Green Formulation Strategy for Preparing Oil-in-Water Emulsions via Lipase-Catalyzed Transesterification

Abstract

Formulation of submicronic diglyceride-in-water emulsions was carried-out without addition of synthetic surfactant in case of commercial caprylic/capric diglyceride. Sugar surfactant was prepared by contacting the oil with a concentrated aqueous solution of sorbitol (70 wt %) containing lipase AY. Interfacial lipase-catalyzed transesterification took place and led to limited but sufficient amounts of sorbitol ester and monoglyceride, which accumulated in the oil. The enzyme-treated oil could be easily separated from the aqueous phase and used for preparing oil-in-water emulsion without adding any other surfactant. A stable emulsion was obtained for at least 7 days and exhibited better stability at 60 °C than Tween 20-stabilized emulsion.

Lipase‐catalyzed synthesis of sorbitol octanoate in aqueous biphasic medium and its use in a green formulation process of oil‐in‐water food nanoemulsions

“…This O/W food emulsion model had good stability after 7 days storage at 25°C or 60°C. Recent work examined the possible extension of this new strategy to triglyceride oils using lipase‐catalyzed transesterification . The possible application of the reported results to the manufacturing of food grade formulations is currently under investigation.…”

Reaction‐controlled microgel‐lipase co‐stabilized compartmentalized emulsion for recyclable biodiesel production

“…As an important enzyme, lipase plays a main role in multiple biological and chemical reactions, such as fat hydrolysis and biodiesel production. [29,30] Since lipase is soluble in water and the substances are dissolved in oil, the hydrolysis or esterification process mainly occurs at the oil-water interface. Therefore, a large interfacial area between the aqueous and organic phases must be ensured to enhance the efficiency of biphasic enzymatic reactions.…”