Graphite Nanoplatelets and Caenorhabditis elegans: Insights from an in Vivo Model

Zanni, Elena; Bellis, Giovanni De; Bracciale, Maria Paola; Broggi, Alessandra; Santarelli, María Laura; Sarto, Maria Sabrina; Palleschi, Claudio; Uccelletti, Daniela

doi:10.1021/nl204388p

Cited by 134 publications

(113 citation statements)

References 32 publications

(48 reference statements)

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“…[19][20][21] These NMs include metal nanoparticles (NPs; TiO 2 , Al 2 O 3 , and ZnO 2 ) [22][23][24] and carbon NPs (carbon NTs [CNTs], graphene oxide [GO], and graphite nanoplatelets). [25][26][27][28] Here, we analyze the two aforementioned representative forms of BN NMs in C. elegans: BNNSs and BN-800-2. In this study, we examined the multiple phenotypic toxicities of two types of BN NMs with different morphology, size, and solubility using C. elegans in aqueous media.…”

Section: Wang Et Almentioning

confidence: 99%

Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans

Wang¹,

Wang

Tang

et al. 2017

IJN

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Boron nitride (BN) nanomaterials have been increasingly explored for potential biological applications. However, their toxicity remains poorly understood. Using Caenorhabditis elegans as a whole-animal model for toxicity analysis of two representative types of BN nanomaterials – BN nanospheres (BNNSs) and highly water-soluble BN nanomaterial (named BN-800-2) – we found that BNNSs overall toxicity was less than soluble BN-800-2 with irregular shapes. The concentration thresholds for BNNSs and BN-800-2 were 100 µg·mL −1 and 10 µg·mL −1 , respectively. Above this concentration, both delayed growth, decreased life span, reduced progeny, retarded locomotion behavior, and changed the expression of phenotype-related genes to various extents. BNNSs and BN-800-2 increased oxidative stress levels in C. elegans by promoting reactive oxygen species production. Our results further showed that oxidative stress response and MAPK signaling-related genes, such as GAS1 , SOD2 , SOD3 , MEK1 , and PMK1 , might be key factors for reactive oxygen species production and toxic responses to BNNSs and BN-800-2 exposure. Together, our results suggest that when concentrations are lower than 10 µg·mL −1 , BNNSs are more biocompatible than BN-800-2 and are potentially biocompatible material.

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Section: Wang Et Almentioning

confidence: 99%

Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans

Wang¹,

Wang

Tang

et al. 2017

IJN

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“…Graphite has also been widely considered as benign for various biomedical use. [ 16 ] Initially, bare carbon ink was prepared by thoroughly dissolving 30 wt% graphite in PEG solution (60 wt% in water). It was observed that higher graphite or PEG content led to clogging of the pen.…”

Section: Rationale For Enzymatic-ink Compositionmentioning

confidence: 99%

Biocompatible Enzymatic Roller Pens for Direct Writing of Biocatalytic Materials: “Do‐it‐Yourself” Electrochemical Biosensors

Bandodkar

Jia

Ramírez

et al. 2015

Adv Healthcare Materials

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The development of enzymatic-ink-based roller pens for direct drawing of biocatalytic sensors, in general, and for realizing renewable glucose sensor strips, in particular, is described. The resulting enzymatic-ink pen allows facile fabrication of high-quality inexpensive electrochemical biosensors of any design by the user on a wide variety of surfaces having complex textures with minimal user training. Unlike prefabricated sensors, this approach empowers the end user with the ability of "on-demand" and "on-site" designing and fabricating of biocatalytic sensors to suit their specific requirement. The resulting devices are thus referred to as "do-it-yourself" sensors. The bio-active pens produce highly reproducible biocatalytic traces with minimal edge roughness. The composition of the new enzymatic inks has been optimized for ensuring good biocatalytic activity, electrical conductivity, biocompati-bility, reproducible writing, and surface adherence. The resulting inks are characterized using spectroscopic, viscometric, electrochemical, thermal and microscopic techniques. Applicability to renewable blood glucose testing, epidermal glucose monitoring, and on-leaf phenol detection are demonstrated in connection to glucose oxidase and tyrosinase-based carbon inks. The "do-it-yourself" renewable glucose sensor strips offer a "fresh," reproducible, low-cost biocatalytic sensor surface for each blood test. The ability to directly draw biocatalytic conducting traces even on unconventional surfaces opens up new avenues in various sensing applications in low-resource settings and holds great promise for diverse healthcare, environmental, and defense domains.

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“…Here a primary imaging method based on Fourier transform infrared spectroscopy (FTIR) mapping has already been preferentially exploited in structural elucidation to investigate the probable interplays and complicated interactions between ALA and lipids during the course of preparation of ALA-NLC aqueous dispersion (Zanni et al, 2012). The method has obtained significant importance in the near future since it allows the occasion to completely comprehend the configuration of biological molecules (Palaniappan et al, 2011).…”

Section: Qualitative Features Of the Ftir Spectra Of The Nlc Systemmentioning

confidence: 99%

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

Wang

Xia²

2013

Drug Delivery

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ALA-loaded nanostructured lipid carrier (ALA-NLC) was designed to improve physicochemical stability and water solubility, and promote sustained release of ALA as well as determine the biocompatibility of ALA-NLC. The ALA-NLC manufactured using hot high-pressure homogenization technique was investigated in terms of size, zeta potential, FTIR analysis and release behavior. In vitro cytotoxicity and biocompatibility were determined by incubating with HaCaT cells using the MTT assay, HE staining and Hoechst 33342 staining. Cell behavior and cellular division of HaCaT cells untreated and treated by ALA-NLC were investigated in real-time images gathered using time-lapse imaging system. The release investigation illustrated that only 6.9% of ALA released in 30 min from ALA-NLC formation, whereas it was 30.3% in free ALA system. ALA-NLC possessed a satisfactory release behavior of sustained release up to 72 h. It showed that ALA-NLC did not exert hazardous effect on HaCaT cells up to 81.9 mg/L without morphological alterations, revealing a satisfactory biocompatibility. Evidence was provided from time-lapse imaging system that cell behavior and cellular division of ALA-NLC treated HaCaT cells were in accordance with the control. These results of this investigation demonstrated that NLC encapsulated ALA formation (ALA-NLC) can improve stability, solubility and release of ALA; ALA-NLC was biocompatible to HaCaT cells.

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Graphite Nanoplatelets and Caenorhabditis elegans: Insights from an in Vivo Model

Cited by 134 publications

References 32 publications

Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in <em>Caenorhabditis elegans</em>

Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in <em>Caenorhabditis elegans</em>

Biocompatible Enzymatic Roller Pens for Direct Writing of Biocatalytic Materials: “Do‐it‐Yourself” Electrochemical Biosensors

Alpha-lipoic acid-loaded nanostructured lipid carrier: sustained release and biocompatibility to HaCaT cellsin vitro

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