Granulocyte—Macrophage Colony-Stimulating Factor Enhances the Humoral Immune Responses of Mouse Zona Pellucida 3 Vaccine Strategy Based on DNA and Protein Coadministration in BALB/c Mice

Kadir, Zibirnisa; Ma, Xiaolin; Li, Jinyao; Zhang, Fuchun

doi:10.1177/1933719112459236

Cited by 6 publications

(7 citation statements)

References 31 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modified FCA has also been used as a safe, effective substitute for FCA (Lyda et al 2005). Recent studies have also shown that intra-nasal delivery of four doses of mouse ZP3 result in a significant reduction of reproductive output in mice (Ma et al 2012;Kadir et al 2013). In parallel, several newer alternative reagents, such as purified and/or receptor-specific adjuvants (e.g.…”

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

View full text Add to dashboard Cite

Abstract. As human populations grow, conflicts with wildlife increase. Concurrently, concerns about the welfare, safety and environmental impacts of conventional lethal methods of wildlife management restrict the options available for conflict mitigation. In parallel, there is increasing interest in using fertility control to manage wildlife. The present review aimed at analysing trends in research on fertility control for wildlife, illustrating developments in fertility-control technologies and delivery methods of fertility-control agents, summarising the conclusions of empirical and theoretical studies of fertility control applied at the population level and offering criteria to guide decisions regarding the suitability of fertility control to mitigate human-wildlife conflicts. The review highlighted a growing interest in fertility control for wildlife, underpinned by increasing numbers of scientific studies. Most current practical applications of fertility control for wild mammals use injectable single-dose immunocontraceptive vaccines mainly aimed at sterilising females, although many of these vaccines are not yet commercially available. One oral avian contraceptive, nicarbazin, is commercially available in some countries. Potential new methods of remote contraceptive delivery include bacterial ghosts, virus-like particles and genetically modified transmissible and non-transmissible organisms, although none of these have yet progressed to field testing. In parallel, new species-specific delivery systems have been developed. The results of population-level studies of fertility control indicated that this approach may increase survival and affect social and spatial behaviour of treated animals, although the effects are species-and context-specific. The present studies suggested that a substantial initial effort is generally required to reduce population growth if fertility control is the sole wildlife management method. However, several empirical and field studies have demonstrated that fertility control, particularly of isolated populations, can be successfully used to limit population growth and reduce human-wildlife conflicts. In parallel, there is growing recognition of the possible synergy between fertility control and disease vaccination to optimise the maintenance of herd immunity in the management of wildlife diseases. The review provides a decision tree that can be used to determine whether fertility control should be employed to resolve specific human-wildlife conflicts. These criteria encompass public consultation, considerations about animal welfare and feasibility, evaluation of population responses, costs and sustainability.

show abstract

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

Fertility control to mitigate human–wildlife conflicts: a review

2014

View full text Add to dashboard Cite

show abstract

“…One hundred microliters of DMSO was added to each well, and the absorbance was measured at 570 nm using a microplate spectrophotometer (Benchmark Plus™, Bio-Rad). Data are reported as stimulation index, calculated as the mean absorbance of triplicate wells stimulated with an antigen, and divided by the mean absorbance of triplicate wells stimulated with the medium (A antigen /A control , used as control) ( 27 , 28 ).…”

Section: Methodsmentioning

confidence: 99%

“…Mucosal vaccination has the advantage of needle-free administration, induces both systemic and mucosal immune responses, and can be used for mass vaccination ( 25 ). Our previous studies showed that nasal immunization induced both IgA and IgG Ab responses to mZP3 in mouse models ( 27 , 28 ). In order to protect DNA from degradation, chitosan, a biodegradable cationic polysaccharide, was used to encapsulate the plasmid DNA as in our previous studies ( 14 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

Kadir

Zhang

2013

Braz J Med Biol Res

View full text Add to dashboard Cite

It has been shown that cytokines can act as molecular adjuvant to enhance the immune response induced by DNA vaccines, but it is unknown whether interleukin 33 (IL-33) can enhance the immunocontraceptive effect induced by DNA vaccines. In the present study, we explored the effects of murine IL-33 on infertility induced by Lagurus lagurus zona pellucida 3 (Lzp3) contraceptive DNA vaccine administered by the mucosal route. Plasmid pcD-Lzp3 and plasmid pcD-mIL-33 were encapsulated with chitosan to generate the nanoparticle chi-(pcD-Lzp3+pcD-mIL-33) as the DNA vaccine. Sixty female ICR mice, divided into 5 groups (n=12/group), were intranasally immunized on days 0, 14, 28, and 42. After intranasal immunization, the anti-LZP3-specific IgG in serum and IgA in vaginal secretions and feces were determined by ELISA. The results showed that chi-(pcD-Lzp3+pcD-mIL-33) co-immunization induced the highest levels of serum IgG, secreted mucosal IgA, and T cell proliferation. Importantly, mice co-immunized with chi-(pcD-Lzp3+pcD-mIL-33) had the lowest birth rate and mean litter size, which correlated with high levels of antibodies. Ovaries from infertile female mice co-immunized with chi-(pcD-Lzp3+pcD-mIL-33) showed abnormal development of ovarian follicles, indicated by atretic follicles and loss of oocytes. Our results demonstrated that intranasal delivery of the molecular adjuvant mIL-33 with chi-pcD-Lzp3 significantly increased infertility by enhancing both systemic and mucosal immune responses. Therefore, chi-(pcD-Lzp3+pcD-mIL-33) co-immunization could be a strategy for controlling the population of wild animal pests.

show abstract

“…Therefore, administration of ZP3 antibody can inhibit the interac-tion between sperm and eggs [3]. Aulanni'am and Sumitro found that ZP3 antibody was an effective inhibiting interaction between sperm and eggs in vivo fertilization by disrupting ZP3 binding to its ligand [4]. ZP3antibody based-contraception is also permanent and reversible [5].…”

Section: Introductionmentioning

confidence: 99%

“…Development of immunocontraception of pellucid zone among mammals, both native and recombinant, is widely developed from cows, goats, or rabbits. It shows the interspecific reaction of antibody on ZP3 [4]. Mubarakati et al produced ZP3 antibody from human blood (mab-hZP3) as a potential candidate for immunocontraception, but its side effect on folliculogenesis still need to be further studied [12].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary

Natalina

Nurseta

Winarsih

2018

J.Trop.Life.Science

View full text Add to dashboard Cite

Pellucid zone 3 (ZP3) involves in fertilization mechanism. Moreover, an antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female BALB/c mice were divided into 12 groups which consisted of a control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each group was treated differently: 50 µL adjuvant Al(OH) 3 in 50 µL Tris HCl, 20 µg Mab-hZP3, 40 µg Mab-hZP3, and 60 µg Mab-hZP3. Each group was then dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data were then analyzed by Two-Way ANOVA. The result showed that there was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p = 0.0825), and Bax (p = 0.836). There was no significant effect of administration of Mab-hZP3 in time (p = 0.807) on Bcl-2 expression (p = 0.088) but the significance difference was found in Bax level (p = 0.031). The lowest Bcl-2 level was found in a dose of 60 µg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p = 0.691), neither to Bcl-2 and Bax. The results obtained due to the specificity of a monoclonal antibody that recognizes a specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.

show abstract

Granulocyte—Macrophage Colony-Stimulating Factor Enhances the Humoral Immune Responses of Mouse Zona Pellucida 3 Vaccine Strategy Based on DNA and Protein Coadministration in BALB/c Mice

Cited by 6 publications

References 31 publications

Fertility control to mitigate human–wildlife conflicts: a review

Fertility control to mitigate human–wildlife conflicts: a review

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary

Contact Info

Product

Resources

About