Grapevine tissues and phenology differentially affect soluble carbohydrates determination by capillary electrophoresis

Moreno, Daniela; Píccoli, Patricia; Silva, María Fernanda

doi:10.1016/j.plaphy.2017.07.010

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…CE-MS has been applied to the study of changes in the primary metabolism of tobacco leaves according to their geographical location [84] and to quantifying organic acids in coffee seeds [85]. Similarly, the most abundant sugars in mature and young leaves, fruits and roots of grapevine were assessed using CE [87]. However, the low sensitivity [88] and poor reproducibility [89] of CE has minimized the use of this platform in plant research.…”

Section: Separation and Detectionmentioning

confidence: 99%

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

et al. 2021

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Metabolomics is a technology that generates large amounts of data and contributes to obtaining wide and integral explanations of the biochemical state of a living organism. Plants are continuously affected by abiotic stresses such as water scarcity, high temperatures and high salinity, and metabolomics has the potential for elucidating the response-to-stress mechanisms and develop resistance strategies in affected cultivars. This review describes the characteristics of each of the stages of metabolomic studies in plants and the role of metabolomics in the characterization of the response of various plant species to abiotic stresses.

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Section: Separation and Detectionmentioning

confidence: 99%

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

et al. 2021

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“…Using these extremely alkaline buffers causes deprotonation of the carbohydrates and provides a negative charge. The technique has been applied for the charge-to-size ratio separation of mono- and disaccharides in a wide variety of food and plants, such as honey, breakfast cereals, juice and nectar from fruits, and others. − While most of these analyses rely on direct ,,, or indirect ,,, detection by UV absorbance, one study demonstrated the use of capacitively coupled contactless conductivity detection coupled with electrospray ionization MS, which the authors suggest can be used for the detection of neutral species that may be overlooked in electrospray ionization MS due to poor ionization efficiency.…”

Section: Applicationsmentioning

confidence: 99%

Capillary Electrophoresis Separations of Glycans

et al. 2018

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Capillary electrophoresis has emerged as a powerful approach for carbohydrate analyses since 2014. The method provides high resolution capable of separating carbohydrates by charge-to-size ratio. Principle applications are heavily focused on N-glycans, which are highly relevant to biological therapeutics and biomarker research. Advances in techniques used for N-glycan structural identification include migration time indexing and exoglycosidase and lectin profiling, as well as mass spectrometry. Capillary electrophoresis methods have been developed that are capable of separating glycans with the same monosaccharide sequence but different positional isomers, as well as determining whether monosaccharides composing a glycan are alpha or beta linked. Significant applications of capillary electrophoresis to the analyses of N-glycans in biomarker discovery and biological therapeutics are emphasized with a brief discussion included on carbohydrate analyses of glycosaminoglycans and mono-, di-, and oligosaccharides relevant to food and plant products. Innovative, emerging techniques in the field are highlighted and the future direction of the technology is projected based on the significant contributions of capillary electrophoresis to glycoscience from 2014 to the present as discussed in this review.

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“…To date, a series of CE‐AD systems have been developed for biogenic amines , carbohydrates and phenol compounds , etc. which possessed the certain functional groups and electro‐active nature to a certain extent.…”

Section: Introductionmentioning

confidence: 99%

Sensitive amperometric detection for capillary electrophoresis of phenol carbamates with in‐line thermal hydrolysis strategy

et al. 2019

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A strategy on amperometric detection for CZE of phenol carbamates as model analytes with a facile in‐line thermal hydrolysis was presented, in which a thermal hydrolysis, subsequent CZE separation and final column‐end amperometric detection were accomplished in an intact capillary. Key parameters of hydrolysis dynamics of carbamates and electrochemical detection of the hydrolysates were studied, as well as electrophoretic conditions. Under the optimal conditions, the capillary was utilized as chambers for in situ hydrolysis, CZE separation, and electrochemical detection. The successive separation of hydrolysates of five carbamates (propoxur, carbofuran, 3‐OH‐carbofuran, carbaryl and bendiocarb) were achieved within 17 min. Applied to vegetable samples, the recoveries of carbamates fortified at 0.02 and 0.05 mg/kg were ranging in 88–107.2 and 86.3–107.3%, respectively. The success in the implementation of such a scheme resulted in a simple instrument as compared with those current analytical methods with post‐column derivization or pre‐column hydrolysis, or online enrichment in chip, respectively. This protocol might possess a potential utility for the sensitive amperometric detection of phenol carbamates.

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Grapevine tissues and phenology differentially affect soluble carbohydrates determination by capillary electrophoresis

Cited by 6 publications

References 45 publications

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

Capillary Electrophoresis Separations of Glycans

Sensitive amperometric detection for capillary electrophoresis of phenol carbamates with in‐line thermal hydrolysis strategy

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