Graying the self-assembly of gold nanoparticles for improved enzyme activity assays

Kim, Gae Baik; Lee, Jin Oh; Kim, Young‐Pil

doi:10.1016/j.snb.2017.02.067

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…This development resulted in a threefold improvement in detection limit, but the additional steps are time consuming and add to costs. 119 Moreover, an approach by Valentini et al for the colorimetric detection of the cancer-related point mutation was demonstrated using AuNPs combined with signal enhancement on the surface of paramagnetic microparticles. This simple colorimetric assay is sensitive (with a limit of detection in the low picomolar range) and instrument-free.…”

Section: 116-118mentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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Gold nanoparticles (AuNPs) provide excellent platforms for the development of colorimetric biosensors as they can be easily functionalised, displaying different colours depending on their size, shape and state of aggregation. In the last decade, a variety of biosensors have been developed to exploit the extent of colour changes as nano-particles (NPs) either aggregate or disperse, in the presence of analytes. Of critical importance to the design of these methods is that the behaviour of the systems has to be reproducible and predictable. Much has been accomplished in understanding the interactions between a variety of substrates and AuNPs, and how these interactions can be harnessed as colorimetric reporters in biosensors. However, despite these developments, only a few biosensors have been used in practice for the detection of analytes in biological samples. The transition from proof of concept to market biosensors requires extensive long-term reliability and shelf life testing, and modification of protocols and design features to make them safe and easy to use by the population at large. Developments in the next decade will see the adoption of user friendly biosensors for point-of-care and medical diagnosis as innovations are brought to improve the analytical performances and usability of the current designs. This review discusses the mechanisms, strategies, recent advances and perspectives for the use of AuNPs as colorimetric biosensors.

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Section: 116-118mentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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“…[20][21][22][23][24] For protease detection, the peptide substrates are usually functionalized with one or more cysteine residues or positively charged amino acid residues including Lys and Arg. [25][26][27][28][29][30][31][32][33] Cleavage of a single peptide into two shorter fragments by the specific protease prevents the peptide substrate-induced aggregation or facilitates the fragment-triggered aggregation of AuNPs based on the Au-S and/or electrostatic interactions. Nonetheless, to trigger the AuNP aggregation, the peptide substrate must be well designed.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen

Xia¹,

Deng²,

Wang³

et al. 2018

IJN

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BackgroundProstate-specific antigen (PSA), a serine protease, is a biomarker for preoperative diagnosis and screening of prostate cancer and monitoring of its posttreatment.MethodsIn this work, we reported a colorimetric method for clinical detection of PSA using gold nanoparticles (AuNPs) as the reporters. The method is based on ascorbic acid (AA)-induced in situ formation of AuNPs and Cu2+-catalyzed oxidation of AA. Specifically, HAuCl4 can be reduced into AuNPs by AA; Cu2+ ion can catalyze the oxidation of AA by O2 to inhibit the formation of AuNPs. In the presence of the PSA-specific peptide (DAHSSKLQLAPP)-modified gold-coated magnetic microbeads (MMBs; denoted as DAHSSKLQLAPP-MMBs), complexation of Cu2+ by the MMBs through the DAH–Cu2+ interaction depressed the catalyzed oxidation of AA and thus allowed for the formation of red AuNPs. However, once the peptide immobilized on the MMB surface was cleaved by PSA, the DAHSSKLQ segment would be released. The resultant LAPP fragment remaining on the MMB surface could not sequestrate Cu2+ to depress its catalytic activity toward AA oxidation. Consequently, no or less AuNPs were generated.ResultsThe linear range for PSA detection was found to be 0~0.8 ng/mL with a detection limit of 0.02 ng/mL. Because of the separation of cleavage step and measurement step, the interference of matrix components in biological samples was avoided.ConclusionThe high extinction coefficient of AuNPs facilitates the colorimetric analysis of PSA in serum samples. This work is helpful for designing of other protease biosensors by matching specific peptide substrates.

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“…In this case, Au@Pt NPs showed a better catalytic activity and stability than HRP, and this sensing system exhibited a low LOD of 12.5 pg mL −1 . In other studies, metal ions were utilized as artificial co‐enzymes for enhancing the catalytic activity of Au NPs in colorimetric detection, and as a result, the sensitivity of the catalytic Au NP‐based sensing platform was improved in the presence of metal ions . In this case, metal ions and a mild reducing agent were added to the sensing solution after an antigen‐antibody/Au NP conjugation process, in which the metal ions were reduced and the resulting metal covered the surface of the Au NPs .…”

Section: Plasmonic and Catalytic Nanoparticle‐based Biosensing Systemsmentioning

confidence: 99%

High‐Performance Biosensing Systems Based on Various Nanomaterials as Signal Transducers

Lee

Adegoke

Park

2018

Biotechnology Journal

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Recently, highly sensitive and selective biosensors have become necessary for improving public health and well-being. To fulfill this need, high-performance biosensing systems based on various nanomaterials, such as nanoparticles, carbon nanomaterials, and hybrid nanomaterials, are developed. Numerous nanomaterials show excellent physical properties, including plasmonic, magnetic, catalytic, mechanical and fluorescence properties and high electrical conductivities, and these unique and beneficial properties have contributed to the fabrication of high-performance biosensors with various applications, including in optical, electrical, and electrochemical detection platforms. In addition, these properties can be transformed to signals for the detection of biomolecules. In this review, various types of nanomaterial-based biosensors are introduced, and they show high sensitivity and selectivity. In addition, the potential applications of these sensors on the biosensing of several types of biomolecules are also discussed. These nanomaterials-based biosensing systems provide a significant improvement on healthcare including rapid monitoring and early detection of infectious disease for public health.

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Graying the self-assembly of gold nanoparticles for improved enzyme activity assays

Cited by 9 publications

References 23 publications

Gold nanoparticle-based colorimetric biosensors

Gold nanoparticle-based colorimetric biosensors

Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen

High‐Performance Biosensing Systems Based on Various Nanomaterials as Signal Transducers

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