Graphene oxide nanoparticle attachment and its toxicity on living lung epithelial cells

Ahlinder, Linnéa; Henych, Jiří; Lindström, Susanne Wiklund; Ekstrand‐Hammarström, Barbro; Štengl, Václav; Österlund, Lars

doi:10.1039/c5ra09351a

Cited by 11 publications

(13 citation statements)

References 53 publications

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“…Furthermore, it was found that the hydrophilic form of GF like GO can form a stable colloid dispersion and thus avoids aggregation, which is the key to easy removal from the application site. However, even though GO are more hydrophilic, some early studies showed that these nanosheets exhibited time and dose-dependent cytotoxicity, and managed to enter into the cytoplasm and nucleus, decreasing cell adhesion, and inducing cell floating and apoptosis [ 54 , 55 , 56 , 57 , 58 ]. Thus, surface functionalization can play a major role in providing GO appropriate vehicles for several therapeutics like drug molecules, and especially anticancer drugs, DNA, genes, antibodies, antibacterial, proteins, etc.…”

Section: Types Of Nanocarriersmentioning

confidence: 99%

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Sıafaka

Okur

Karavas

et al. 2016

IJMS

156

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Nanocarriers, due to their unique features, are of increased interest among researchers working with pharmaceutical formulations. Polymeric nanoparticles and nanocapsules, involving non-toxic biodegradable polymers, liposomes, solid lipid nanoparticles, and inorganic–organic nanomaterials, are among the most used carriers for drugs for a broad spectrum of targeted diseases. In fact, oral, injectable, transdermal-dermal and ocular formulations mainly consist of the aforementioned nanomaterials demonstrating promising characteristics such as long circulation, specific targeting, high drug loading capacity, enhanced intracellular penetration, and so on. Over the last decade, huge advances in the development of novel, safer and less toxic nanocarriers with amended properties have been made. In addition, multifunctional nanocarriers combining chemical substances, vitamins and peptides via coupling chemistry, inorganic particles coated by biocompatible materials seem to play a key role considering that functionalization can enhance characteristics such as biocompatibility, targetability, environmental friendliness, and intracellular penetration while also have limited side effects. This review aims to summarize the “state of the art” of drug delivery carriers in nanosize, paying attention to their surface functionalization with ligands and other small or polymeric compounds so as to upgrade active and passive targeting, different release patterns as well as cell targeting and stimuli responsibility. Lastly, future aspects and potential uses of nanoparticulated drug systems are outlined.

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Section: Types Of Nanocarriersmentioning

confidence: 99%

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Sıafaka

Okur

Karavas

et al. 2016

IJMS

156

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“…Confocal Raman spectroscopy is an analytical method which has demonstrated great utility in a wide range of applications in life science applications, such as cancer diagnostics [ 1 ] and—as exemplified in this study—detection of particles inside cells [ 2 , 3 , 4 , 5 , 6 , 7 ]. Some advantages are that the method requires minimal sample preparation, is label free and non-destructive [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Furthermore, it is possible to collect hyperspectral images of the sample by step-wise measurements of small sample volumes [ 4 , 5 , 6 , 7 , 8 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, it is possible to collect hyperspectral images of the sample by step-wise measurements of small sample volumes [ 4 , 5 , 6 , 7 , 8 , 10 , 11 ]. Each pixel in such a (hyperspectral) Raman image contains a Raman spectrum with chemical information from molecules and crystals with Raman active vibrational modes [ 2 , 3 , 4 , 5 , 6 , 7 , 9 ]. It is possible to simultaneously detect particles inside cells [ 2 , 3 , 4 , 5 , 6 , 7 ] and to use the spectral information to discern organelles without using dyes, fluorescent labels, or microtome sectioning and associated embedding methods [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Each pixel in such a (hyperspectral) Raman image contains a Raman spectrum with chemical information from molecules and crystals with Raman active vibrational modes [ 2 , 3 , 4 , 5 , 6 , 7 , 9 ]. It is possible to simultaneously detect particles inside cells [ 2 , 3 , 4 , 5 , 6 , 7 ] and to use the spectral information to discern organelles without using dyes, fluorescent labels, or microtome sectioning and associated embedding methods [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Besides providing a better understanding of the particle toxicity [ 2 , 3 , 4 , 5 ], Raman images of cells exposed to nanoparticles can be useful in the development of nanomaterials for drug delivery applications and diagnostics [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Noise Removal with Maintained Spatial Resolution in Raman Images of Cells Exposed to Submicron Polystyrene Particles

et al. 2016

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The biodistribution of 300 nm polystyrene particles in A549 lung epithelial cells has been studied with confocal Raman spectroscopy. This is a label-free method in which particles and cells can be imaged without using dyes or fluorescent labels. The main drawback with Raman imaging is the comparatively low spatial resolution, which is aggravated in heterogeneous systems such as biological samples, which in addition often require long measurement times because of their weak Raman signal. Long measurement times may however induce laser-induced damage. In this study we use a super-resolution algorithm with Tikhonov regularization, intended to improve the image quality without demanding an increased number of collected pixels. Images of cells exposed to polystyrene particles have been acquired with two different step lengths, i.e., the distance between pixels, and compared to each other and to corresponding images treated with the super-resolution algorithm. It is shown that the resolution after application of super-resolution algorithms is not significantly improved compared to the theoretical limit for optical microscopy. However, to reduce noise and artefacts in the hyperspectral Raman images while maintaining the spatial resolution, we show that it is advantageous to use short mapping step lengths and super-resolution algorithms with appropriate regularization. The proposed methodology should be generally applicable for Raman imaging of biological samples and other photo-sensitive samples.

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“…Commercially prepared quantum dots based on Cd sulfides or selenides without surface treatment for their toxicity are not suitable for biological applications. The toxicity and biosafety materials of carbon nanostructures such as carbon nanotubes, graphene, graphene oxide [1,2], carbon, graphene quantum dots [3], iron oxide, and titanium oxide [4] are intensively under study.…”

Section: Introductionmentioning

confidence: 99%

Fast and Straightforward Synthesis of Luminescent Titanium(IV) Dioxide Quantum Dots

Štengl

Henych

Šťastný

et al. 2017

Journal of Nanomaterials

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The nucleus of titania was prepared by reaction of solution titanium oxosulphate with hydrazine hydrate. These titania nuclei were used for titania quantum dots synthesis by a simple and fast method. The prepared titanium(IV) dioxide quantum dots were characterized by measurement of X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), high-resolution electron microscopy (HRTEM), and selected area electron diffraction (SAED). The optical properties were determined by photoluminescence (PL) spectra. The prepared titanium(IV) dioxide quantum dots have the narrow range of UV excitation (365-400 nm) and also a close range of emission maxima (450-500 nm).

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Graphene oxide nanoparticle attachment and its toxicity on living lung epithelial cells

Abstract: Since its discovery graphene and its oxidized form graphene oxide have attracted interest in a wide range of applications, which calls for scrutinized studies about their possible toxicity.

Cited by 11 publications

References 53 publications

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Surface Modified Multifunctional and Stimuli Responsive Nanoparticles for Drug Targeting: Current Status and Uses

Noise Removal with Maintained Spatial Resolution in Raman Images of Cells Exposed to Submicron Polystyrene Particles

Fast and Straightforward Synthesis of Luminescent Titanium(IV) Dioxide Quantum Dots

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