims to introduce a safe, low-cost, and green synthesis of SeNPs using fresh fruit extract of Vaccinium arctostaphylos L. The biogenic synthesis of SeNPs was confirmed by different analyses including ultraviolet-visible spectrophotometry, Fourier transform infrared, and energy-dispersive X-ray. Also, the crystalline nature, size, and morphology of the obtained SeNPs were characterized by X-ray diffraction, dynamic light scattering, field emission scanning electron microscopy, and transmission electron microscopy techniques. The SeNPs were successfully synthesized with fruit extract of V. arctostaphylos L. in a regular spherical form and narrow size distribution with suitable zeta-potential values and exhibited appropriate biocompatibility. It revealed that the synthesized SeNPs can significantly inhibit the growth of 4T1 breast cancer cells with an IC 50 of ∼84.19 ± 25.96 µg/ml after 72 h treatment. Overall, it can be concluded that the green synthesized SeNPs can be attractive, nontoxic, and eco-friendly candidates for drug delivery or medicinal applications.

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Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells

Cited by 122 publications

References 55 publications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Phytogreen synthesis of multifunctional nano selenium with antibacterial and antioxidant implications

Aloe vera leaf extract mediated green synthesis of selenium nanoparticles and assessment of their In vitro antimicrobial activity against spoilage fungi and pathogenic bacteria strains

Biogenic and facile synthesis of selenium nanoparticles using Vaccinium arctostaphylos L. fruit extract and anticancer activity against in vitro model of breast cancer

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