Granulocytapheresis with modified fluid gelatin versus high‐molecular‐weight hydroxyethyl starch: a matched‐pair analysis

Dullinger, Katharina; Pamler, Irene; Brosig, Andreas; Mohrez, Morad; Hähnel, Viola; Offner, Robert; Dormann, Frauke; Becke, Christine; Holler, Ernst; Ahrens, Norbert

doi:10.1111/trf.13898

Cited by 14 publications

(18 citation statements)

References 31 publications

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Section: Discussionmentioning

confidence: 99%

“…Studies indicate that MFG produces lower granulocyte yields and results in granulocyte products with comparable RBC contents but higher platelet contamination levels than HES because MFG achieves less distinct cell separation . The lower harvest efficiency of MFG leads to granulocyte concentrates of inferior quality due to granulocyte aging if more than one donation day was scheduled . All in all, the use of MFG as a red blood cell sedimentation agent can be considered a safe and practicable method for the purification of neutrophil collections for granulocyte transfusion …”

Section: Discussionmentioning

confidence: 99%

“…The lower harvest efficiency of MFG leads to granulocyte concentrates of inferior quality due to granulocyte aging if more than one donation day was scheduled . All in all, the use of MFG as a red blood cell sedimentation agent can be considered a safe and practicable method for the purification of neutrophil collections for granulocyte transfusion …”

Section: Discussionmentioning

confidence: 99%

“…Modified fluid gelatin (MFG), a high‐molecular‐weight protein produced by hydrolysis of collagen, is already used as an alternative. Although MFG results in lower granulocyte yields and separates granulocytes from RBCs less distinctly than HES, it has been proven safe …”

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confidence: 99%

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Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function

et al. 2019

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BACKGROUND: Patients with neutropenia or granulocyte dysfunction may require granulocyte transfusions for adequate immune restoration. High-molecular-weight hydroxyethyl starch (HES) is the most commonly used sedimentation agent to enhance granulocyte collection efficiency. However, authorities recently restricted the use of HES due to its unfavorable risk-benefit profile. As modified fluid gelatin (MFG) is already used as an alternative sedimentation agent, we tested the hypothesis that MFG is not inferior to HES in terms of the functionality and viability of granulocytes. STUDY DESIGN AND METHODS:Granulocytes from ten healthy donors were isolated, aliquoted and incubated in parallel for 2 hours with either 0% (control), 7.5%, 15%, or 30% MFG (Gelafundin) or HES (Hespan), respectively, and granulocyte migration, chemotaxis, reactive oxygen species (ROS) production, neutrophil extracellular trap formation (NETosis), antigen expression, and viability were subsequently investigated in vitro. RESULTS:Relative to the controls, all three concentrations of HES compared to only 15% and 30% MFG lowered migration distances, and the 15% and 30% concentrations of both sedimentation agents reduced track straightness. HES resulted in lower CD11b expression and higher CD62L expression compared to MFG and the controls, whereas the differences for CD66b did not reach statistical significance. No significant differences in the timing of ROS production or NETosis, or in neutrophil viability or respiratory burst were observed. CONCLUSION:These results indicate that MFG is not inferior to HES in terms of granulocyte function in vitro when used at equal concentrations, and that potential impairment of granulocyte function can occur with HES.From the

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function

et al. 2019

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“…The assembled dataset was used to calculate matched molecular pairs (MMPs). The matched molecular pair technique has been used in the analysis of many properties . In the case of this study, the transformed part of the molecule has no more than 10 atoms, and fewer atoms than the main scaffold of the molecule .…”

Section: Methodsmentioning

confidence: 99%

Matched Molecular Pair Analysis on Large Melting Point Datasets: A Big Data Perspective

2017

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A matched molecular pair (MMP) analysis was used to examine the change in melting point (MP) between pairs of similar molecules in a set of ∼275k compounds. We found many cases in which the change in MP (ΔMP) of compounds correlates with changes in functional groups. In line with the results of a previous study, correlations between ΔMP and simple molecular descriptors, such as the number of hydrogen bond donors, were identified. In using a larger dataset, covering a wider chemical space and range of melting points, we observed that this method remains stable and scales well with larger datasets. This MMP‐based method could find use as a simple privacy‐preserving technique to analyze large proprietary databases and share findings between participating research groups.

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Einsatz von Hydroxyethylstärke (HES) als Sedimentationsbeschleuniger bei der Gewinnung von Granulozytenkonzentraten

2020

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Granulocytapheresis with modified fluid gelatin versus high‐molecular‐weight hydroxyethyl starch: a matched‐pair analysis

Cited by 14 publications

References 31 publications

Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function

Impact of hydroxyethyl starch and modified fluid gelatin on granulocyte phenotype and function

Matched Molecular Pair Analysis on Large Melting Point Datasets: A Big Data Perspective

Einsatz von Hydroxyethylstärke (HES) als Sedimentationsbeschleuniger bei der Gewinnung von Granulozytenkonzentraten

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