Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells

Cerisoli, Francesco; Cassinelli, Letizia; Lamorte, Giuseppe; Citterio, Stefania; Bertolotti, Francesca; Magli, Maria Cristina; Ottolenghi, Sergio

doi:10.3324/haematol.13689

Cited by 5 publications

(7 citation statements)

References 43 publications

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“…The Kit/GFP transgenic line expresses GFP from a construct driven by mouse Kit gene promoter and enhancer elements, linked to the GFP gene [25,26] The transgene is appropriately expressed in several Kit ϩ stem/ progenitor cell types, including primordial germ cells, early haematopoietic progenitors and stem cells (HSC) and CSCs [1,[25][26][27][28].…”

Section: Bm-derived Cells Are Able To Generate Cardiospheres In Vitromentioning

confidence: 99%

“…We addressed the question whether, following myocardial infarction (MI), BM cells contribute to myocardic tissue repair by the generation of a cell population with the functional features of CSCs. We combined two recent advancements made in our laboratories: (i ) first, the possibility to grow in vitro mouse CSCs, which extensively proliferate, transiently express Kit and generate beating CSs, capable of further cardiac differentiation [1,8]; (ii ) second, the generation of transgenic mouse line (Kit/GFP [green fluorescent protein]) that efficiently expresses GFP in a variety of stem and progenitor Kit ϩ cells, including BM and CSCs, under the control of mouse Kit regulatory DNA regions [25][26][27][28]. In the present work, we transplanted lethally irradiated mice with BM cells expressing the Kit/GFP transgene.…”

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confidence: 99%

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Bone marrow‐derived cells can acquire cardiac stem cells properties in damaged heart

Barile

Cerisoli

Frati

et al. 2011

J Cellular Molecular Medi

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Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit+ CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate ‘cardiospheres’, a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit+ CSCs.

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Section: Bm-derived Cells Are Able To Generate Cardiospheres In Vitromentioning

confidence: 99%

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confidence: 99%

Bone marrow‐derived cells can acquire cardiac stem cells properties in damaged heart

Barile

Cerisoli

Frati

et al. 2011

J Cellular Molecular Medi

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“…The c-KIT protein is primarily found on hematopoietic cells and is a marker of long-term reconstituting HSCs in humans [89] and mice [90][91][92]. c-KIT is expressed on pluripotent and multipotent HSCs and myeloerythroid precursors, but not on differentiating or mature cell types [90][91][92], with the exception of mast cells [93]. Approximately 2 x 10 4 c-KIT receptors are found on normal human HPCs [94], and can undergo proteolytic cleavage to release a soluble form of c-KIT [95][96][97].…”

Section: Mammalian Stem Cell Factor and Kit Receptormentioning

confidence: 99%

Regulation of Teleost Macrophage and Neutrophil Cell Development by Growth Factors and Transcription Factors

Katzenback¹,

Katakura²,

Belosevic³

2012

New Advances and Contributions to Fish Biology

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Additional information is available at the end of the chapter http://dx.doi.org/10.5772/53589 . IntroductionMacrophages and neutrophils are the sentinel cells of the innate immune response of vertebrates, such as bony fish teleosts . "s phagocytic myeloid cells, they are involved in homeostatic mechanisms, wound healing, and the detection, elimination and clearance of foreign entities including tumors, virus-infected cells and invading pathogens. Furthermore, macrophages and neutrophils are responsible for producing hundreds of bioactive molecules that are important in pathogen recognition and destruction, cellular communication and activation, initiation of an adaptive immune response and later, resolution of an inflammatory response and tissue repair. Neutrophils and macrophages, while essential to survival, have a finite lifespan. Therefore, a manufacturing centre, the hematopoietic niche, is needed for the production of myeloid cells. The hematopoietic niche must maintain basal myeloid cell production levels during homeostasis, yet retain the flexibility to ramp-up cell production in response to physiological demands, such as pathogenic insult. The development of macrophages monopoiesis and neutrophils granulopoiesis is collectively known as myelopoiesis, and is regulated by the complex interaction of colony-stimulating factors CSFs , their receptors, and intracellular transcription factor machinery that control lineage fate decisions and terminal differentiation events.Over the past years, research using the mouse model system has culminated in the identification of the site s of myelopoiesis, the progenitor cell types that give rise to mature myeloid cells, the extracellular and intracellular cues required, and a detailed understanding of the complex intracellular and extracellular milieu of factors that drive this tightly controlled © 2013 Katzenback et al.; licensee InTech. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.process. From these studies we understand hematopoiesis as an exquisitely fine-tuned, highly regulated, process whereby all blood cells develop from a small number of hematopoietic stem cells HSCs . HSCs are characterized as long-term repopulating, pluripotent, quiescent cells that undergo symmetrical self-renewal to sustain the population of HSCs within the hematopoietic niche, or asymmetrical division to give rise to hematopoietic progenitor cells HPCs [ ]. HPCs can develop along the lymphoid lineage, termed lymphopoiesis, to give rise to "-cells, T-cells, natural killer NK cells and dendritic cells DCs . "lternatively, HPCs can develop along an erythroid lineage, termed erythropoiesis, to give rise to erythrocytes and megakaryocytes, or develop along a myeloid lineage to give rise to granulocytes neutrophils, basophils, eosinophils, mast cells , mononuclear phagoc...

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“…After cell extraction, monolayer cell cultures were plated on cellular culture flasks of 75 cm 2 until total confluence. MSC were marked with green fluorescent protein (GFP), for easy fluorescence imaging detection as described by Cerisoli et al (2009) [39]. The experimental protocol was reviewed and approved by the Institution's Human Research Committee (protocol 2007.1.487.58.7, FFCLRP, São Paulo University-USP, Ribeirão Preto, São Paulo State, Brazil).…”

Section: Stem Dermal Equivalent (Sde)mentioning

confidence: 99%

“…Proteinase activity was evident as clear (unstained) zones. Finally the gels were incubated for one hour in 5% methanol, 7.5% acetic acid and kept under cellophane as previously described [39]. Fig.…”

Section: Mmp Quantification By Zymography Electrophoreticmentioning

confidence: 99%

Efeitos da Nanoemulsão de Ftalocianina Cloro-Alumínio na Regulação da Via do Fator de Crescimento Epidermal em Glioblastoma e Meduloblastoma

Paula¹

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Ao meu orientador, mestre e amigo Dr. Antonio Claudio Tedesco pela oportunidade de me agregar ao seu grupo e por compartilhar de seus conhecimentos que, sem dúvida, contribuíram para o meu crescimento seja ele pessoal e/ou profissional. Ter sido seu orientado me proporcionou experiências e aprendizados únicos que eu irei levar pelo resto de minha vida. O convívio no dia-adia proporcionou oportunidades de enriquecimento intelectual, pessoal e, principalmente, amadurecimento profissional. Sob sua orientação, a palavra profissionalismo sempre foi marcante em seus ensinamentos. Ser membro do Centro de Nanotecnologia e Engenharia Tecidual é um grande diferencial na minha vida profissional. Parabéns pelo empenho e competência frente à pesquisa, por todo apoio e suporte para o desenvolvimento desse trabalho e por sempre me incentivar a alcançar os meus objetivos. Não tenho dúvida de que você será o meu eterno mestre. Sempre terá o meu respeito e admiração. Ao Dr. Fernando Lucas Primo, pelo compartilhamento de sua experiência na vida acadêmica e pelos momentos de grandes ensinamentos para o meu crescimento profissional e pessoal. Aos amigos do Centro de Nanotecnologia e Engenharia Tecidual (CNET-USP). Muito obrigado!!

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Green fluorescent protein transgene driven by Kit regulatory sequences is expressed in hematopoietic stem cells

Cited by 5 publications

References 43 publications

Bone marrow‐derived cells can acquire cardiac stem cells properties in damaged heart

Bone marrow‐derived cells can acquire cardiac stem cells properties in damaged heart

Regulation of Teleost Macrophage and Neutrophil Cell Development by Growth Factors and Transcription Factors

Efeitos da Nanoemulsão de Ftalocianina Cloro-Alumínio na Regulação da Via do Fator de Crescimento Epidermal em Glioblastoma e Meduloblastoma

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